확장용매화이론을 사용한 마그네슘 및 코발트이온과 인간성장호르몬과의 상호작용에 대한 열역학적 연구
Using the Extended Solvation theory for Thermodynamic Study on the interaction of Magnesium and Cobalt ions with human growth hormone
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A thermodynamic study on the interaction between magnesium and cobalt ions (M2+), and human growth hormone, hGH, was studied at 27 °C in NaCl solution (50 mM) using the isothermal titration calorimetry. Isothermal titration calorimetry was applied to obtain the binding isotherm for hGH+M2+. The results obtained indicate that there is a set of three identical and noninteracting binding sites for Cobalt ions and a set of two for magnesium. The extended solvation model was used to reproduce the enthalpies of M2++hGH interactions over the whole metal ions concentrations. The solvation parameters recovered from the solvation model were attributed to the structural change of hGH due to the metal ion interaction. The extended solvation model was applied to elucidate the effect of Mg2+ and Co2+ binding on the protein stability.
Abstract
A thermodynamic study on the interaction between magnesium and cobalt ions (M2+), and human growth hormone, hGH, was studied at 27 °C in NaCl solution (50 mM) using the isothermal titration calorimetry. Isothermal titration calorimetry was applied to obtain the binding isotherm for hGH+M2+. The results obtained indicate that there is a set of three identical and noninteracting binding sites for Cobalt ions and a set of two for magnesium. The extended solvation model was used to reproduce the enthalpies of M2++hGH interactions over the whole metal ions concentrations. The solvation parameters recovered from the solvation model were attributed to the structural change of hGH due to the metal ion interaction. The extended solvation model was applied to elucidate the effect of Mg2+ and Co2+ binding on the protein stability.
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