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기계적 자극이 치주인대 세포의 osteoprotegerin과 receptor activator of nuclear factor κB ligand mRNA 발현에 미치는 영향

The effect of progressive tensional force on mRNA expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in the human periodontal ligament cell

황충주|이승일|옥승호|이기주|전옥순
대한치과교정학회 (The Korean Association Of Orthodontists)|2005.08|대한치과교정학회지 (The Korean Journal of Orthodontics)|제35권 제4호|pp. 262-274

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키워드

치주인대세포인장력OsteoprotegerinPeriodontal ligament cellMechanical strainReceptor activator of nuclear factor B ligand
주제분류치의학

초록

본 연구는 치주인대 세포에 지속적이고 점진적 인장력을 가하여 치아 이동 시 형성되는 인장부위의 기계적 자극에 대한 생화학적 전달과 치조골 흡수와 생성 조절 기전을 이해하고자 하였다. 치주인대 세포가 배양된 유연한 성장표면을 가진 배지에 지속적이고 점진적인 인장력을 가하고 골흡수 인자인 PGE2와 골형성 인자인 ALP의 생성량을 1, 3, 6, 12시간 후에 측정하여 정량비교하였고 파골세포 분화기전을 조절하는 OPG, RANKL의 인자들과 matrix metalloproteinase (MMP)-1, -8, -9, -13, tissue inhibitor of matrix metalloproteinase (TIMP)-1의 인자들을 역전사 중합효소 연쇄반응 검사하여 m-RNA 발현을 비교한 결과, 치주인대 세포에 인장력을 가한 경우 대조군보다 PGE2의 농도가 적었고 (p < 0.05) ALP의 농도 변화는 없었으며 OPG의 mRNA 발현이 증가하였으나, RANKL의 mRNA 발현은 감소하였다. 그리고 TIMP-1과 MMP-1, -8, -9, -13의 mRNA 발현이 대조군과 차이가 없었다. 이상의 연구에서 사람의 치주인대 세포는 점진적이고 지속적인 인장력에 대한 반응으로 PGE2의 생성과 RANKL의 mRNA 발현은 감소하고 OPG의 mRNA 발현은 증가하여 골흡수를 억제하는 효과를 보이는 것으로 나타났다.

Abstract

Tooth movement is a result of mutual physiologic responses between the periodontal ligament and alveolar bone stimulated by mechanical strain. The PDL cell and osteoblast are known to have an influence on bone formation by controlling collagen synthesis and alkaline phosphatase activation. Moreover, recent studies have shown that the PDL cell and osteoblast release osteoprotegerin (OPG) and the receptor activator of nuclear factor κB ligand (RANKL) to control the level of osteoclast differentiation and activation which in turn influences bone resorption. In this study, progressively increased, continuous tensional force was applied to PDL cells. The objective was to find out which kind of biochemical reactions occur after tensional force application and to illuminate the alveolar bone resorption and apposition mechanism. Continuous and progressively increased tensile force was applied to PDL cells cultured on a petriperm dish with a flexible membrane. The amount of PGE2 and ALP synthesis were measured after 1, 3, 6 and 12 hours of force application. Secondly, RT-PCR analysis was carried out for OPG and RANKL which control osteoclast differentiation and MMP-1, -8, -9, -13 and TIMP-1 which regulate the resolution of collagen and resorption of the osteoid layer. According to the results, we concluded that progressively increased, continuous force application to human PDL cells reduces PGE2 synthesis, and increases OPG mRNA expression.

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관련 논문AI추천

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cellsJi-Woong Kim, Jong-Hyun Nahm, Ki-Soo Lee, Yoon-Goo Kang대한치과교정학회 · 대한치과교정학회지2009 · 제39권 제4호Effect of Cortical Activation on Mechanical Force-Mediated Changes of OPG and RANKL Levels in Gingival Crevicular Fluid김정기, 이정채, 전영미, Nam-Hyun Yu, So-Yeong Kwak, So-Yeon Hong대한구강생물학회 · International Journal of Oral Biology2009 · 제34권 제4호치주인대 섬유아세포에서 Osteoprotegerin과 Osteoclast Differentiation Factor의 발현김형섭, 류성훈, 오귀옥, 허수례대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제4호Mechanisms of Osteoclastogenesis in Orthodontic Tooth Movement and Orthodontically Induced Tooth Root ResorptionNatnicha Praneetpong, Noriaki Ono, Wanida Ono, Yuta Nakai대한골대사학회 · 대한골대사학회지2023 · 제30권 제4호The effect of five osteotropic factors on osteoprotegerin mRNA expression in gingival fibroblasts고영경대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호백서의 치아이동 시 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 미치는 영향김성식, 김용덕, 박미화, 박수병, 박우경, 손우성, 전은숙대한치과교정학회 · 대한치과교정학회지2008 · 제38권 제3호

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