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Cholinergic and neurokinergic agonist-induced Ca2+ responses in rat von Ebner's gland acinar cells

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주제분류치의학

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Abstract

Von Ebner's glands (vEG) are minor salivary glands associated with circumvallate and foliate papilla. The secretions of vEG consist of microenvironment of the taste buds in the circumvallate and foliate papillae, and thus saliva from vEG plays a role in the perception of taste. The Ca2+ signaling system in rat vEG acinar cell was examined using the Ca2+-sensitive fluorescent indicator Fura-2. Agonist-induced increase in intracellular Ca2+ ([Ca2+ ]i) was stimulated by carbachol (CCh) and substance P (SP), but not by norepinephrine (NE), and recovered to control levels by their receptor antagonists dose-dependently. The effects were also observed in Ca2+-free medium, suggesting mobilization from intracellular Ca2+ store. These results in the vEG acinar cell indicate that 1) [Ca2+ ]i is at least regulated by muscarinic and neurokininergic (NK1) receptors; 2) the increases in [Ca2+]i activated by CCh and SP are mainly mediated by discharge of cytosolic calcium pool.

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