Alteration of LAR-RPTP Expression in the Rat Trigeminal Ganglion after Tooth Extraction
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LAR-RPTP (leukocyte common antigen-related receptor protein tyrosine phosphatase) is an important regulator in the nervous system, but little is known about its expression pattern in rat trigeminal ganglion (TG) neurons. To examine whether LAR-RPTP is expressed in the TG in the current study, we sacrificed rats at 0, 7, 10 and 56 day postpartum (dpp) and a second group of rats at 3 and 5 days after an experimental tooth extraction as a TG injury model. RTPCR was then used to determine the level of LAR-RPTP expression in the TG and immunohistology was employed to detect the subcellular localization of the protein. The mRNA expression of LAR-RPTP during the developmental stages in the TG was found to gradually increase. After experimental tooth extraction however, these transcript levels had significantly decreased at three days. LAR-RPTP protein signals in the TG were found to be cytoplasmic in the normal animals but interestingly, at five days after an experimental tooth extraction, these signals were rare. These results indicate that LAR-RPTP may be regulated during both the developmental as well as regenerative processes that take place in the TG. This further suggests that LAR-RPTP is not only involved in primary axonogenesis but possibly also in the molecular control of axons during TG repair.
Abstract
LAR-RPTP (leukocyte common antigen-related receptor protein tyrosine phosphatase) is an important regulator in the nervous system, but little is known about its expression pattern in rat trigeminal ganglion (TG) neurons. To examine whether LAR-RPTP is expressed in the TG in the current study, we sacrificed rats at 0, 7, 10 and 56 day postpartum (dpp) and a second group of rats at 3 and 5 days after an experimental tooth extraction as a TG injury model. RTPCR was then used to determine the level of LAR-RPTP expression in the TG and immunohistology was employed to detect the subcellular localization of the protein. The mRNA expression of LAR-RPTP during the developmental stages in the TG was found to gradually increase. After experimental tooth extraction however, these transcript levels had significantly decreased at three days. LAR-RPTP protein signals in the TG were found to be cytoplasmic in the normal animals but interestingly, at five days after an experimental tooth extraction, these signals were rare. These results indicate that LAR-RPTP may be regulated during both the developmental as well as regenerative processes that take place in the TG. This further suggests that LAR-RPTP is not only involved in primary axonogenesis but possibly also in the molecular control of axons during TG repair.
참고문헌 (25)
- Andersen JN, "Structural and evolutionary relationships among protein tyrosine phosphatase domains", Mol Cell Biol, 21, pp.7117~7136 (2001)
- Bixby JL, "Receptor tyrosine phosphatases in axon growth and guidance", NeuroReport, 11, pp.R5~R10 (2000)
- Desai CJ, "Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the “"HRP”"carbohydrate epitope", J Neurosci, 14, pp.7272~7283 (1994)
- Fried K, "Anterograde horseradish peroxidase tracing and immunohistochemistry of trigeminal ganglion tooth pulp neurons after dental nerve lesions in the rat", Neuroscience, 43, pp.269~278 (1991)
- Fried K, "Nerve injury-induced pain in the trigeminal system", Neuroscientist, 7(2), pp.155~165 (2001)
- 류효철, "GABAergic Synaptic Input to Mesencephalic Trigeminal Neurons in Rat", International Journal of Oral Biology, 30(2), pp.71~76 (2005), 대한구강생물학회
- Haworth K, "The expression of receptor tyrosine phosphatases is responsive to sciatic nerve crush", Mol Cell Neurosci, 12(3), pp.93~104 (1998)
- Marfurt CF, "The somatotopic organization of the cat trigeminal ganglion as determined by the horseradish peroxidase technique", Anat Rec, 201, pp.105~118 (1981)
- McLean J, "Enhanced rate of nerve regeneration and directional errors after sciatic nerve injury in receptor protein tyrosine phosphatase sigma knock-out mice", J Neurosci, 22, pp.5481~5491 (2002)
- Mizuno K, "Developmental regulation of gene expression for the MPTP delta isoforms in the central nervous system and the immune system", FEBS Lett, 355, pp.223~228 (1994)
- Sim MY, "Modulation of calcium currents by nociceptin", orphanin FQ in trigeminal neurons, 2003, pp.28(2), 43~47
- Schaapveld RQ, "Developmental expression of the cell adhesion molecule-like protein tyrosine phosphatases LAR, RPTPdelta and RPTPsigma in the mouse", Mech. Dev, 77, pp.59~62 (1998)
- Sessle BJ, "Acute and chronic craniofacial pain:brainstem mechanisms of nociceptive transmission and neuroplasticity, and their clinical correlates", Crit Rev Oral Biol Med, 11, pp.57~91 (2000)
- Sommer L, "RPTP delta and the novel protein tyrosine phosphatase RPTP psi are expressed in restricted regions of the developing central nervous system", Dev Dyn, 208, pp.48~61 (1997)
- Stoker AW, "Axonal localization of the CAM-like tyrosine phosphatase CRYP alpha:a signalling molecule of embryonic growth cones", Development, 121, pp.1833~1844 (1995)
- Stoker A, "Receptor tyrosine phosphatases in axon growth and guidance. Curr Opin Neurobiol. 2001;11:95-102. Sun QL, Wang J, Bookman RJ, Bixby JL. Growth cone steering by receptor tyrosine phosphatase delta defines a distinct class of guidance cue", Mol Cell Neurosci, 16, pp.686~695 (2000)
- Thompson KM, "Receptor protein tyrosine phosphatase sigma inhibits axonal regeneration and the rate of axon extension", Mol Cell Neurosci, 23, pp.681~692 (2003)
- Tian SS, "Three receptor-linked proteintyrosine phosphatases are selectively expressed on central nervous system axons in the Drosophila embryo", Cell, 67, pp.675~680 (1991)
- Van der Zee CE, "Delayed peripheral nerve regeneration and central nervous system collateral sprouting in leucocyte common antigen-related protein tyrosine phosphatase deficient mice", Eur J Neurosci, 17, pp.991~1005 (2003)
- Van Lieshout EM, "A decrease in size and number of basal forebrain cholinergic neurons is paralleled by diminished hippocampal cholinergic innervation in mice lacking leukocyte common antigen-related protein tyrosine phosphatase activity", Neuroscience, 102(4), pp.833~841 (2001)
- Van VD, "Adhesion and signaling in axonal fasciculation", Curr Opin Neurobiol, 8, pp.80~86 (1998)
- Wakisaka S, "Partial coexistence of neuropeptide Y and calbindin D28k in the trigeminal ganglion following peripheral axotomy of the inferior alveolar nerve in the rat", Brain Res, 707, pp.228~234 (1996)
- Wang J, "Receptor tyrosine phosphatase-delta is a homophilic, neurite-promoting cell adhesion molecular for CNS neurons", Mol Cell Neurosci, 14, pp.370~384 (1999)
- Xie Y, "The leukocyte common antigen-related protein tyrosine phosphatase receptor regulates regenerative neurite outgrowth in vivo", J Neurosci, 21, pp.5130~5138 (2001)
- Yan H, "A novel receptor tyrosine phosphatase sigma that is highly expressed in the nervous system", J Biol Chem, 268(24), pp.880~886 (1993)