Development of Species-specific PCR Primers for Detecting Peptoniphilus mikwangii
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In a previous study, Peptoniphilus mikwangii was isolated from the human oral cavity as a new species. The purpose of this study was to develop P. mikwangii-specific PCR primers. The PCR primers were designed, based on the nucleotide sequence of 16S ribosomal RNA (16S rDNA). The specificity of the primers was tested using genomic DNAs of 3 strains of P. mikwangii and 27 strains (27 species) of non-P. mikwangii bacteria. The sensitivity of primers sensitivity was determined using PCR, with serial dilutions of the purified genomic DNAs (4 ng to 4 fg) of P. mikwangii KCOM 1628T. The data showed that P. mikwangii-specific qPCR primers (B134-F11/B134- R1 & B134-F5/B134-R5) could detect only P. mikwangii strains, and 400 fg or 40 fg of P. mikwangii genome DNA. These results suggest that PCR primers are useful in detecting P. mikwangii from the oral cavity.
Abstract
In a previous study, Peptoniphilus mikwangii was isolated from the human oral cavity as a new species. The purpose of this study was to develop P. mikwangii-specific PCR primers. The PCR primers were designed, based on the nucleotide sequence of 16S ribosomal RNA (16S rDNA). The specificity of the primers was tested using genomic DNAs of 3 strains of P. mikwangii and 27 strains (27 species) of non-P. mikwangii bacteria. The sensitivity of primers sensitivity was determined using PCR, with serial dilutions of the purified genomic DNAs (4 ng to 4 fg) of P. mikwangii KCOM 1628T. The data showed that P. mikwangii-specific qPCR primers (B134-F11/B134- R1 & B134-F5/B134-R5) could detect only P. mikwangii strains, and 400 fg or 40 fg of P. mikwangii genome DNA. These results suggest that PCR primers are useful in detecting P. mikwangii from the oral cavity.
참고문헌 (13)
- Eugene Cho, "Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin", Current Microbiology, 70(2), pp.260~266 (2015), Springer Nature[DOI]
- Murdoch DC, "Gram-positive anaerobic cocci", Clin Microbiol Rev, 11, pp.181~120 (1998)
- Ezaki T, "Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus", Int J Syst Evol Microbiol, 51, pp.1521~1528
- Murdoch DA, "Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov", Anaerobe, 5, pp.555~559 (1999)
- Tindall BJ, "Proposal of Parvimonas gen. nov. and Quatrionicoccus gen. nov. as replacements for the illegitimate, prokaryotic, generic names Micromonas Murdoch and Shah 2000 and Quadricoccus Maszenan et al. 2002, respectively", Int J Syst Evol Microbiol, 56, pp.2711~2713 (2006)
- Seung Mi Lee, "Prevalence of Putative Periodontopathogens in Subgingival Dental Plaquesfrom Gingivitis Lesions in Korean Orthodontic Patients", The Journal of Microbiology, 43(3), pp.260~265 (2005), 한국미생물학회
- Ashimoto A, "Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions", Oral Microbiol Immunol, 11, pp.266~273 (1996)
- 이연재, "급성 치수염 및 급성 치근단 농양의 치근관으로부터의 세균 분리 및 동정", Restorative Dentistry and Endodontics, 30(5), pp.409~422 (2005), 대한치과보존학회
- 김수관, "Identification of Actinobacillus actinomycetemcomitansUsing Species-Specific 16S rDNA Primers", The Journal of Microbiology, 43(2), pp.209~212 (2005), 한국미생물학회
- 한명주, "구강내 임플랜트-지대주 연결부에서 치주질환관련 세균의 검출", 대한치과보철학회지, 46(2), pp.116~124 (2008), 대한치과보철학회
- E. Cho, "Draft Genome Sequence of the Novel Peptoniphilus sp. Strain ChDC B134, Isolated from a Human Periapical Abscess Lesion", Genome Announcements, 1(5), pp.e00822-13~e00822-13 (2013), American Society for Microbiology[DOI]
- Hwan Seon Shin, "Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 49256T", Anaerobe, 16(1), pp.43~46 (2010), Elsevier BV[DOI]
- Krieg NR, "Bergeys manual of systematic bacteriology vol. 1", pp.33~38 (2001), Springer