The Transcription Factor Mist1 Regulates the Cellular Polarity in Mouse Pancreatic Acinar Cells
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Pancreatic acinar cells exhibit a polarity that is characterized by the localization of secretory granules at the apical membrane. However, the factors that regulate cellular polarity in these cells are not well understood. In this study,we investigated the effect of Mist1, a basic helix-loop-helix transcription factor, on the cellular architecture of pancreatic acinar cells. Mist1-null mice displayed secretory granules that were diffuse throughout the pancreatic acinar cells, from the apical to basolateral membranes, whereas Mist1heterozygote mice showed apical localization of secretory granules. Deletion of the Mist1 gene decreased the expression of type 3 inositol 1,4,5-triphosphate receptors (IP3R) but did not affect apical localization and expression of IP3R2. Mist1-null mice also displayed an increase in luminal areas and an increase in the expression of zymogen granules in pancreatic acinar cells. These results suggest that Mist1 plays a critical role in polar localization of cellular organelles and in maintaining cellular architecture in mouse pancreatic acinar cells.
Abstract
Pancreatic acinar cells exhibit a polarity that is characterized by the localization of secretory granules at the apical membrane. However, the factors that regulate cellular polarity in these cells are not well understood. In this study,we investigated the effect of Mist1, a basic helix-loop-helix transcription factor, on the cellular architecture of pancreatic acinar cells. Mist1-null mice displayed secretory granules that were diffuse throughout the pancreatic acinar cells, from the apical to basolateral membranes, whereas Mist1heterozygote mice showed apical localization of secretory granules. Deletion of the Mist1 gene decreased the expression of type 3 inositol 1,4,5-triphosphate receptors (IP3R) but did not affect apical localization and expression of IP3R2. Mist1-null mice also displayed an increase in luminal areas and an increase in the expression of zymogen granules in pancreatic acinar cells. These results suggest that Mist1 plays a critical role in polar localization of cellular organelles and in maintaining cellular architecture in mouse pancreatic acinar cells.
참고문헌 (17)
- Williams JA, "Intracellular signaling mechanism activated by cholecystokinin-regulating synthesis and secretion of digestive enzymes in pancreatic acinar cells", Ann Rev Physiol, 63, pp.77~97 (2001)
- Hankard R, "Nutrition and growth in cystic fibrosis", J. Horm Res, 58(suppl 1), pp.16~20 (2002)
- Saluja AK, "Secretagogue-induced digestive enzyme activation and cell injury in rat pancreatic acini", Am J Physiol, 276(4 Pt 1), pp.G835~G842 (1999)
- Li Q, "Functional mapping of Ca2+signaling complexs in plasma membrane microdomains ofpolarized cells", J Biol Chem, 279(27), pp.27837~27840 (2004)
- Hong JH, "Scaffolding protein in Ca 2+ signaling", Int J Oral Biol, 28, pp.75~80 (2003)
- 홍정희, "Real-time Imaging of Inositol 1,4,5-trisphosphate Movement in Mouse Salivary Gland Cells", International Journal of Oral Biology, 33(4), pp.125~129 (2008), 대한구강생물학회
- Meldolesi J, "Membrane and membrane surfaces. Dynamics of cytoplasmic membranes in pancreatic acinar cells", Philos Trans R Soc Lond B Biol Sci, 268(891), pp.39~53 (1974)
- 양동기, "MOLECULAR MECHANISM OF PANCREATIC AND SALIVARY GLAND FLUID AND HCO3- SECRETION", PHYSIOLOGICAL REVIEWS, 92(1), pp.39~74, AMER PHYSIOLOGICAL SOC
- Krapp A, "The p48 subunit of transcription factor PTF1 is a new exocrine pancreas-specific basic helixloop- helix protein", EMBO J, 15(16), pp.4317~4329 (1996)
- Lemercier C, "Mist1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern", Dev Biol, 182(1), pp.101~113 (1997)
- Molkentin JD, "Defining the regulatory networks for muscle development", Curr Opin Genet Dev, 6(4), pp.445~453 (1996)
- Krapp A, "The bHLH protein PTF1-p48 is essential for the formation of the exocrine and the correct spatial organization of the endocrine pancreas", Genes Dev, 12(23), pp.3752~3763 (1998)
- Pin CL, "Mist1 expression is a common link among serous exocrine cells exhibiting regulated exocytosis", Anat Rec, 259(2), pp.157~167 (2000)
- 신동민, "Aberrant localization of intracellular organelles, Ca2+ Signaling, and Exocytosis in Mist1 Null Mice", JOURNAL OF BIOLOGICAL CHEMISTRY, 280(13), pp.12668~12675, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- 신동민, "The mammalian Sec6", 8 complex interacts with Ca2+ signaling complexes and regulates their activity, 200009, pp.150(5), 1101~1112[DOI]
- i waves in polarized exocrine cells, "JOURNAL OF BIOLOGICAL CHEMISTRY", 200111, 44146~44156, pp.AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Pin CL, "The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity", J Cell Biol, 155(4), pp.519~530 (2001)