Extracellular Matrix Metalloproteinase Inducer is Regulated Developmentally and Functionally in the Rat Submandibular Gland
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The salivary gland undergoes complex process of growth and differentiation of the branching morphogenesis of ductal system during the prenatal and early postnatal periods which are regulated by various elements in the extracellular matrix. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a cell adhesion molecule. In the present study, localization and expression of EMMPRIN in development and effects of chorda-lingual denervation and cyclosporine A (CsA) treatment on the EMMPRIN expression were investigated. Immunohistochemistry, RT-PCR and Western blot were used to determine expression level. Immunohistochemistry revealed that EMMPRIN was localized specifically in the cytoplasm of ductal cells, not acini of the submandibular gland all the postnatal periods. At prenatal day 18, when the formation of ducts was not definite, no immunoreactivity was observed. Both Western blot and RT-PCR analyses revealed that EMMPRIN expression was maintained up to postnatal day 7, decreased after postnatal day 10. The EMMPRIN expression was upregulated by the surgical denervation of the chorda-lingual nerve in the gland as well as by the CsA treatment. The present study suggests that EMMPRIN is a crucial molecule for maintaining physiological functions of the salivary gland.
Abstract
The salivary gland undergoes complex process of growth and differentiation of the branching morphogenesis of ductal system during the prenatal and early postnatal periods which are regulated by various elements in the extracellular matrix. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a cell adhesion molecule. In the present study, localization and expression of EMMPRIN in development and effects of chorda-lingual denervation and cyclosporine A (CsA) treatment on the EMMPRIN expression were investigated. Immunohistochemistry, RT-PCR and Western blot were used to determine expression level. Immunohistochemistry revealed that EMMPRIN was localized specifically in the cytoplasm of ductal cells, not acini of the submandibular gland all the postnatal periods. At prenatal day 18, when the formation of ducts was not definite, no immunoreactivity was observed. Both Western blot and RT-PCR analyses revealed that EMMPRIN expression was maintained up to postnatal day 7, decreased after postnatal day 10. The EMMPRIN expression was upregulated by the surgical denervation of the chorda-lingual nerve in the gland as well as by the CsA treatment. The present study suggests that EMMPRIN is a crucial molecule for maintaining physiological functions of the salivary gland.
참고문헌 (26)
- Denny PC, "Salivary glands : a paradigm for diversity of gland development", Crit Rev Oral Biol Med, 8, pp.51~75 (1997)
- Gresik EW, "Hormonal regulation of epidermal growth factor and protease in the submandibular gland of the adult mouse", Endocrinology, 109, pp.924~929 (1981)
- Chang JC, "Canalicular blockage", Aust J Ophthamol, 1, pp.58~62 (1973)
- Gresik EW, "Postnatal developmental changes in submandibular glands of rats and mice", J Histochem Cytochem, 28, pp.860~870 (1980)
- Yurchenko V, "CD147 is a signaling receptor for cyclophilin B", Biochem Biophys Res Commun, 288, pp.786~788 (2001)
- Igakura T, "A null mutation in basigin, an immunoglobulin superfamily member, indicates its important roles in peri-implantation development and spermatogenesis", Dev Biol, 194, pp.152~165 (1998)
- Sun J, "Regulation of MMP-1 and MMP-2production through CD147", extracellular matrix metalloproteinase inducer interactions, 2001, pp.61, 2276~2281
- Norgauer J, "Elevated expression of extracellular matrix metalloproteinase inducer(CD147)and membrane-type matrix metalloproteinases in venous leg ulcers", Br J Dermatol, 147, pp.1180~1186 (2002)
- Songlin Piao, "Increased expression of CD147 and MMP-9 is correlated with poor prognosis of salivary duct carcinoma", Journal of Cancer Research and Clinical Oncology, 138(4), pp.627~635 (2012), Springer Nature[DOI]
- Yang X, "EMMPRIN contributes to the in vitro invasion of human salivary adenoid cystic carcinoma cells", Oncology Reports (2011), Spandidos Publications[DOI]
- Fernandes RP, "Differential expression of proliferative, cytoskeletal, and adhesive proteins during postnatal development of the hamster submandibular gland", Histochem Cell Biol, 111, pp.153~162 (1999)
- Borghese E, "The development in vitro of the submandibular and sublingual glands of Mus musculus", J Anat, 84, pp.287~302 (1950)
- Jacoby F, "Cyclosporin A : a powerful immunosuppressant", Can Med Assoc J, 126, pp.1041~1046 (1982)
- Yang X, "EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo", Cancer Biol Ther, 13, pp.85~91 (2012)
- Piao S, "Increased expression of CD147 and MMP-9 is correlated with poor prognosis of salivary duct carcinoma", J Cancer Res Clin Oncol, 138, pp.627~635 (2012)
- Fahr A, "Cyclosporin clinical pharmacokinetics", Clin Pharmacokinet, 24, pp.472~495 (1993)
- Wenger RM., "Pharmacology of cyclosporin (sandimmune). II. Chemistry", Pharmacol Rev, 41, pp.243~247 (1990)
- Galat A, "Peptidylproline cis-trans-isomerases : immunophilins", Eur J Biochem, 216, pp.689~707 (1993)
- Yurchenko V, "Active site residues of cyclophilin A are crucial for its signaling activity via CD147", J Biol Chem, 277, pp.22959~22965 (2002)
- Dardenne M, "Stimulatory effects of cyclosporin A on human and mouse thymic epithelial cells", Eur J Immunol, 1792, pp.275~279 (1987)
- Dehpour AR, "Cyclosporin A-induced functional and morphological changes in pilocarpine treated rat submandibular glands", Toxicology, 108, pp.65~71 (1996)
- Malesevic M, "Anti-inflammatory effects of extracellular cyclosporins are exclusively mediated by CD147", J Med Chem, 56, pp.7302~7311 (2013)
- Chiu R, "Effects of altered expression and localization of Cyclophilin A on differentiation of P19 embryonic carcinoma cells", Cell Mol Neurobiol, 23, pp.929~943 (2003)
- Song J, "Cyclophilin A is required for retinoic acid-induced neuronal differentiation in p19 cells", J Biol Chem, 279, pp.24414~24419 (2004)
- Avramut M, "Immunophilins in nervous system degeneration and regeneration", Curr Top Med Chem, 3, pp.1376~1382 (2003)
- Xinjie Yang, "Expression of EMMPRIN in adenoid cystic carcinoma of salivary glands: Correlation with tumor progression and patients’ prognosis", Oral Oncology, 46(10), pp.755~760 (2010), Elsevier BV[DOI]