톳을 이용한 기능성 다당류 추출 및 항비만 효과 검출
Enzymatic Extraction of Polysaccharide from Hijikia fusiforme and its Effects on Anti-obesity
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Hizikia fusiforme, a brown algae, is a major polysaccharide composed of alginate and fucoidan. In this study, the extraction of the useful substance using the organic solvent is generally avoided and a simple biocompatible / eco-friendly extraction method is devised to obtain an extract containing the water-soluble polymer polysaccharide as the main component from the dried or raw Hizikia fusiforme. In this study, the water soluble polymer polysaccharides were prepared with enzyme digestion and recovered with ethanol precipitation. The changes of reducing sugar content and protein content were observed and the molecular weight distribution was analyzed after enzyme digestion. Molecular weight measurements were performed using HPAEC and HPLC to reveal the constituents of the fraction material having anti-obesity bioactivity. The molecular weight (MW) of the extract was analyzed using a RI (Refractive Index) detector using a GPC column (PSS Suprema 1,000Å). Dextran (MW: 5, 12, 50, 150, 670 kDa) was used as a standard. As a result, polysaccharides composed of fucose, galactose and xylose (Average molecular weight: 18.5 kD) were identified. The IC50 value was determined using an enzyme extract having the best inhibition rate in the anti-obesity activity inhibition experiment. viscozyme, celluclast and alcalse were 14.06, 11.27, and 10.02 mg/ml, respectively, indicating that alcalase is most suitable.
Abstract
Hizikia fusiforme, a brown algae, is a major polysaccharide composed of alginate and fucoidan. In this study, the extraction of the useful substance using the organic solvent is generally avoided and a simple biocompatible / eco-friendly extraction method is devised to obtain an extract containing the water-soluble polymer polysaccharide as the main component from the dried or raw Hizikia fusiforme. In this study, the water soluble polymer polysaccharides were prepared with enzyme digestion and recovered with ethanol precipitation. The changes of reducing sugar content and protein content were observed and the molecular weight distribution was analyzed after enzyme digestion. Molecular weight measurements were performed using HPAEC and HPLC to reveal the constituents of the fraction material having anti-obesity bioactivity. The molecular weight (MW) of the extract was analyzed using a RI (Refractive Index) detector using a GPC column (PSS Suprema 1,000Å). Dextran (MW: 5, 12, 50, 150, 670 kDa) was used as a standard. As a result, polysaccharides composed of fucose, galactose and xylose (Average molecular weight: 18.5 kD) were identified. The IC50 value was determined using an enzyme extract having the best inhibition rate in the anti-obesity activity inhibition experiment. viscozyme, celluclast and alcalse were 14.06, 11.27, and 10.02 mg/ml, respectively, indicating that alcalase is most suitable.
참고문헌 (22)
- Kolsi, R. B. A., , International Journal of Biological Macromolecules, 102, pp.119~129 (2017)
- Jeongmin Lee, "Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models", Nutrition Research and Practice, 10(3), pp.265~273 (2016), 한국영양학회
- Kwon, T. H., , Journal of the Science of Food and Agriculture, 95(9), pp.1830~1837 (2015)
- Castro, M., , Current Topics in Medicinal Chemistry, 16(23), pp.2577~2604 (2016)
- Ding, Y., , Marine Drugs, 17(11), pp.637~ (2019)
- Kim, I. H., , International Journal of Molecular Medicine, 41(2), pp.924~934 (2018)
- Zhao, B., , Plos One, 14(6) (2019)
- Kang, M. C., , Food and Chemical Toxicology, 90, pp.36~44 (2016)
- 김꽃봉우리, "Lipase Inhibitory Activity of Ethyl Acetate Fraction from Ecklonia cava Extracts", Biotechnology and Bioprocess Engineering, 17(4), pp.739~745 (2012), 한국생물공학회
- Kawee-Ai, A., , Journal of Oceanology and Limnology, 37(3), pp.928~937 (2019)
- Maeda, H., , Molecular Medicine Reports, 2(6), pp.897~902 (2009)
- Pandey, S., , Microbial Cell Factories, 12(1), pp.35~ (2013)
- Anand, N., , Plant Genetic Resources, 14(4), pp.303~313 (2016)
- Cho, S. H., , The FASEB Journal, 22, pp.681~681 (2008)
- Hassan, M. M., , Journal of Applied Polymer Science, 120(3), pp.1843~1849 (2011)
- Rai, S. N., , Indian Journal of Animal Sciences, 78(11), pp.1278~1280 (2008)
- Shimoda, Y., , Epidemiology, 20(6), pp.124~125 (2009)
- Solomon, R. J., , World Journal of Microbiology & Biotechnology, 24(9), pp.1747~1752 (2008)
- Thomas, N. V., , Environmental Toxicology and Pharmacology, 32(3), pp.325~335 (2011)
- Chakraborty, S., , Journal of Carbohydrate Chemistry, 36(1), pp.1~19 (2017)
- Charles, A. L., , Food and Chemical Toxicology, 45(12), pp.2390~2396 (2007)
- Hu, X., , Journal of Functional Foods, 21, pp.372~387 (2016)