LED (Light-Emitting Diode)를 이용한 미세조류 (Chaetoceros calcitrans)의 성장 및 생화학적 조성 변화
Changes in the growth and biochemical composition of Chaetoceros calcitrans cultures using light-emitting diodes
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초록
The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR≧CON>LB≧LW≧LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures (6.3㎍/mL), whereas LW showed the lowest (3.6㎍/mL; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.
Abstract
The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR≧CON>LB≧LW≧LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures (6.3㎍/mL), whereas LW showed the lowest (3.6㎍/mL; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.
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