Activated Leukocyte Cell Adhesion Molecule: Expression in the Uterine Endometrium during the Estrous Cycle and Pregnancy in Pigs
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The pig exhibits true epitheliochorial placentation, where the fetal membrane maintains attachment throughout pregnancy but does not invade into the maternal uterine endometrium. Accordingly, the expression and function of cell adhesion molecules are very important for embryo implantation and the establishment of pregnancy. In our recent microarray analysis, we found that activated leukocyte cell adhesion molecule (ALCAM) was expressed in the uterine endometrium during pregnancy in pigs. To better understand the roles of ALCAM in the establishment and maintenance of pregnancy, we examined ALCAM expression in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that ALCAM was differentially expressed in the uterine endometrium during the estrous cycle and pregnancy, with the highest levels on D12 of pregnancy. ALCAM mRNA was localized to the luminal and glandular epithelial cells and to the trophectoderm of conceptuses during early pregnancy. The steroid hormones estrogen and progesterone had no effect on ALCAM expression in an endometrial explant culture study. Further, we found that ALCAM expression in the uterine endometrium from gilts with somatic cell nuclear transfer-derived embryos was not different from that in gilts with embryos from natural mating. ALCAM was expressed in a pregnancy stage- and cell type-specific manner in the uterine endometrium and conceptuses during pregnancy. These findings suggest that ALCAM may play a role in the establishment of pregnancy. Further analysis of ALCAM will provide insight into the implantation process and establishment of pregnancy in pigs.
Abstract
The pig exhibits true epitheliochorial placentation, where the fetal membrane maintains attachment throughout pregnancy but does not invade into the maternal uterine endometrium. Accordingly, the expression and function of cell adhesion molecules are very important for embryo implantation and the establishment of pregnancy. In our recent microarray analysis, we found that activated leukocyte cell adhesion molecule (ALCAM) was expressed in the uterine endometrium during pregnancy in pigs. To better understand the roles of ALCAM in the establishment and maintenance of pregnancy, we examined ALCAM expression in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that ALCAM was differentially expressed in the uterine endometrium during the estrous cycle and pregnancy, with the highest levels on D12 of pregnancy. ALCAM mRNA was localized to the luminal and glandular epithelial cells and to the trophectoderm of conceptuses during early pregnancy. The steroid hormones estrogen and progesterone had no effect on ALCAM expression in an endometrial explant culture study. Further, we found that ALCAM expression in the uterine endometrium from gilts with somatic cell nuclear transfer-derived embryos was not different from that in gilts with embryos from natural mating. ALCAM was expressed in a pregnancy stage- and cell type-specific manner in the uterine endometrium and conceptuses during pregnancy. These findings suggest that ALCAM may play a role in the establishment of pregnancy. Further analysis of ALCAM will provide insight into the implantation process and establishment of pregnancy in pigs.
참고문헌 (46)
- Arai, F, "Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation", J. Exp. Med, 195(12), pp.1549~1563 (2002)
- Ashworth, M. D, "Endometrial gene expression of acute phase extracellular matrix components following estrogen disruption of pregnancy in pigs", Anim. Reprod. Sci, 122, pp.215~221 (2010)
- Bauersachs, S, "The endometrium responds differently to cloned versus fertilized embryos", Proc. Natl. Acad. Sci. USA, 106(14), pp.5681~5686 (2009)
- 플러더블유베이저, "Comparative aspects of implantation", REPRODUCTION, 138(2), pp.195~209, BIO SCIENTIFICA LTD
- Bowen, J. A, "Spatial and temporal analyses of integrin and Muc-1 expression in porcine uterine epithelium and trophectoderm in vivo", Biol. Reprod, 55(5), pp.1098~1106 (1996)
- Bowen, M. A, "Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand", J. Exp. Med, 181(6), pp.2213~2220 (1995)
- Braissant, O, "A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mRNAs on tissue sections", Biochemica, 1, pp.10~16 (1998)
- Bruder, S. P, "Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation", J. Bone Miner. Res, 13(4), pp.655~663 (1998)
- Burghardt, R. C, "Extracellular matrix and the implantation cascade in pigs", J. Reprod. Fertil. Suppl, 52, pp.151~164 (1997)
- Burghardt, R. C, "Integrins and extracellular matrix proteins at the maternal-fetal interface in domestic animals", Cells, Tissues, Organs, 172(3), pp.202~217 (2002)
- Campbell, K. H. S., "Cloning: Eight years after Dolly", Reprod. Domest. Anim, 40(4), pp.256~268 (2005)
- Cayrol, R, "Activated leukocyte cell adhesion molecule promotes leukocyte trafficking into the central nervous system", Nat. Immunol, 9(2), pp.137~145 (2008)
- Chae, J. I, "Proteomic analysis of pancreas derive from adult cloned pig", Biochem. Biophys. Res. Commun, 366(2), pp.379~387 (2008)
- Cortes, F, "Differential expression of KDR", VEGFR-2 and CD34 during mesoderm development of the early human embryo, 1999, pp.83, 161~164
- Dantzer, V, "Electron microscopy of the initial stages of placentation in the pig", Anat. Embryol, 172(3), pp.281~293 (1985)
- Erikson, D. W, "Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration", Biol. Reprod, 81(5), pp.814~825 (2009)
- Farin, P. W., "Errors in development of fetuses and placentas from in vitro-produced bovine embryos", Theriogenology, 65(1), pp.178~191 (2006)
- Fernando, S. C, "Porcine kallikrein gene family: genomic structure, mapping, and differential expression analysis", Genomics, 89(3), pp.429~438 (2007)
- Fujiwara, H, "Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM", CD166), 2003, pp.88(7), 3437~3443
- Geisert, R. D, "Regulation of conceptus development and attachment in pigs", J. Reprod. Fertil. Suppl, 52, pp.133~149 (1997)
- Gessert, S., "DM-GRASP", ALCAM, Dev. Biol, pp.2008, 321(1)[DOI]
- Hagen, D. R, "Development of one-cell porcine embryos to the blastocyst stage in simple media", J. Anim. Sci, 69(3), pp.1147~1150 (1991)
- Hernandez-Gonzalez, I, "Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune-related genes: does this expand their role in the ovulation process", Mol. Endocrinol, 20(6), pp.1300~1321 (2006)
- Humpherys, D, "Epigenetic instability in ES cells and cloned mice", Science, 293(5527), pp.95~97 (2001)
- Hyun, S. H, "Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos", Theriogenology, 59(7), pp.1641~1649 (2003)
- Ikeda, K, "Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function", J. Biol. Chem, 279(53), pp.55315~55323 (2004)
- Jezierska, A, "ALCAM", CD166 protects breast cancer cells against apoptosis and autophagy, 2006, pp.12(8), 263~273
- Ka, H., "Keratinocyte growth factor is up-regulated by estrogen in the porcine uterine endometrium and functions in trophectoderm cell proliferation and differentiation", Endocrinology, 142(6), pp.2303~2310 (2001)
- Ka, H, "Gene expression profiling of the uterus with embryos cloned by somatic cell nuclear transfer on day 30 of pregnancy", Anim. Reprod. Sci, 108, pp.79~91 (2008)
- Ka, H, "Keratinocyte growth factor: expression by endometrial epithelia of the porcine uterus", Biol. Reprod, 62(6), pp.1772~1778 (2000)
- Keefer, C. L, "Lessons learned from nuclear transfer (cloning)", Theriogenology, 69(1), pp.48~54 (2008)
- Kim, M., "Aberrant expression of retinol-binding protein, osteopontin and fibroblast growth factor 7 in the porcine uterine endometrium of pregnant recipients carrying embryos produced by somatic cell nuclear transfer", Anim. Reprod. Sci, 112, pp.172~181 (2009)
- Konno, A., "Tissue distribution of CD6 and CD6 ligand in cattle: expression of the CD6 ligand (CD166) in the autonomic nervous system of cattle and the human", J. Leukoc. Biol, 69(6), pp.944~950 (2001)
- Lefevre, F, "Interferon-gamma gene and protein are spontaneously expressed by the porcine trophectoderm early in gestation", Eur. J. Immunol, 20(11), pp.2485~2490 (1990)
- Levesque, M. C, "Cytokine-regulated expression of activated leukocyte cell adhesion molecule (CD166) on monocyte-lineage cells and in rheumatoid arthritis synovium", Arthritis Rheum, 41(12), pp.2221~2229 (1998)
- Livak, K. J, "Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method", Methods, 25(4), pp.402~408 (2001)
- Masedunskas, A., "Activated leukocyte cell adhesion molecule is a component of the endothelial junction involved in transendothelial monocyte migration", FEBS Lett, 580(11), pp.2637~2745 (2006)
- Ohneda, O, "ALCAM (CD166): its role in hematopoietic and endothelial development", Blood, 98(7), pp.2134~2142 (2001)
- Patel, D. D, "Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells", J. Exp. Med, 181(4), pp.1563~1568 (1995)
- Petters, R. M, "Culture of pig embryos", J. Reprod. Fertil. Suppl, 48, pp.61~73 (1993)
- Pope, W, "Embryonic mortality in swine In Embryonic mortality in domestic species", pp.53~78 (1994), CRC Press
- Ross, J. W., "Characterization of the interleukin-1beta system during porcine trophoblastic elongation and early placental attachment", Biol. Reprod, 69(4), pp.1251~1259 (2003)
- Singer, N. G, "CD6 dependent interactions of T cells and keratinocytes: functional evidence for a second CD6 ligand on gamma-interferon activated keratinocytes", Immunol. Lett, 58(1), pp.9~14 (1997)
- Song, G, "Cathepsin B, cathepsin L, and cystatin C in the porcine uterus and placenta: potential roles in endometrial", placental remodeling and in fluid-phase transport of proteins secreted by uterine epithelia across placental areolae, 2010, pp.82(5), 854~864
- Stephan, J. P, "Distribution and function of the adhesion molecule BEN during rat development", Dev. Biol, 212(2), pp.264~277 (1999)
- Uchida, N., "The characterization, molecular cloning, and expression of a novel hematopoietic cell antigen from CD34+ human bone marrow cells", Blood, 89(8), pp.2706~2716 (1997)