Effects of Capsaicin on Adipogenic Differentiation in Bovine Bone Marrow Mesenchymal Stem Cell
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Capsaicin is a major constituent of hot chili peppers that influences lipid metabolism in animals. In this study, we explored the effects of capsaicin on adipogenic differentiation of bovine bone marrow mesenchymal stem cells (BMSCs) in a dose- and time-dependent manner. The BMSCs were treated with various concentrations of capsaicin (0, 0.1, 1, 5, and 10 μM) for 2, 4, and 6 days. Capsaicin suppressed fat deposition significantly during adipogenic differentiation. Peroxisome proliferator-activated receptor gamma, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer binding protein alpha, fatty acid binding protein 4, and stearoyl-CoA desaturase expression decreased after capsaicin treatment. We showed that the number of apoptotic cells increased in dose- and time-dependent manners. Furthermore, we found that capsaicin increased the expression levels of apoptotic genes, such as B-cell lymphoma 2-associated X protein and caspase 3. Overall, capsaicin inhibits fat deposition by triggering apoptosis.
Abstract
Capsaicin is a major constituent of hot chili peppers that influences lipid metabolism in animals. In this study, we explored the effects of capsaicin on adipogenic differentiation of bovine bone marrow mesenchymal stem cells (BMSCs) in a dose- and time-dependent manner. The BMSCs were treated with various concentrations of capsaicin (0, 0.1, 1, 5, and 10 μM) for 2, 4, and 6 days. Capsaicin suppressed fat deposition significantly during adipogenic differentiation. Peroxisome proliferator-activated receptor gamma, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer binding protein alpha, fatty acid binding protein 4, and stearoyl-CoA desaturase expression decreased after capsaicin treatment. We showed that the number of apoptotic cells increased in dose- and time-dependent manners. Furthermore, we found that capsaicin increased the expression levels of apoptotic genes, such as B-cell lymphoma 2-associated X protein and caspase 3. Overall, capsaicin inhibits fat deposition by triggering apoptosis.
참고문헌 (37)
- Anandakumar, P., "Capsaicin provokes apoptosis and restricts benzo(a)pyrene induced lung tumorigenesis in Swiss albino mice", Int. Immunopharmacol, 17, pp.254~259 (2013)
- Beswick, N. S., "Influence of bovine growth hormone and growth hormone–releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl– CoA desaturase in the bovine mammary gland and adipose tissue", J. Anim. Sci, 78, pp.412~419 (2000)
- Bitencourt, S., "Capsaicin induces de–differentiation of activated hepatic stellate cell", Biochem. Cell Biol, 90, pp.683~690 (2012)
- Chakraborty, S., "Restoration of p53", miR-34a regulatory axis decreases survival advantage and ensures Bax-dependent apoptosis of non-small cell lung carcinoma cells, 2014, pp.588, 549~559
- Chang, H. Y., "Proteases for cell suicide: functions and regulation of caspases", Microbiol. Mol. Biol. Rev, 64, pp.821~846 (2000)
- Deans, R. J., "Mesenchymal stem cells: biology and potential clinical uses", Exp. Hematol, 28, pp.875~884 (2000)
- Guimarães, E. L. M., "Hepatic stellate cell line modulates lipogenic transcription factors", Liver Int, 27, pp.1255~1264 (2007)
- Haramizu, S., "Capsinoids, non-pungent capsaicin analogs, reduce body fat accumulation without weight rebound unlike dietary restriction in mice", Biosci. Biotechnol. Biochem, 75, pp.95~99 (2011)
- Hsu, C. L., "Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells", J. Agric. Food Chem, 55, pp.1730~1736 (2007)
- Hwang, J. T., "Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMPactivated protein kinase", Biochem. Biophys. Res. Commun, 338, pp.694~699 (2005)
- Jeong, J., "Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls", J. Anim. Sci, 91, pp.4692~4704 (2013)
- Jeong, J., "Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers", J. Anim. Sci, 90, pp.2044~2053 (2012)
- Kennedy, A., "Antiobesity mechanisms of action of conjugated linoleic acid", J. Nutr. Biochem, 21, pp.171~179 (2010)
- Rina Yu, "Capsaicin, a Spicy Component of Hot Pepper, Induces Apoptosis by Activation of the Peroxisome Proliferator-Activated receptor gamma in HT-29 human colon cancer cells", Journal of Medicinal Food, 7(3), pp.267~273 (2004), 한국식품영양과학회
- Lee, M. S., "Effects of capsaicin on lipid catabolism in 3T3-L1 adipocytes", Phytother. Res, 25, pp.935~939 (2011)
- Lin, C. H, "Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells", BMC Complement. Altern. Med, 13, pp.46~ (2013)
- Moisá, S. J., "Bioinformatics analysis of transcriptome dynamics during growth in angus cattle longissimus muscle", Bioinform. Biol. Insights, 7, pp.253~270 (2013)
- Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- Pande, S., "Potentiation of hypolipidemic and weight–reducing influence of dietary tender cluster bean (Cyamopsis tetragonoloba) when combined with capsaicin in high-fat-fed rats", J. Agric. Food Chem, 60, pp.8155~8162 (2012)
- Park, S. Y., "Capsaicin induces apoptosis and modulates MAPK signaling in human gastric cancer cells", Mol. Med. Rep, 9, pp.499~502 (2014)
- Pittenger, M. F., "Multilineage potential of adult human mesenchymal stem cells", Science, 284, pp.143~147 (1999)
- Ringe, J, "Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages", Cell Tissue Res, 307, pp.321~327 (2002)
- Shin, D. W., "(–)–Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPAR gamma transactivation", Biochem. Pharmacol, 77, pp.125~133 (2009)
- Simmons, D. J., "Partial characterization of rat marrow stromal cells", Calcif. Tissue Int, 48, pp.326~334 (1991)
- Skrzypski, M, "Capsaicin induces cytotoxicity in pancreatic neuroendocrine tumor cells via mitochondrial action", Cell. Signal, 26, pp.41~48 (2014)
- Tang, Q. Q., "Adipogenesis: From stem cell to adipocyte", Annu. Rev. Biochem, 81, pp.715~736 (2012)
- Prockop, D. J., "Marrow stromal cells as stem cells for nonhematopoietic tissues", Science, 276, pp.71~74 (1997)
- Tsukamoto, H., "Anti-adipogenic regulation underlies hepatic stellate cell transdifferentiation", J. Gastroenterol. Hepatol, 21(Suppl 3), pp.S102~S105 (2006)
- Vermes, I., "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V", J. Immunol. Methods, 184, pp.39~51 (1995)
- Wang, C. Z, "PPARγ agonist suppresses TLR4 expression and TNF-αproduction in LPS stimulated monocyte leukemia cells", Cell Biochem. Biophys, 60, pp.167~172 (2011)
- Wang, Y. W., "Conjugated linoleic acid and obesity control: efficacy and mechanisms", Int. J. Obes. Relat. Metab. Disord, 28, pp.941~955 (2004)
- Wise, L. S., "Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3 T 3 cells", J. Biol. Chem, 254, pp.273~275 (1979)
- Woodbury, D., "Adult rat and human bone marrow stromal cells differentiate into neurons", J. Neurosci. Res, 61, pp.364~370 (2000)
- Zhang, L. L., "PPARγ attenuates intimal hyperplasia by inhibiting TLR4-mediated inflammation in vascular smooth muscle cells", Cardiovasc. Res, 92, pp.484~493 (2011)
- Zhang, L. L., "Activation of transient receptor potential vanilloid type-1 channel prevents adipogenesis and obesity", Circ. Res, 100, pp.1063~1070 (2007)
- Zhang, Y., "Hsa-let-7g miRNA targets caspase-3 and inhibits the apoptosis induced by ox-LDL in endothelial cells", Int. J. Mol. Sci, 14, pp.22708~22720 (2013)
- Zuk, P. A, "Multilineage cells from human adipose tissue: Implications for cell-based therapies", Tissue Eng, 7, pp.211~228 (2001)
