Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
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The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (–) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB– oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.
Abstract
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (–) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB– oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.
참고문헌 (27)
- Alm, H., "Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity", Theriogenology, 63, pp.2194~2205 (2005)
- Anguita, B., "Effect of the apoptosis rate observed in oocytes and cumulus cells on embryo development in prepubertal goats", Anim. Reprod. Sci, 116, pp.95~106 (2009)
- Bhojwani, S., "Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer", Theriogenology, 67, pp.341~345 (2007)
- Dai, X., "Somatic nucleus reprogramming is significantly improved by m-carboxycinnamic acid bishydroxamide, a histone deacetylase inhibitor", J. Biol. Chem, 285, pp.31002~31010 (2010)
- Das, Z. C., "Increasing histone acetylation of cloned embryos, but not donor cells, by sodium butyrate improves their in vitro development in pigs", Cell. Reprogram, 12, pp.95~104 (2010)
- Ericsson, S. A., "Assessment of porcine oocytes using brilliant cresyl blue", Theriogenology, 39, pp.214~ (1993)
- Guthrie, H. D., "Changes in porcine oocyte germinal vesicle development as follicles approach preovulatory maturity", Theriogenology, 54, pp.389~399 (2000)
- Ishizaki, C., "Developmental competence of porcine oocytes selected by brilliant cresyl blue and matured individually in a chemically defined culture medium", Theriogenology, 72, pp.72~80 (2009)
- Kempisty, B., "Zona pellucida glycoprotein 3(pZP3)and integrin beta2(ITGB2)mRNA and protein expression in porcine oocytes after single and double exposure to brilliant cresyl blue test", Theriogenology, 75, pp.1525~1535 (2011)
- Mirshamsi, S. M., "Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle", Anim. Reprod. Sci, 136, pp.245~251 (2013)
- Opiela, J., "The utility of brilliant cresyl blue (BCB) staining of mammalian oocytes used for in vitro embryo production (IVP)", Reprod. Biol, 13, pp.177~183 (2013)
- Opiela, J., "Interactions among activity of glucose-6-phosphate dehydrogenase in immature oocytes, expression of apoptosis-related genes Bcl-2 and Bax, and developmental competence following IVP in cattle", Theriogenology, 69, pp.546~555 (2008)
- Pawlak, P., "Disturbances of nuclear maturation in BCB positive oocytes collected from peri-pubertal gilts", Theriogenology, 75, pp.832~840 (2011)
- Pawlak, P., "The quality of porcine oocytes is affected by sexual maturity of the donor gilt", Reprod. Biol, 11, pp.1~18 (2011)
- Pawlak, P., "Differences in cytoplasmic maturation between the BCB+ and control porcine oocytes do not justify application of the BCB test for a standard IVM protocol", J. Reprod. Dev, 60, pp.28~36 (2014)
- Pereira, G. R., "Selection of developmentally competent immature equine oocytes with brilliant cresyl blue stain prior to in vitro maturation with equine growth hormone", Zygote, 22, pp.500~504 (2014)
- Pujol, M., "Developmental competence of heifer oocytes selected using the brilliant cresyl blue(BCB)test", Theriogenology, 61, pp.735~744 (2004)
- Rodrigues, B. A., "Preliminary study in immature canine oocytes stained with brilliant cresyl blue and obtained from bitches with low and high progesterone serum profiles", Reprod. Domest. Anim, 44(2), pp.255~258 (2009)
- Rodríguez-González, E., "Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation", Reprod. Nutr. Dev, 43, pp.179~187 (2003)
- Rodríguez-González, E., "Selection of prepubertal goat oocytes using the brilliant cresyl blue test", Theriogenology, 57, pp.1397~1409 (2002)
- Silva, D. S., "Selection of bovine oocytes by brilliant cresyl blue staining : effect on meiosis progression, organelle distribution and embryo development", Zygote, 21, pp.250~255 (2013)
- Su, J., "Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo development of SCNT embryos in cattle", PloS one, 7, pp.e36181~ (2012)
- Sun, Q. Y., "Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro", Reproduction, 122, pp.155~163 (2001)
- Sun, X. S., "Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes", Mol. Reprod. Dev, 69, pp.228~234 (2004)
- Tiffin, G. J., "Glucose and glutamine metabolism in preattachment cattle embryos in relation to sex and stage of development", J. Reprod. Fertil, 93, pp.125~132 (1991)
- Wu, Y. G., "Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: A study using the mouse model", Cell Res, 17, pp.722~731 (2007)
- Yang, C. R., "Short-term preservation of porcine oocytes in ambient temperature: novel approaches", PloS one, 5, pp.e14242~ (2010)