Effect of L- or DL-methionine Supplementation on Nitrogen Retention, Serum Amino Acid Concentrations and Blood Metabolites Profile in Starter Pigs
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The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DLmethionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred (Duroc×Landrace×Yorkshire) barrows weighing 15.45±0.88 kg were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig’s requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p<0.01). The N retention of pigs fed diets supplemented with the same inclusion levels of DL-Met or L-Met were not different (p>0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs.
Abstract
The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DLmethionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred (Duroc×Landrace×Yorkshire) barrows weighing 15.45±0.88 kg were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig’s requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p<0.01). The N retention of pigs fed diets supplemented with the same inclusion levels of DL-Met or L-Met were not different (p>0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs.
참고문헌 (32)
- AOAC, "Official Methods of Analysis", Association of Official Analytical Chemists, ~ (2007)
- Ball, R. O., "The in vivo sparing of methionine by cysteine in sulfur amino acid requirements in animal models and adult humans", J. Nutr, 136, pp.1682S~1693S (2006)
- Brown, J. A, "Urea excretion in the pig: an indicator of protein quality and amino acid requirements", J. Nutr, 104, pp.542~545 (1974)
- Chen, Y., "L-Methionine supplementation maintains the integrity and barrier function of the small-intestinal mucosa in post-weaning piglets", Amino Acids, 46, pp.1131~1142 (2014)
- Cho, E. S, "D-methionine utilization in young miniature pigs, adult rabbits, and adult dogs", J. Parenter. Enteral. Nutr, 4, pp.544~547 (1980)
- Christensen, A. C., "Factors affecting efficacy of methionine hydroxy analogue for chicks fed amino acid diets", Poult. Sci, 59, pp.2480~2484 (1980)
- Chung, T. K., "Utilization of methionine isomers and analogs by the pig", Can. J. Anim. Sci, 72, pp.185~188 (1992)
- Coma, J., "Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs", J. Anim. Sci, 73, pp.472~481 (1995)
- Cromwell, G. L, "Identifying the limiting amino acids in complex and cereal grain-based diets to minimize nitrogen excretion", Midwest Swine Nutr. Conf. Proc, pp.69~83 (2004), The Ohio Univ. Press
- Dibner, J. J., "Capacity in the liver of the broiler chick for conversion of supplemental methionine activity to L-methionine", Poult. Sci, 71, pp.700~708 (1992)
- Dilger, R. N., "DL-Methionine is as efficacious as L-methionine, but modest L-cystine excesses are anorexigenic in sulfur amino acid-deficient purified and practical-type diets fed to chicks", Poult. Sci, 86, pp.2367~2374 (2007)
- Fang, Z., "Methionine metabolism in piglets fed DL-methionine or its hydroxy analogue was affected by distribution of enzymes oxidizing these sources to keto-methionine", J. Agric. Food Chem, 58, pp.2008~2014 (2010)
- Finkelstein, J. D., "Methionine metabolism in mammals", J. Nutr. Biochem, 1, pp.228~237 (1990)
- Gomes, J, "Production of L-methionine by submerged fermentation: A review", Enzyme Microb. Tech, 37, pp.3~18 (2005)
- Garlich, J. D, "Response of broilers to DL-methionine hydroxy analog free acid, DL-methionine, and L-methionine", Poult. Sci, 64, pp.1541~1548 (1985)
- Ikeda, M, "Microbial Production of L-amino Acids", pp.1~35 (2003), Springer
- Katz, R. S., "Efficacy of D-, L- and DLmethionine for growth of chicks fed crystalline amino acid diets", Poult. Sci, 54, pp.1667~1674 (1975)
- Kim, K. I., "Amino acid oxidation by young pigs receiving diets with varying levels of sulphur amino acids", Br. J. Nutr, 50, pp.383~390 (1983)
- Kim, S. Y, "Microorganism producing L-methionine precursor and method of producing Lmethionine and organic acid from the L-methionine precursor. US Patent 9029105", ~ (2015)
- Kumar, D., "Methionine production by fermentation", Biotechnol. Adv, 23, pp.41~61 (2005)
- Mannsfeld, S. P., "Continuous process for the manufacture of methionine. US Patent 04069251", ~ (1978)
- Martín-Venegas, R., "Conversion of the methionine hydroxy analogue DL-2-hydroxy-(4- methylthio) butanoic acid to sulfur-containing amino acids in the chicken small intestine", Poult. Sci, 85, pp.1932~1938 (2006)
- Metayer, S., "Mechanisms through which sulfur amino acids control protein metabolism and oxidative status", J. Nutr. Biochem, 19, pp.207~215 (2008)
- NRC, "Nutrient Requirements of Swine" (1998), National Academic Press
- Odunfa, S. A, "Evaluation of lysine and methionine production in some lactobacilli and yeasts from Ogi", Int. J. Food Microbiol, 63, pp.159~163 (2001)
- Riedijk, M. A., "Methionine transmethylation and transsulfuration in the piglet gastrointestinal tract", Proc. Natl. Acad. Sci. USA, 104, pp.3408~3413 (2007)
- Shen, Y. B, "Effect of feed grade L-methionine on growth performance and gut health in nursery pigs compared with conventional DL-methionine", J. Anim. Sci, 92, pp.5530~5539 (2014)
- Shoveller, A. K., "Dietary cysteine reduces the methionine requirement by an equal proportion in both parenterally and enterally fed piglets", J. Nutr, 133, pp.4215~4224 (2003)
- Shoveller, A. K, "Nutritional and functional importance of intestinal sulfur amino acid metabolism", J. Nutr, 135, pp.1609~1612 (2005)
- Stoll, B, "Catabolism dominates the first-pass intestinal metabolism of dietary essential amino acids in milk protein-fed piglets", J. Nutr, 128, pp.606~614 (1998)
- Tipton, H. C., "A comparison of D-, L-, DL-methionine and methionine hydroxy analogue calcium in chick diets", Poult. Sci, 45, pp.381~387 (1966)
- Zhang, S., "Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs", Amino Acids, 45, pp.1191~1205 (2013)