Influences of somatic donor cell sex on in vitro and in vivo embryo development following somatic cell nuclear transfer in pigs
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts. Methods: Male and female fetal fibroblasts were transferred to in vitro-matured enucleated oocytes and in vitro and in vivo developmental competence of reconstructed embryos was investigated. And, a total of 6,789 female fibroblast nuclear-transferred embryos were surgically transferred into 41 surrogate gilts and 4,746 male fibroblast nuclear-transferred embryos were surgically transferred into 25 surrogate gilts. Results: The competence to develop into blastocysts was not significantly different between the sexes. The mean cell number of female and male cloned blastocysts obtained by in vivo culture (143.8±10.5 to 159.2±14.8) was higher than that of in vitro culture of somatic cell nuclear transfer (SCNT) groups (31.4±8.3 to 33.4±11.1). After embryo transfer, 5 pregnant gilts from each treatment delivered 15 female and 22 male piglets. The average birth weight of the cloned piglets, gestation length, and the postnatal survival rates were not significantly different (p<0.05) between sexes. Conclusion: The present study found that the sex difference of the nuclear donor does not affect the developmental rate of porcine SCNT embryos. Furthermore, postnatal survivability of the cloned piglets was not affected by the sex of the donor cell.
Abstract
Objective: The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts. Methods: Male and female fetal fibroblasts were transferred to in vitro-matured enucleated oocytes and in vitro and in vivo developmental competence of reconstructed embryos was investigated. And, a total of 6,789 female fibroblast nuclear-transferred embryos were surgically transferred into 41 surrogate gilts and 4,746 male fibroblast nuclear-transferred embryos were surgically transferred into 25 surrogate gilts. Results: The competence to develop into blastocysts was not significantly different between the sexes. The mean cell number of female and male cloned blastocysts obtained by in vivo culture (143.8±10.5 to 159.2±14.8) was higher than that of in vitro culture of somatic cell nuclear transfer (SCNT) groups (31.4±8.3 to 33.4±11.1). After embryo transfer, 5 pregnant gilts from each treatment delivered 15 female and 22 male piglets. The average birth weight of the cloned piglets, gestation length, and the postnatal survival rates were not significantly different (p<0.05) between sexes. Conclusion: The present study found that the sex difference of the nuclear donor does not affect the developmental rate of porcine SCNT embryos. Furthermore, postnatal survivability of the cloned piglets was not affected by the sex of the donor cell.
참고문헌 (33)
- Wilmut I, "Viable offspring derived from fetal and adult mammalian cells", Nature, 385, pp.810~813 (1997)
- Betthauser J, "Production of cloned pigs from in vitro systems", Nat Biotechnol, 18, pp.1055~1059 (2000)
- Onishi A, "Pig cloning by microinjection of fetal fibroblast nuclei", Science, 289, pp.1188~1190 (2000)
- Polejaeva IA, "Cloned pigs produced by nuclear transfer from adult somatic cells", Nature, 407, pp.86~90 (2000)
- Prather RS, "Genetically modified pigs for medicine and agriculture", Biotechnol Genet Eng Rev, 25, pp.245~265 (2008)
- Schmidt M, "Pregnancies and piglets from large white sow recipients after two transfer methods of cloned and transgenic embryos of different pig breeds", Theriogenology, 74, pp.1233~1240 (2010)
- Vajta G, "Establishment of an efficient somatic cell nuclear transfer system for production of transgenic pigs", Theriogenology, 77, pp.1263~1274 (2012)
- Boquest AC, "Production of cloned pigs from cultured fetal fibroblast cells", Biol Reprod, 66, pp.1283~1287 (2002)
- Walker SC, "A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes", Cloning Stem Cells, 4, pp.105~112 (2004)
- Koo OJ, "Effect of recipient breed on delivery rate of cloned miniature pig", Zygote, 17, pp.203~207 (2009)
- Petersen B, "Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts", Cloning Stem Cells, 10, pp.355~362 (2008)
- Huang Y, "Efficiency of porcine somatic cell nuclear transfer - a retrospective study of factors related to embryo recipient and embryos transferred", Biol Open, 2, pp.1223~1228 (2013)
- Rim CH, "The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency", Anim Reprod Sci, 143, pp.91~96 (2013)
- Liu Y, "In vitro manipulation techniques of porcine embryos: a meta-analysis related to transfers, pregnancies and piglets", Reprod Fertil Dev, 27, pp.429~439 (2014)
- Li Z, "Effects of donor fibroblast cell type and transferred cloned embryo number on the efficiency of pig cloning", Cell Reprogram, 15, pp.35~42 (2013)
- Shi J, "Influence of embryo handling and transfer method on pig cloning efficiency", Anim Reprod Sci, 154, pp.121~127 (2015)
- Wei H, "Comparison of the efficiency of Banna miniature inbred pig somatic cell nuclear transfer among different donor cells", PLoS ONE, 8, pp.e57728~ (2013)
- Kato Y, "Cloning of calves from various somatic cell types of male and female adult, newborn and fetal cows", J Reprod Fertil, 120, pp.231~237 (2000)
- Wakayama T, "Mouse cloning with nucleus donor cells of different age and type", Mol Reprod Dev, 58, pp.376~383 (2001)
- Kurome M, "Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer", J Reprod Dev, 54, pp.156~163 (2008)
- Yin XJ, "Nuclear remodelling and the developmental potential of nuclear transferred porcine oocytes under delayed-activated conditions", Zygote, 11, pp.167~174 (2003)
- Wakayama T, "Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei", Nature, 394, pp.369~374 (1998)
- Cho JK, "Development of bovine oocytes reconstructed with different donor somatic cells with or without serum starvation", Theriogenology, 57, pp.1819~1828 (2002)
- Pandey A, "Comparative potential of cultured skin fibroblast, cumulus, and granulosa cell to produce somatic cell nuclear transfer (SCNT) preimplantation embryos in buffaloes (Bubalus bubalis) in relation to gene expressions", Cell Reprogram, 12, pp.357~368 (2010)
- Lee GS, "Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations", Theriogenology, 59, pp.1949~1957 (2003)
- Rideout WM III, "Nuclear cloning and epigenetic reprogramming of the genome", Science, 293, pp.1093~1098 (2001)
- Yamazaki Y, "Assessment of the developmental totipotency of neural cells in the cerebral cortex of mouse embryo by nuclear transfer", Proc Natl Acad Sci USA, 98, pp.14022~14026 (2001)
- Huan Y, "Ovulation statuses of surrogate gilts are associated with the efficiency of excellent pig cloning", PLoS ONE, 10, pp.e0142549~ (2015)
- Yin XJ, "Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes", Biol Reprod, 67, pp.442~446 (2002)
- Martin M, "Pre-weaning performance and health of pigs born to cloned (fetal cell derived) swine versus non-cloned swine", Theriogenology, 62, pp.113~122 (2004)
- Hirayama H, "Prepartum maternal plasma glucose concentrations and placental glucose transporter mRNA expression in cows carrying somatic cell clone fetuses", J Reprod Dev, 57, pp.57~61 (2011)
- Hill JR, "Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses", Biol Reprod, 63, pp.1787~1794 (2000)
- Heyman Y, "Frequency and occurrence of late-gestation losses from cattle cloned embryos", Biol Reprod, 66, pp.6~13 (2002)