Effects of different ratios and storage periods of liquid brewer’s yeast mixed with cassava pulp on chemical composition, fermentation quality and in vitro ruminal fermentation
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Objective: This study aims to evaluate the chemical composition, fermentation quality and in vitro ruminal fermentation of various ratios and storage periods of liquid brewer’s yeast (LBY) mixed with cassava pulp (CVP). Methods: Four mixtures of fresh LBY and CVP were made (LBY0, LBY10, LBY20, and LBY30 for LBY:CVP at 0:100, 10:90, 20:80, and 30:70, respectively) on a fresh matter basis, in 500 g in plastic bags and stored at 30 to 32°C. After storage, the bags were opened weekly from weeks 0 to 4. Fermentation quality and in vitro gas production (IVGP) were determined, as well as the dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber, acid detergent fiber and acid detergent lignin contents. Results: The contents of CP and EE increased, whereas all other components decreased, in proportion to LBY inclusion (p<0.01). The DM and OM contents gradually decreased in weeks 3 and 4 (p<0.05), while EE contents were lowest in week 0. The pH, ammonia nitrogen per total nitrogen (NH3-N/TN) and V-score in each mixture and storage period demonstrated superior fermentation quality (pH≤4.2, NH3-N/TN≤12.5%, and V-score>90%). The pH increased and NH3-N/TN decreased, with proportionate increases of LBY, whereas the pH decreased and NH3-N/TN increased, as the storage periods were extended (p<0.01). Although IVGP decreased in proportion to the amount of LBY inclusion (p<0.01), in vitro organic matter digestibility (IVOMD) was unaffected by the mixture ratios. The highest IVGP and IVOMD were observed in week 0 (p<0.01). Conclusion: The inclusion of LBY (as high as 30%) into CVP improves the chemical composition of the mixture, thereby increasing the CP content, while decreasing IVGP, without decreasing fermentation quality and IVOMD. In addition, a preservation period of up to four weeks can guarantee superior fermentation quality in all types of mixtures. Therefore, we recommend limiting the use of CVP as a feed ingredient, given its low nutritional value and improving feed quality with the inclusion of LBY.
Abstract
Objective: This study aims to evaluate the chemical composition, fermentation quality and in vitro ruminal fermentation of various ratios and storage periods of liquid brewer’s yeast (LBY) mixed with cassava pulp (CVP). Methods: Four mixtures of fresh LBY and CVP were made (LBY0, LBY10, LBY20, and LBY30 for LBY:CVP at 0:100, 10:90, 20:80, and 30:70, respectively) on a fresh matter basis, in 500 g in plastic bags and stored at 30 to 32°C. After storage, the bags were opened weekly from weeks 0 to 4. Fermentation quality and in vitro gas production (IVGP) were determined, as well as the dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber, acid detergent fiber and acid detergent lignin contents. Results: The contents of CP and EE increased, whereas all other components decreased, in proportion to LBY inclusion (p<0.01). The DM and OM contents gradually decreased in weeks 3 and 4 (p<0.05), while EE contents were lowest in week 0. The pH, ammonia nitrogen per total nitrogen (NH3-N/TN) and V-score in each mixture and storage period demonstrated superior fermentation quality (pH≤4.2, NH3-N/TN≤12.5%, and V-score>90%). The pH increased and NH3-N/TN decreased, with proportionate increases of LBY, whereas the pH decreased and NH3-N/TN increased, as the storage periods were extended (p<0.01). Although IVGP decreased in proportion to the amount of LBY inclusion (p<0.01), in vitro organic matter digestibility (IVOMD) was unaffected by the mixture ratios. The highest IVGP and IVOMD were observed in week 0 (p<0.01). Conclusion: The inclusion of LBY (as high as 30%) into CVP improves the chemical composition of the mixture, thereby increasing the CP content, while decreasing IVGP, without decreasing fermentation quality and IVOMD. In addition, a preservation period of up to four weeks can guarantee superior fermentation quality in all types of mixtures. Therefore, we recommend limiting the use of CVP as a feed ingredient, given its low nutritional value and improving feed quality with the inclusion of LBY.
참고문헌 (37)
- Aliyu S, "Brewer’s spent grain: A review of its potentials and applications", Afr J Biotechnol, 10, pp.324~331 (2011)
- Levic J, "Use of new feed from brewer by-products for breeding layers", Rom Bio Lett, 15, pp.5559~5565 (2010)
- Manzano M, "Microbiological analyses of dry and slurry yeasts for brewing", J Inst Brew, 111, pp.203~208 (2005)
- Bruning CL, "Characteristics of live and killed brewer’s yeast slurries and intoxication by intraruminal administration to cattle", J Anim Sci, 66, pp.585~591 (1987)
- Linton JH, "Nutritional evaluation of brewery by-products. Review of nutrition papers from Brewers Feed Conference", Feedstuffs, 51, pp.8~ (1977)
- Grieve DG, "Feed intake and growth of cattle fed liquid brewer’s yeast", Can J Anim Sci, 59, pp.89~94 (1979)
- Steckley JD, "Brewer's yeast slurry. II. A source of supplementary protein for lactating dairy cattle", J Dairy Sci, 62, pp.947~953 (1979)
- "Khon Kaen Brewery"[URL]
- "Office of Agricultural Economics"[URL]
- Yimmongkol S, "Research and development projects on improvement of the potential use of dried cassava pulp and cassava leaf meal in concentrate of feedlot cattle", Ph.D., pp.2009
- Jintanawit W, "A study of changing of population of lactic acid bacteria and yeast during ensilaging of cassava pulp", Proceedings of the 44th Kasetsart University Annual Conference, pp.131~137 (2006), Kasetsart University
- Kosugi A, "Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase", Renew Energy, 34, pp.1354~1358 (2009)
- Kosoom W, "Physical, chemical and biological properties of cassava pulp", Proceedings of the 47th Kasetsart University Annual Conference, pp.117~124 (2009), Kasetsart University
- Kaewwongsa W, "Protein enrichment of cassava pulp fermentation by Saccharomyces cerevisiae", J Anim Vet Adv, 10, pp.2434~2440 (2011)
- Chumpawadee S, "Nutrient enrichment of cassava starch industry by-product using rumen microorganism as inoculums source", Pak J Nutr, 8, pp.1380~1382 (2009)
- Thongkratoke R, "Protein enrichment of cassava pulp using microorganisms fermentation techniques for use as an alternative animal feedstuff", J Anim Vet Adv, 9, pp.2859~2862 (2010)
- Vorachinda R, "Improvement of protein content in cassava pulp by fungi fermentation", The 3rd International Conference on Sustainable Animal Agriculture for Developing Countries, pp.307~311 (2011)
- Society of Utilization of Self Supplied Feeds, "The Guidebook for Quality Evaluation of Forage" (2009), Japan Grassland Agriculture and Forage Seed Association
- AOAC, "Official Methods of Analysis" (2000), Association of Official Analytical Chemists
- Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)
- Cai Y, "Field and laboratory methods for grassland science", pp.279~283 (2004), Japan Livestock Technology Association
- Yang XL, "Influence of moisture content on the silage quality of Lolium multiflorum", J Anim Vet Adv, 13, pp.702~705 (2014)
- Menke KH, "Estimation of energetic feed value obtained from chemical analysis and in vitro gas production", Anim Res Dev, 28, pp.7~55 (1988)
- SAS Institute, "User’s Guide: Statistics" (1996), SAS Institute Inc
- Steel RGD, "Principles and Procedure of Statistics: A biometrical approach" (1980), McGraw-Hill Book Company
- Steckley JD, "Brewer's yeast slurry, I. Composition as affected by length of storage, temperature, and chemical treatment", J Dairy Sci, 62, pp.941~946 (1979)
- Adeyemi OA, "Enhancing the nutritional value of whole cassava root meal by rumen filtrate fermentation", Arch Zootec, 56, pp.261~264 (2007)
- Lounglawan P, "Silage production from cassava peel and cassava pulp as energy source in cattle diets", J AnimVet Adv, 10, pp.1007~1011 (2011)
- Umana R, "Effects of a microbial inoculant and (or) sugarcane molasses on the fermentation, aerobic stability, and digestibility of bermudagrass ensiled at two moisture contents", J Anim Sci, 69, pp.4588~4601 (1991)
- Nishino N, "Evaluation of fermentation and aerobic stability of wet brewers grains ensiled alone or in combination with various feeds as a total mixed ration", J Sci Food Agric, 83, pp.557~563 (2003)
- Wang F, "Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability", J Sci Food Agric, 88, pp.133~140 (2008)
- Dinh Van Dung, "Effect of Crude Protein Levels in Concentrate and Concentrate Levels in Diet on In vitro Fermentation", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 27(6), pp.797~805 (2014), 아세아·태평양축산학회[DOI]
- "Metabolizable energy evaluation of pangola grass hay, cassava chip, cassava pulp and brewery waste in Thai native cattle" (2009)[URL]
- Suksombat W, "Energy and protein evaluation of five feedstuffs used in diet in which cassava pulp as main energy source for lactating dairy cow", Suranaree J Sci Technol, 14, pp.99~107 (2006)
- Cerrillo MA, "In vitro gas production parameters in cacti and tree species commonly consumed by grazing goats in a semi arid region of North Mexico", Livest Res Rural Dev, 16, pp.4~9 (2004)
- Getachew G, "Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds", Anim Feed Sci Technol, 111, pp.57~71 (2004)
- Coblentz WK, "Unique interrelationships between fiber composition, water-soluble carbohydrates, and in vitro gas production for fall-grown oat forages", J Dairy Sci, 96, pp.7195~7209 (2013)