Effects of zinc bearing palygorskite supplementation on the growth performance, hepatic mineral content, and antioxidant status of broilers at early age
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Objective: This study was conducted to investigate effects of zinc (Zn) bearing palygorskite (ZnPal) supplementation on growth performance, hepatic mineral content, and antioxidant status of broilers at early age. Methods: A total of 240 1-day-old Arbor Acres broiler chicks were allocated into 5 treatments with 6 replicates of 8 chicks each. Birds in 5 treatments were fed a basal diet supplemented with 0 (Control group; Analyzed Zn content: 81 mg/kg), 20, 40, 60, and 80 mg/kg Zn as ZnPal for 21 days, respectively. Blood, liver and intestinal mucosa were collected at 21 days of age. Results: Treatments did not affect growth performance of broilers during the 21-day study (p> 0.05). The contents of hepatic Zn and magnesium (Mg) were linearly increased (p<0.001) by ZnPal supplementation. ZnPal inclusion linearly (p = 0.007) reduced malondialdehyde (MDA) concentration in serum. The activity of total superoxide dismutase (T-SOD) in liver increased linearly (p = 0.001) with concentration of ZnPal in diet. ZnPal inclusion linearly (p = 0.036) and quadratically (p = 0.005) increased T-SOD activity, and linearly (p = 0.012) increased copper/zinc superoxide dismutase (Cu/Zn SOD) activity in jejunal mucosa. The maximum responses of hepatic and jejunal antioxidant enzymes activities (T-SOD and Cu/Zn SOD) were found when supplementing the basal diet with 60 mg/kg Zn as ZnPal. Furthermore, ZnPal supplementation quadratically (p = 0.001) increased Cu/Zn SOD activity in ileal mucosa, and its maximum activity was observed in the diet supplemented with 20 mg/kg Zn as ZnPal. Conclusion: ZnPal supplementation did not alter growth performance of broilers. Dietary ZnPal inclusion could increase concentrations of hepatic trace minerals (Zn and Mg) and inhibit lipid peroxidation by reducing serum MDA accumulation, with the optimal dosage of Zn from ZnPal being 80 mg/kg diet (analyzed Zn content in the diet: 165 mg/kg), and 60 mg/kg Zn as ZnPal (analyzed Zn content in the diet: 148 mg/kg) was the optimum dosage for broilers to achieve maximum antioxidant enzyme activities.
Abstract
Objective: This study was conducted to investigate effects of zinc (Zn) bearing palygorskite (ZnPal) supplementation on growth performance, hepatic mineral content, and antioxidant status of broilers at early age. Methods: A total of 240 1-day-old Arbor Acres broiler chicks were allocated into 5 treatments with 6 replicates of 8 chicks each. Birds in 5 treatments were fed a basal diet supplemented with 0 (Control group; Analyzed Zn content: 81 mg/kg), 20, 40, 60, and 80 mg/kg Zn as ZnPal for 21 days, respectively. Blood, liver and intestinal mucosa were collected at 21 days of age. Results: Treatments did not affect growth performance of broilers during the 21-day study (p> 0.05). The contents of hepatic Zn and magnesium (Mg) were linearly increased (p<0.001) by ZnPal supplementation. ZnPal inclusion linearly (p = 0.007) reduced malondialdehyde (MDA) concentration in serum. The activity of total superoxide dismutase (T-SOD) in liver increased linearly (p = 0.001) with concentration of ZnPal in diet. ZnPal inclusion linearly (p = 0.036) and quadratically (p = 0.005) increased T-SOD activity, and linearly (p = 0.012) increased copper/zinc superoxide dismutase (Cu/Zn SOD) activity in jejunal mucosa. The maximum responses of hepatic and jejunal antioxidant enzymes activities (T-SOD and Cu/Zn SOD) were found when supplementing the basal diet with 60 mg/kg Zn as ZnPal. Furthermore, ZnPal supplementation quadratically (p = 0.001) increased Cu/Zn SOD activity in ileal mucosa, and its maximum activity was observed in the diet supplemented with 20 mg/kg Zn as ZnPal. Conclusion: ZnPal supplementation did not alter growth performance of broilers. Dietary ZnPal inclusion could increase concentrations of hepatic trace minerals (Zn and Mg) and inhibit lipid peroxidation by reducing serum MDA accumulation, with the optimal dosage of Zn from ZnPal being 80 mg/kg diet (analyzed Zn content in the diet: 165 mg/kg), and 60 mg/kg Zn as ZnPal (analyzed Zn content in the diet: 148 mg/kg) was the optimum dosage for broilers to achieve maximum antioxidant enzyme activities.
참고문헌 (28)
- Rodriguez C, "Regulation of antioxidant enzymes: a significant role for melatonin", J Pineal Res, 36, pp.1~9 (2004)
- Birben E, "Oxidative stress and antioxidant defense", World Allergy Organ J, 5, pp.9~19 (2012)
- Park SY, "Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction", Biol Trace Elem Res, 101, pp.147~163 (2004)
- Salim HM, "Zinc in broiler feeding and nutrition", Avian Biol Res, 1, pp.5~18 (2008)
- Bun SD, "Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella", Poult Sci, 90, pp.1220~1226 (2011)
- Huang YL, "Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet", J Anim Sci, 87, pp.2038~2046 (2009)
- Perry JJP, "The structural biochemistry of the superoxide dismutases", Biochim Biophys Acta, 1804, pp.245~262 (2010)
- Powell SR, "The antioxidant properties of zinc", J Nutr, 130, pp.1447~1454 (2000)
- Zago MP, "The antioxidant properties of zinc: interactions with iron and antioxidants", Free Radic Biol Med, 31, pp.266~274 (2001)
- Zhao CY, "Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers", Biol Trace Elem Res, 160, pp.361~367 (2014)
- Sahin K, "Zinc supplementation alleviates heat stress in laying Japanese quail", J Nutr, 133, pp.2808~2811 (2003)
- Sahin K, "Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail", Poult Sci, 84, pp.882~887 (2005)
- Bergaya F, "Developments in Clay Science vol. 5" (2013), Elsevier
- EU Commission, "Regulation (EC) No. 1831", 2003 of the European Parliament and of the Council of 23 September 2003 on additives for use in animal nutrition, 2003, pp.268, 29~43
- Ministry of Agriculture of China, "Feed raw materials catalogue No. 2038", ~ (2013)
- Yan R, "Bioavailability evaluation of zinc-bearing palygorskite as a zinc source for broiler chickens", Appl Clay Sci, 119, pp.155~160 (2016)
- Yang WL, "An evaluation of zinc bearing palygorskite inclusion on the growth performance, mineral content, meat quality, and antioxidant status of broilers", Poult Sci, 95, pp.878~885 (2016)
- Tang ZG, "Effects of zinc-bearing clinoptilolite on growth performance, cecal microflora and intestinal mucosal function of broiler chickens", Anim Feed Sci Technol, 189, pp.98~106 (2014)
- Tang ZG, "Effect of zinc-bearing zeolite clinoptilolite on growth performance, zinc accumulation, and gene expression of zinc transporters in broilers", J Anim Sci, 93, pp.620~626 (2015)
- Huang YL, "An optimal dietary zinc level of broiler chicks fed a corn-soybean meal diet", Poult Sci, 86, pp.2582~2589 (2007)
- Sunder GS, "Effects of higher levels of zinc supplementation on performance, mineral availability, and immune competence in broiler chickens", J Appl Poult Res, 17, pp.79~86 (2008)
- Liu ZH, "Effects of supplemental zinc source and level on growth performance, carcass traits, and meat quality of broilers", Poult Sci, 90, pp.1782~1790 (2011)
- Li LF, "Zinc–bearing zeolite clinoptilolite improves tissue zinc accumulation in laying hens by enhancing zinc transporter gene mRNA abundance", Anim Sci J, 86, pp.782~789 (2015)
- Hu CH, "Effects of montmorillonite–zinc oxide hybrid on performance, diarrhea, intestinal permeability and morphology of weanling pigs", Anim Feed Sci Technol, 177, pp.108~115 (2012)
- Fridovich I, "Superoxide anion radical (O2-—), superoxide dismutases, and related matters", J Biol Chem, 272, pp.18515~18517 (1997)
- MatÉs JÉM, "Antioxidant enzymes and human diseases", Clin Biochem, 32, pp.595~603 (1999)
- Nielsen F, "Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors", Clin Chem, 43, pp.1209~1214 (1997)
- Tapiero H, "Trace elements in human physiology and pathology: zinc and metallothioneins", Biomed Pharmacother, 57, pp.399~411 (2003)