Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
Abstract
Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
참고문헌 (19)
- Shiranita K, "Grading meat quality by image processing", Pattern Recogn, 33, pp.97~104 (2000)
- Oka A, "Influence of vitamin A on the quality of beef from the Tajima strain of Japanese black cattle", Meat Sci, 48, pp.159~167 (1998)
- Gibb D, "Removal of supplemental vitamin A from barley-based diets improves marbling in feedlot heifers", Canadian J Anim Sci, 91, pp.669~674 (2011)
- Ward AK, "The impact of vitamin A restriction and ADH1C genotype on marbling in feedlot steers", J Anim Sci, 90, pp.2476~2483 (2012)
- Molotkov A, "Excessive vitamin A toxicity in mice genetically deficient in either alcohol dehydrogenase Adh1 or Adh3", Eur J Biochem, 269, pp.2607~2612 (2002)
- Molotkov A, "Genetic evidence that retinaldehyde dehydrogenase Raldh1 (Aldh1a1) functions downstream of alcohol dehydrogenase Adh1 in metabolism of retinol to retinoic acid", J Biol Chem, 278, pp.36085~36090 (2003)
- S.S.Moon, "Carcass Traits Determinin Quality and Yield Grades of Hanwoo Streers", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 16(7), pp.1049~1054 (2003), 아세아·태평양축산학회
- Pickworth CL, "Restriction of vitamin A and D in beef cattle finishing diets on feedlot performance and adipose accretion", J Anim Sci, 90, pp.1866~1878 (2012)
- Gorocica-Buenfil MA, "Effect of vitamin A restriction on carcass characteristics and immune status of beef steers", J Anim Sci, 86, pp.1609~1616 (2008)
- Adachi K, "Relationship between serum biochemical values and marbling scores in Japanese Black steers", J Vet Med Sci, 61, pp.961~964 (1999)
- National Research Council, "Subcommittee on beef cattle nutrition. Nutrient requirements of beef cattle" (1996), National Academy Press
- Krone KG, "Interaction of vitamin A supplementation level with ADH1C genotype on intramuscular fat in beef steers", Animal, 10, pp.403~409 (2016)
- Kiefer FW, "Retinaldehyde dehydrogenase 1 coordinates hepatic gluconeogenesis and lipid metabolism", Endocrinology, 153, pp.3089~3099 (2012)
- Napoli JL, "Enzymes and binding proteins affecting retinoic acid concentrations", J Steroid Biochem Mol Biol, 53, pp.497~502 (1995)
- Kim DM, "Retinoic acid inhibits adipogenesis via activation of Wnt signaling pathway in 3T3-L1 preadipocytes", Biochem Biophys Res Commun, 434, pp.455~459 (2013)
- Sagara C, "Molecular mechanism of 9-cis-retinoic acid inhibition of adipogenesis in 3T3-L1 cells", Biochem Biophys Res Commun, 433, pp.102~107 (2013)
- Berry DC, "All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta", delta and retinoic acid receptor, 2009, pp.29, 3286~3296
- Mizoguchi Y, "Effect of retinoic acid on gene expression profiles of bovine intramuscular preadipocytes during adipogenesis", Anim Sci J, 85, pp.101~111 (2014)
- Amengual J, "Retinoic acid treatment enhances lipid oxidation and inhibits lipid biosynthesis capacities in the liver of mice", Cell Physiol Biochem, 25, pp.657~666 (2010)