본문 바로가기
KERD
논문KCI 등재

Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs. Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70, and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed. Results: The results showed that supplemental dietary CC increased (p<0.05) the muscle fiber density. And CC supplementation also up-regulated (p<0.05) the expression of myosin heavy chain 1 (MyHC1) and MyHC2x mRNA levels, and down-regulated (p<0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (p<0.05) the concentration of total cholesterol in the plasma and enhanced (p<0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of chloramphenicol acetyltransferase 2, b0,+ amino acid transporter, and y+-L-type amino acid transporter 1 were up-regulated (p<0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg. Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

Abstract

Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs. Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70, and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed. Results: The results showed that supplemental dietary CC increased (p<0.05) the muscle fiber density. And CC supplementation also up-regulated (p<0.05) the expression of myosin heavy chain 1 (MyHC1) and MyHC2x mRNA levels, and down-regulated (p<0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (p<0.05) the concentration of total cholesterol in the plasma and enhanced (p<0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of chloramphenicol acetyltransferase 2, b0,+ amino acid transporter, and y+-L-type amino acid transporter 1 were up-regulated (p<0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg. Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

참고문헌 (38)

  1. Grunert KG, "Consumer perception of meat quality and implications for product development in the meat sector—a review", Meat Sci, 66, pp.259~272 (2004)
  2. Lefaucheur L, "Myosin heavy chain composition of different skeletal muscles in large white and meishan pigs", J Anim Sci, 82, pp.1931~1941 (2004)
  3. Joo ST, "Control of fresh meat quality through manipulation of muscle fiber characteristics", Meat Sci, 95, pp.828~836 (2013)
  4. Lee S, "Skeletal muscle fiber type and myofibrillar proteins in relation to meat quality", Meat Sci, 86, pp.166~170 (2010)
  5. Kim GD, "Intramuscular variations of proteome and muscle fiber type distribution in semimembranosus and semitendinosus muscles associated with pork quality", Food Chem, 244, pp.143~152 (2018)
  6. Zhou P, "Effects of dietary crude protein levels and cysteamine supplementation on protein synthetic and degradative signaling in skeletal muscle of finishing pigs", Plos One, 10, pp.e0139393~ (2015)
  7. Zhang ZY, "Cysteamine alleviates early brain injury via reducing oxidative stress and apoptosis in a rat experimental subarachnoid hemorrhage model", Cell Mol Neurobiol, 35, pp.543~553 (2015)
  8. Bai MM, "Effects of dietary coated cysteamine hydrochloride on pork color in finishing pigs", J Sci Food Agric, 48, pp.96~100 (2017)
  9. Liu G, "Effects of dietary cysteamine supplementation on growth performance and whole-body protein turnover in finishing pigs", Livest Sci, 122, pp.86~89 (2009)
  10. Zhou P, "Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs", Anim Sci J, 88, pp.314~321 (2017)
  11. NRC, "Nutrient requirements of swine" (2012), National Academy Press
  12. Deng J, "Dietary amylose and amylopectin ratio and resistant starch content affects plasma glucose, lactic acid, hormone levels and protein synthesis in splanchnic tissues", J Anim Physiol Anim N, 94, pp.220~226 (2010)
  13. Turek Z, "Cardiac hypertrophy, capillary and muscle fiber density, muscle fiber diameter, capillary radius and diffusion distance in the myocardium of growing rats adapted to a simulated altitude of 3500 m", Pflügers Archiv, 335, pp.19~28 (1972)
  14. Qin Ping Yu, "Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes", Animal Bioscience, 30(11), pp.1620~1632 (2017), 아세아·태평양축산학회[DOI]
  15. Hu CJ, "Dietary supplementation with arginine and glutamic acid modifies growth performance, carcass traits, and meat quality in growing-finishing pigs", J Anim Sci, 95, pp.2680~2689 (2017)
  16. Tan B, "Dietary l-arginine supplementation differentially regulates expression of lipid-metabolic genes in porcine adipose tissue and skeletal muscle", J Nutr Biochem, 22, pp.441~445 (2011)
  17. Zhang C, "Dietary resveratrol supplementation improves meat quality of finishing pigs through changing muscle fiber characteristics and antioxidative status", Meat Sci, 102(Supplement C), pp.15~21 (2015)
  18. Yang CB, "Effects of dietary supplementation of cysteamine on growth performance, carcass quality, serum hormones and gastric ulcer in finishing pigs", J Sci Food Agric, 85, pp.1947~1952 (2005)
  19. Barnett M, "Cysteamine : a human health dietary additive with potential to improve livestock growth rate and efficiency", Anim Prod Sci, 56, pp.1330~1338 (2016)
  20. Bai MM, "Effects of dietary coated cysteamine hydrochloride on pork color in finishing pigs", J Sci Food Agric, 98, pp.1743~1750 (2018)
  21. Yu Q, "Unraveling proteome changes of holstein beef m. Semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry", J Proteomics, 154, pp.85~93 (2017)
  22. Ryu YC, "Effects of muscle mass and fiber type composition of longissimus dorsi muscle on postmortem metabolic rate and meat quality in pigs", J Muscle Foods, 17, pp.343~353 (2006)
  23. Jeong DW, "Correlations of trained panel sensory values of cooked pork with fatty acid composition, muscle fiber type, and pork quality characteristics in Berkshire pigs", Meat Sci, 86, pp.607~615 (2010)
  24. Gondret F, "Low birth weight is associated with enlarged muscle fiber area and impaired meat tenderness of the longissimus muscle in pigs", J Anim Sci, 84, pp.93~103 (2006)
  25. Schiaffino S, "Molecular diversity of myofibrillar proteins : gene regulation and functional significance", Physiol Rev, 76, pp.371~423 (1996)
  26. 김갑돈, "The Relationship between Meat Color (CIE L * and a * ), Myoglobin Content, and Their Influence on Muscle Fiber Characteristics and Pork Quality", 한국축산식품학회지, 30(4), pp.626~633 (2010), 한국축산식품학회
  27. Cleveland BD, "Effect of feeding distiller's grains and supplementing with dietary antioxidants on ground beef color during retail display", Meat Sci, 101, pp.110~ (2015)
  28. Korn KT, "Supplemental vitamin D3 and zilpaterol hydrochloride. II. Effect on calcium concentration, muscle fiber type, and calpain gene expression of feedlot steers", J Anim Sci, 91, pp.3332~3340 (2013)
  29. Hu R, "Effects of ghrp-2 and cysteamine administration on growth performance, somatotropic axis hormone and muscle protein deposition in yaks (Bos grunniens) with growth retardation", Plos One, 11, pp.e0149461~ (2016)
  30. Kamalakar RB, "Effect of the degree and duration of early dietary amino acid restrictions on subsequent and overall pig performance and physical and sensory characteristics of pork", J Anim Sci, 87, pp.3596~3606 (2009)
  31. Liu G, "Effects of dietary supplementation with cysteamine on growth hormone receptor and insulin-like growth factor system in finishing pigs", J Agric Food Chem, 56, pp.5422~5427 (2008)
  32. Zhang S, "Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs", Amino Acids, 45, pp.1191~1205 (2013)
  33. Laspiur JP, "Dietary protein intake and stage of lactation differentially modulate amino acid transporter mrna abundance in porcine mammary tissue", J Nutr, 139, pp.1677~1684 (2009)
  34. Wu G, "Impacts of arginine nutrition on embryonic and fetal development in mammals", Amino Acids, 45, pp.241~256 (2013)
  35. He L, "Effects of dietary L-lysine intake on the intestinal mucosa and expression of cat genes in weaned piglets", Amino Acids, 45, pp.383~391 (2013)
  36. Fuchs BC, "Amino acid transporters asct2 and lat1 in cancer: partners in crime?", Semin Cancer Biol, 15, pp.254~266 (2005)
  37. Verrey F, "CATs and HATs : the SLC7 family of amino acid transporters", Pflügers Arch, 447, pp.532~542 (2004)
  38. Mcleod KR, "Cysteamine-induced depletion of somatostatin in sheep : time course of depletion and changes in plasma metabolites, insulin, and growth hormone", J Anim Sci, 73, pp.77~87 (1995)

관련 논문AI추천

Effects of varying levels of coated cysteamine hydrochloride in diet on growth performance and carcass quality of steersHelali MD. Maksudul Haque, Khejornsart Pichad, Mamun Abdullah Al, Meenongyai Watcharawit, Phongkaew Piyamas, Shimul Alimul Islam, Traithilen Unchan, Waramit Naroon, Wongpanit Kannika아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제10호Effects of lysine concentration of the diet on growth performance and meat quality in finishing pigs with high slaughter weights손유민, 장재철, Chul Young Lee, Doo-Hwan Kim, Eun-Yeong Lee, Sang-Hyon Oh, Seon-Tea Joo, Tae-Whan Park, Yeunhae Jung한국축산학회 · 한국축산학회지2023 · 제65권 제6호Inclusion of dietary nontoxic sulfur on growth performance, immune response, sulfur amino acid content and meat characteristics in growing-finishing pigs김유용, Gim Min Jin, jinxinghao, Shin Hae Won아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제5호L-lysine improves pork quality during postmortem aging: insights into meat quality, protein properties, and enzyme activitiesFu Chao, Guo Xiuyun, Meng Xiangren, Xu Shuangyi아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제12호Dietary supplementation of Eucommia leaf extract to growing-finishing pigs alters muscle metabolism and improves meat qualityDeng Chuangye, Guo Qiuping, Li Fengna, Liu Chuxin, Shen Qingwu W., Shen Zhenglei아세아·태평양축산학회 · Animal Bioscience2024 · 제37권 제4호Relationships of Muscle Fiber Characteristics to Dietary Energy Density, Slaughter Weight, and Muscle Quality Traits in Finishing Pigs김갑돈, 박만종, 박병철, 이철영, 정진연, 주선태, 하덕민한국축산학회 · 한국축산학회지2012 · 제54권 제3호

이 간행물의 다른 논문 (8건)