Comparison of in vitro digestibility and chemical composition among four crop straws treated by Pleurotus ostreatus
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Objective: The effects of Pleurotus ostreatus on the feed utilization of broad bean stalks (BBS), rape straw (RS), paddy straw (PS), and corn stalk (CS) was examined. Methods: The four roughages were co-cultured with Pleurotus ostreatus. The chemical composition; enzyme activities of laccase, carboxymethylcellulase (CMCase) and xylanase; carbohydrate and protein fractions (based on The Cornell Net Carbohydrate and Protein System [CNCPS]) were assessed at different days after inoculation (7, 14, 21, 28 d) and un-inoculated roughages (control, 0 d). The digestibility of nutrient components and the gas production of roughage with various incubation times were monitored at 0, 2, 4, 6, 9, 12, 24, 36, 48, 60, and 72 h using an in vitro ruminal fermentation method. Results: A higher CMCase activity (0.1039 U/mL) and earlier time to peak (14 d) were detected in Pleurotus ostreatus cultured with CS (p<0.05). Significantly, the incubation length-dependent responses of cumulative gas production were observed from 24 to 72 hours post fermentation (p<0.05), and these incubation length-dependent effects on cumulative gas production of PS and CS appeared earlier (24 h) for PS and CS than those (48 h) for BBS and RS (p<0.05). The fast-degradable carbohydrate (CA) content for all four roughages significantly increased over time (p<0.05). Nonetheless, increased degradation efficiency for CA treated with Pleurotus ostreatus was detected at both 21 and 28 days of incubation (p<0.05). With the exception of PS (p<0.05), there were no significant difference among the roughages (p>0.05) in slowly-degradable carbohydrate (CB2) at different incubation times (p<0.05). Conclusion: Assessment of the alterations in chemical composition, CNCPS system fractions, and the fermentation kinetics after biological pretreatment may yield a valuable database for evaluating the biological pretreatment of Pleurotus ostreatus in ruminant feed.
Abstract
Objective: The effects of Pleurotus ostreatus on the feed utilization of broad bean stalks (BBS), rape straw (RS), paddy straw (PS), and corn stalk (CS) was examined. Methods: The four roughages were co-cultured with Pleurotus ostreatus. The chemical composition; enzyme activities of laccase, carboxymethylcellulase (CMCase) and xylanase; carbohydrate and protein fractions (based on The Cornell Net Carbohydrate and Protein System [CNCPS]) were assessed at different days after inoculation (7, 14, 21, 28 d) and un-inoculated roughages (control, 0 d). The digestibility of nutrient components and the gas production of roughage with various incubation times were monitored at 0, 2, 4, 6, 9, 12, 24, 36, 48, 60, and 72 h using an in vitro ruminal fermentation method. Results: A higher CMCase activity (0.1039 U/mL) and earlier time to peak (14 d) were detected in Pleurotus ostreatus cultured with CS (p<0.05). Significantly, the incubation length-dependent responses of cumulative gas production were observed from 24 to 72 hours post fermentation (p<0.05), and these incubation length-dependent effects on cumulative gas production of PS and CS appeared earlier (24 h) for PS and CS than those (48 h) for BBS and RS (p<0.05). The fast-degradable carbohydrate (CA) content for all four roughages significantly increased over time (p<0.05). Nonetheless, increased degradation efficiency for CA treated with Pleurotus ostreatus was detected at both 21 and 28 days of incubation (p<0.05). With the exception of PS (p<0.05), there were no significant difference among the roughages (p>0.05) in slowly-degradable carbohydrate (CB2) at different incubation times (p<0.05). Conclusion: Assessment of the alterations in chemical composition, CNCPS system fractions, and the fermentation kinetics after biological pretreatment may yield a valuable database for evaluating the biological pretreatment of Pleurotus ostreatus in ruminant feed.
참고문헌 (32)
- Jakyeom S, "Evaluation of nutritional and economic feed values of spent coffee grounds and Artemisia princeps residues as a ruminant feed using in vitro ruminal fermentation", Peer J, 3, pp.e1343~ (2015)
- Aregheore EM, "Studies on grazing behavior of goats in the Cook Islands: The animal-plant complex in forage preference", palatability phenomena, 2006, pp.8, 147~153
- 김영일, "균상재배 느타리버섯부산물 생균발효사료의 반추동물 조사료원으로서의 가치 평가", 한국축산학회지, 52(2), pp.117~124 (2010), 한국축산학회
- Panagiotou G, "Penicillium brasilianum as an enzyme factory; the essential role of feruloyl esterases for the hydrolysis of the plant cell wall", J Biotechnol, 130, pp.219~228 (2007)
- Jaewon L, "Enzymatic saccharification of biologically pretreated Pinus densiflora using enzymes from brown rot fungi", J Biosci Bioeng, 106, pp.162~167 (2008)
- Kumar R, "Bioconversion of lignocellulosic biomass : biochemical and molecular perspectives", J Ind Microbiol, 35, pp.377~391 (2008)
- Isroi, Millati R, "Biological pretreatment of lignocelluloses with white-rot fungi and its applications: a review", Bioresources, 6, pp.5224~5259 (2011)
- Cohen R, "Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus", Appl Microbiol Biotechnol, 58, pp.582~594 (2002)
- Russell JB, "A net carbohydrate and protein system for evaluating cattle diets : I. Ruminal Fermentation", J Anim Sci, 70, pp.3551~3561 (1992)
- Rahman MM, "Improving ruminal degradability of oil palm fronds using white rot fungi", Anim Feed Sci Technol, 169, pp.157~166 (2011)
- NRC, "Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids" (2007), National Academies Press
- Palmieri G, "A novel white laccase from Pleurotus ostreatus", J Biol Chem, 272, pp.31301~31307 (1997)
- Meddebmouelhi F, "A comparison of plate assay methods for detecting extracellular cellulase and xylanase activity", Enzyme Microb Technol, 66, pp.16~19 (2014)
- Tilley JMA, "A two-stage technique for the in vitro digestion of forage crops", Grass Forage Sci, 18, pp.104~111 (1963)
- Bosch G, "Comparative in vitro fermentation activity in the canine distal gastrointestinal tract and fermentation kinetics of fiber sources", J Anim Sci, 86, pp.2979~2989 (2008)
- Schofield P, "Kinetics of fiber digestion from in vitro gas production", J Anim Sci, 72, pp.2980~2991 (1994)
- AOAC, "Association of Official Analytical Chemists" (1990), AOAC international
- Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)
- Krishnamoorthy U, "Evaluation of a mathematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen-undegraded nitrogen content of feedstuffs", Br J Nutr, 50, pp.555~568 (1983)
- Koukiekolo R, "Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA", Appl Environ Microbiol, 71, pp.3504~3511 (2005)
- Philippoussis A, "Biomass, laccase and endoglucanase production by Lentinula edodes during solid state fermentation of reed grass, bean stalks and wheat straw residues", World J Microbiol Biotechnol, 27, pp.285~297 (2011)
- Kirk TK, "Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium", Arch Microbiol, 117, pp.277~285 (1978)
- Mikiashvili N, "Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity", World J Microbiol Biotechnol, 22, pp.999~1002 (2006)
- Yang X, "Effect of biopretreatment on thermogravimetric and chemical characteristics of corn stover by different white-rot fungi", Bioresour Technol, 101, pp.5475~5479 (2010)
- Yu HB, "The effect of biological pretreatment with the selective white-rot fungus Echinodontium taxodii on enzymatic hydrolysis of softwoods and hardwoods", Bioresour Technol, 100, pp.5170~5175 (2009)
- 서성원, "Degradation Kinetics of Carbohydrate Fractions of Ruminant Feeds Using Automated Gas Production Technique", Animal Bioscience, 22(3), pp.356~364 (2009), 아세아·태평양축산학회
- Akin DE, "Degradation of polysaccharides and lignin by ruminal bacteria and fungi", Appl Environ Microbiol, 54, pp.1117~1125 (1988)
- Fox DG, "The Cornell Net Carbohydrate and Protein System model for evaluating herd nutrition and nutrient excretion", Anim Feed Sci Technol, 112, pp.29~78 (2004)
- Ghoorchi T, "Study of protein characteristic of five feeds by CNCPS model", Asian J Anim Vet Adv, 5, pp.584~591 (2010)
- Krishnamoorthy U, "Nitrogen fractions in selected feedstuffs", J Dairy Sci, 65, pp.217~225 (1982)
- Hall MB, "Differences in yields of microbial crude protein from in vitro fermentation of carbohydrates 1", J Dairy Sci, 84, pp.2486~2493 (2001)
- Ten HR, "Oxidative mechanisms involved in lignin degradation by white-rot fungi", Chem Rev, 101, pp.3397~3414 (2001)