Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objective: The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods: A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results: The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion: In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus.
Abstract
Objective: The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods: A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results: The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion: In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus.
참고문헌 (30)
- Jin H, "Grape seed procyanidin extract attenuates hypoxic pulmonary hypertension by inhibiting oxidative stress and pulmonary arterial smooth muscle cells proliferation", J Nutr Biochem, 36, pp.81~88 (2016)[DOI]
- Li X, "Antimicrobial activity and mechanism of Larch bark procyanidins against Staphylococcus aureus", Acta Biochim Biophys Sin(Shanghai), 49, pp.1058~1066 (2017)[DOI]
- Gessner DK, "Analysis of hepatic transcript profile and plasma lipid profile in early lactating dairy cows fed grape seed and grape marc meal extract", BMC Genomics, 18, pp.253~ (2017)[DOI]
- Correddu F, "Grape seed and linseed, alone and in combination, enhance unsaturated fatty acids in the milk of Sarda dairy sheep", J Dairy Sci, 99, pp.1725~1735 (2016)[DOI]
- Johnson KA, "Methane emissions from cattle", J Anim Sci, 73, pp.2483~2492 (1995)[DOI]
- Ungerfeld EM, "Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation : a meta-analysis", Front Microbiol, 6, pp.37~ (2015)[DOI]
- Ross EM, "Investigating the effect of two methane-mitigating diets on the rumen microbiome using massively parallel sequencing", J Dairy Sci, 96, pp.6030~6046 (2013)[DOI]
- Menke KH, "Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid", Anim Res Dev, 28, pp.7~55 (1988)
- Wang K, "Shifts of hydrogen metabolism from methanogenesis to propionate production in response to replacement of forage fiber with non-forage fiber sources in diets in vitro", Front Microbiol, 9, pp.2764~ (2018)[DOI]
- S.Y. Mao, "Effect of disodium fumarate on ruminal metabolism and rumen bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA", Animal Feed Science and Technology, 140(3-4), pp.293~306 (2008), Elsevier BV[DOI]
- Jin D, "Insights into abundant rumen ureolytic bacterial community using rumen simulation system", Front Microbiol, 7, pp.1006~ (2016)[DOI]
- Vargas JE, "Effect of sunflower and marine oils on ruminal microbiota, in vitro fermentation and digesta fatty acid profile", Front Microbiol, 8, pp.1124~ (2017)[DOI]
- Guo YQ, "Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms", Lett Appl Microbiol, 47, pp.421~426 (2008)[DOI]
- Yoo G, "Effects of biochar addition on greenhouse gas emissions and microbial responses in a short-term laboratory experiment", J Environ Qual, 41, pp.1193~1202 (2012)[DOI]
- Tong J, "Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows", PLoS One, 13, pp.e0198225~ (2018)[DOI]
- Edgar RC, "UCHIME improves sensitivity and speed of chimera detection", Bioinformatics, 27, pp.2194~2200 (2011)[DOI]
- Mokni M, "Effect of grape seed and skin supplement on milk yield and composition of dairy ewes", Trop Anim Health Prod, 49, pp.131~137 (2017)[DOI]
- Leahy SC, "Genome sequencing of rumen bacteria and archaea and its application to methane mitigation strategies", Animal, 7(Suppl 2), pp.235~243 (2013)[DOI]
- Balch WE, "Methanogens : reevaluation of a unique biological group", Microbiol Rev, 43, pp.260~296 (1979)
- Alejandro B, "Study of methanogen communities associated with different rumen protozoal populations", FEMS Microbiol Ecol, 90, pp.663~677 (2014)[DOI]
- Moissl-Eichinger C, "Archaea are interactive components of complex microbiomes", Trends Microbiol, 26, pp.70~85 (2018)[DOI]
- Danielsson R, "Methane production in dairy cows correlates with rumen methanogenic and bacterial community structure", Front Microbiol, 8, pp.226~ (2017)[DOI]
- Moss AR, "Methane production by ruminants : its contribution to global warming", Ann Zootech, 49, pp.231~253 (2000)[DOI]
- Janssen PH, "Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics", Anim Feed Sci Technol, 160, pp.1~22 (2010)[DOI]
- Veneman JB, "Does dietary mitigation of enteric methane production affect rumen function and animal productivity in dairy cows", PLoS ONE, 10, pp.e0140282~ (2015)[DOI]
- Morgavi DP, "Microbial ecosystem and methanogenesis in ruminants", Animal, 4, pp.1024~1036 (2010)[DOI]
- Jolazadeh AR, "Effects of soybean meal treated with tannins extracted from pistachio hulls on performance, ruminal fermentation, blood metabolites and nutrient digestion of Holstein bulls", Anim Feed Sci Technol, 203, pp.33~40 (2015)[DOI]
- Roehe R, "Bovine host genetic variation influences rumen microbial methane production with best selection criterion for low methane emitting and efficiently feed converting hosts based on metagenomic gene abundance", PLoS Genet, 12, pp.e1005846~ (2016)[DOI]
- Patra AK, "Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition", J Sci Food Agric, 91, pp.24~37 (2011)[DOI]
- Council N, "Nutrient requirements of dairy cattle" (2001), National Research Council
