Henoch-Sch？nlein Purpura에서 Interleukin 1β 유전자 다형성과 신장 침범과의 연관성
The Relationship Between Interleukin 1β Gene Polymorphism and Renal Involvement in Henoch-Sch？nlein Purpura
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목적:HSP는 전신성 혈관염을 일으키는 질환으로 피부소견, 관절 증상, 복부증상을 특징으로 하며, 신장침범은 예후와 관계된 중요한 인자로 알려져 있다. Interleukin 1(IL-1)는 항염증반응을 가지고 있는 시토카인으로써 그 유전자는 2번 염색체장완의 q13-q21부위에 위치하며 Interleukin -1 receptor antagonist(IL-ra) 유전자와 인접해 있다. IL-1β 유전자의 높은 mRNA발현이 HSP 환자들의 피부소견에 있어서 보고되었다. 이에 저자들은 HSP로 진단받은 환자들에게서 IL-1β 유전자의 다형성과 신장 침범과의 연관성을 조사하였다.방법:2003년 6월부터 2005년 6 월까지 연세대학교 원주기독병원 소아과를 방문하여 HSP로 진단받은 34명의 환자와 정상 대조군 27명을 대상으로 하였다. EDTA 처리된 전혈에서 DNA를 추출하였으며, IL-1β 유전자의 다형성은 PCR- RFLP로 측정하였다.결과:HSP 환자군과 정상 대조군에서의 IL1β-511위치에서의 유전자 다형성의 T 대립유전자 빈도는 각각 47%, 48%로 유의한 차이는 없었다. HSP군 중 신장 침범이 확인된 경우는 총 16명으로 신장 침범군과 비침범군에서의 T 대립유전자 빈도는 각각 47%, 56%로 양군간에 차이는 없었다.
Abstract
Purpose:High interleukin-1 beta(IL-1β) expression in the skin biopsy specimens of patients with Henoch-Schönlein Purpura(HSP) has been observed. We examined IL-1β gene polymorphism in patients with HSP. The purpose of this study is to examine the relationship between IL-1β gene polymorphism and renal involvement in HSP. Methods:Patients from mideast Korea with HSP were studied. All patients had at least 6 months of follow up. Patients and ethnically matched controls were genotyped for IL-1β gene polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results:Thirty-four patients(all younger than 15 years old) who had been diagnosed with HSP and 27 controls were examined. No allele or genotype differences between the HSP and control groups were observed. No significant association between the carriage of IL-1β(-511) T allele and renal involvement(P=0.525, OR:1.417, CI:0.545-3.686) was found. Conclusion:In unselected patients with HSP, carriage of IL-1β(-511) T allele does not appear to influence renal involvement
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