Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora
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Objective: A series of experiment were conducted to evaluate the effects of replacing a part of soybean meal (SBM) at 6% of broiler diets with fermented soybean meal (FSBM) obtained by single or two-stage fermentation by measuring growth performance, antioxidant activity in the jejunum and distal intestinal microflora. Methods: Soybean meal samples were prepared by single-stage fermentation using Bacillus velezensis (Bv) (FSBMB), or Lactobacillus spp. (as commercial control) (FSBML). Additional SBM sample was prepared by two-stage fermentation using Bv and subsequently using Lactobacillus brevis ATCC 367 (Lb) (FSBMB+L). Enzyme activity, chemical composition, trichloroethanoic acid-nitrogen solubility index (TCA-NSI) and antioxidant activity were measured. Then, in an in vivo study, 320 Ross308 broilers were divided into four groups with ad libitum supply of feed and water. Four groups were fed either a corn-soybean meal diet (SBM), or one of fermented SBM diets (FSBMB+L, FSBMB, and FSBML). Growth, serum characteristics, microflora, and the mRNA expression of selected genes were measured. Results: Compared to SBM, FSBMB+L contained lower galacto-oligosaccharide, allergic protein, and trypsin inhibitor, and higher TCA-NSI by about three times (p<0.05). Reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability correlated positively with the TCA-NSI content in FSBM. Growth performances were not significantly different among four groups. In jejunum of 35-day-old broilers, partial replacement of SBM by FSBMB+L increased the activity of superoxide dismutase and catalase (CAT), and the FSBMB group had the highest catalase activity (p<0.05). Partial replacement of SBM by FSBM increased relative mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and peptide transporter 1 (PepT1) (p<0.05); however, FSBMB+L increased CAT mRNA level to 5 times of the control (p<0.05). Conclusion: Using Bv- and Lb-processed SBM through two-stage fermentation to partially replace 6% of diets will improve the gut's antioxidant activity under commercial breeding in broilers.
Abstract
Objective: A series of experiment were conducted to evaluate the effects of replacing a part of soybean meal (SBM) at 6% of broiler diets with fermented soybean meal (FSBM) obtained by single or two-stage fermentation by measuring growth performance, antioxidant activity in the jejunum and distal intestinal microflora. Methods: Soybean meal samples were prepared by single-stage fermentation using Bacillus velezensis (Bv) (FSBMB), or Lactobacillus spp. (as commercial control) (FSBML). Additional SBM sample was prepared by two-stage fermentation using Bv and subsequently using Lactobacillus brevis ATCC 367 (Lb) (FSBMB+L). Enzyme activity, chemical composition, trichloroethanoic acid-nitrogen solubility index (TCA-NSI) and antioxidant activity were measured. Then, in an in vivo study, 320 Ross308 broilers were divided into four groups with ad libitum supply of feed and water. Four groups were fed either a corn-soybean meal diet (SBM), or one of fermented SBM diets (FSBMB+L, FSBMB, and FSBML). Growth, serum characteristics, microflora, and the mRNA expression of selected genes were measured. Results: Compared to SBM, FSBMB+L contained lower galacto-oligosaccharide, allergic protein, and trypsin inhibitor, and higher TCA-NSI by about three times (p<0.05). Reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability correlated positively with the TCA-NSI content in FSBM. Growth performances were not significantly different among four groups. In jejunum of 35-day-old broilers, partial replacement of SBM by FSBMB+L increased the activity of superoxide dismutase and catalase (CAT), and the FSBMB group had the highest catalase activity (p<0.05). Partial replacement of SBM by FSBM increased relative mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and peptide transporter 1 (PepT1) (p<0.05); however, FSBMB+L increased CAT mRNA level to 5 times of the control (p<0.05). Conclusion: Using Bv- and Lb-processed SBM through two-stage fermentation to partially replace 6% of diets will improve the gut's antioxidant activity under commercial breeding in broilers.
참고문헌 (30)
- He L, "Soybean antigen proteins and their intestinal sensitization activities", Curr Protein Pept Sci, 16, pp.613~621 (2015)[DOI]
- Sanjukta S, "Production of bioactive peptides during soybean fermentation and their potential health benefits", Trends Food Sci Technol, 50, pp.1~10 (2016)[DOI]
- Ashaolu TJ, "Health applications of soy protein hydrolysates", Int J Pept Res Ther, 26, pp.2333~2343 (2020)[DOI]
- Cui J, "A novel fermented soybean, inoculated with selected Bacillus, Lactobacillus and Hansenula strains, showed strong antioxidant and anti-fatigue potential activity", Food Chem, 333, pp.127527~ (2020)[DOI]
- Yang J, "A value-added approach to improve the nutritional quality of soybean meal by-product : Enhancing its antioxidant activity through fermentation by Bacillus amyloliquefaciens SWJS22", Food Chem, 272, pp.396~403 (2019)[DOI]
- Chachaj R, "The effect of partial replacement of soybean meal with fermented soybean meal on chicken performance and immune status", J Anim Feed Sci, 28, pp.263~271 (2019)[DOI]
- Wu P, "Effect of partial replacement of soybean meal with high-temperature fermented soybean meal in antibiotic-growth-promoter-free diets on growth performance, organ weights, serum indexes, intestinal flora and histomorphology of broiler chickens", Anim Feed Sci Technol, 269, pp.114616~ (2020)[DOI]
- Toe CJ, "Extracellular proteolytic activity and amino acid production by lactic acid bacteria isolated from Malaysian foods", Int J Mol Sci, 20, pp.1777~ (2019)[DOI]
- Hati S, "α-Galactosidase activity and oligosaccharides utilization by lactobacilli during fermentation of soy milk", J Food Process Preserv, 38, pp.1065~1071 (2014)[DOI]
- Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Anal Chem, 31, pp.426~428 (1959)[DOI]
- Latimer GW, "Official methods of analysis of AOAC International" (2012), AOAC International
- Xie J, "Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from Mung bean (Vigna radiate)", Food Chem, 270, pp.243~250 (2019)[DOI]
- Yin L, "Proteolysis and Lactobacillus fermentation effects on the isoflavones biotransformation and removal of anti-nutritional factors of soy bean", J Mar Sci Technol, 22, pp.525~530 (2014)[DOI]
- Handa CL, "Multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design", Food Chem, 197, pp.175~184 (2016)[DOI]
- Oyaizu M, "Studies on products of browning reaction : Antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)[DOI]
- GyamfiMA, "Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries", Gen Pharmacol, 32, pp.661~667 (1999)[DOI]
- Yuris A, "Comparative study of the antioxidant properties of three pineapple (Ananas comosus L. ) Varieties", J Food Stud, 3, pp.40~56 (2014)[DOI]
- NRC, "Nutrient requirements of poultry" (1994), National Academy Press
- Murugan M, "Broiler performance efficiency factor(BPEF)in commercial broiler production facilities with special reference to climate", Indian Vet J, 94, pp.11~14 (2017)
- Liu Z, "Bacillus velezensis DP-2 isolated from douchi and its application in soybean meal fermentation", J Sci Food Agric, 101, pp.1861~1868 (2020)[DOI]
- Zhu J, "Effects of soybean meal fermented by L. plantarum, B. subtilis and S. cerevisieae on growth, immune function and intestinal morphology in weaned piglets", Microb Cell Fact, 16, pp.191~ (2017)[DOI]
- Ketnawa S, "Comparative study on protein digestibility, protein patterns, antioxidant activities of raw, cooked and fermented soybeans", J Food Sci Agric Technol, 4(Specl Iss.), pp.7~16 (2018)
- Runni Mukherjee, "Role of Fermentation in Improving Nutritional Quality of Soybean Meal — A Review", Animal Bioscience, 29(11), pp.1523~1529 (2016), 아세아·태평양축산학회[DOI]
- Alikwe PCN, "Biochemical evaluation of serum metabolites, enzymes and haematological indices of broiler-chicks fed with varying levels of rumen epithelial scraps in place of fish meal proteins", Res J Poult Sci, 3, pp.27~31 (2010)[DOI]
- J. Apajalahti, "Interaction between chicken intestinal microbiota and protein digestion", Animal Feed Science and Technology, 221, pp.323~330 (2016), Elsevier BV[DOI]
- Soumeh EA, "Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens", Poult Sci, 98, pp.6797~6807 (2019)[DOI]
- Guo S, "Partial substitution of fermented soybean meal for soybean meal influences the carcass traits and meat", Animals, 10, pp.225~ (2020)[DOI]
- M.T. Lee, "Potential crosstalk of oxidative stress and immune response in poultry through phytochemicals — A review", Animal Bioscience, 32(3), pp.309~319 (2019), 아세아·태평양축산학회[DOI]
- Karaś M, "Influence of physiological and chemical factors on the absorption of bioactive peptides", Int J Food Sci Technol, 54, pp.1486~1496 (2019)[DOI]
- Ayyadurai S, "PepT1 expressed in immune cells has an important role in promoting the immune response during experimentally induced colitis", Lab Invest, 93, pp.888~899 (2013)[DOI]