Reversion of BDNF, Akt and CREB in Hippocampus of Chronic Unpredictable Stress Induced Rats: Effects of Phytochemical, Bacopa Monnieri
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: The aims of the present study were to explore the behavioural effects and to understand the possible mode of action of Bacopa monnieri extract (BME) on chronic unpredictable stress (CUS) induced depressive model and the biochemical alterations such as brain derived neurotrophic factor (BDNF), Akt, cyclic-AMP response element binding (CREB) protein level in the hippocampus of rats. Methods: We examined the effects of chronic administration of BME on CUS exposed rats for 28 days. Behavioural changes were assessed by sucrose consumption and open field test to assess the effect of BME on CUS-induced depression. The mechanisms underlying antidepressant like action of BME was further evaluated by measuring levels of BDNF, Akt, and CREB in the hippocampus of rat brain and compared with the standard tricyclic antidepressant drug imipramine (20 mg/kg body weight). Results: Exposure to CUS for 28 days produced depression-like behavior in rats, as indicated by significant decreases in sucrose consumption, locomotor activity including decreased BDNF, Akt and CREB levels in the hippocampus. Daily administration of BME at a dose of (80 mg/kg body weight) significantly reverses the behavioral alteration and restored the normal level of BDNF, total and phospho-Akt, total and phospho CREB in the hippocampus of CUS induced rats as compared to vehicle treated control rats. Conclusion: These findings suggest that BME ameliorates CUS induced behavioural depression in rats and that can be used as a potent therapeutic agent in treating depressive like behavior.
Abstract
Objective: The aims of the present study were to explore the behavioural effects and to understand the possible mode of action of Bacopa monnieri extract (BME) on chronic unpredictable stress (CUS) induced depressive model and the biochemical alterations such as brain derived neurotrophic factor (BDNF), Akt, cyclic-AMP response element binding (CREB) protein level in the hippocampus of rats. Methods: We examined the effects of chronic administration of BME on CUS exposed rats for 28 days. Behavioural changes were assessed by sucrose consumption and open field test to assess the effect of BME on CUS-induced depression. The mechanisms underlying antidepressant like action of BME was further evaluated by measuring levels of BDNF, Akt, and CREB in the hippocampus of rat brain and compared with the standard tricyclic antidepressant drug imipramine (20 mg/kg body weight). Results: Exposure to CUS for 28 days produced depression-like behavior in rats, as indicated by significant decreases in sucrose consumption, locomotor activity including decreased BDNF, Akt and CREB levels in the hippocampus. Daily administration of BME at a dose of (80 mg/kg body weight) significantly reverses the behavioral alteration and restored the normal level of BDNF, total and phospho-Akt, total and phospho CREB in the hippocampus of CUS induced rats as compared to vehicle treated control rats. Conclusion: These findings suggest that BME ameliorates CUS induced behavioural depression in rats and that can be used as a potent therapeutic agent in treating depressive like behavior.
참고문헌 (32)
- Shelton RC, "The molecular neurobiology of depression", Psychiatr Clin North Am, 30, pp.1~11 (2007)
- Kessler RC, "Prevalence, severity, and comorbidity of 12-month DSM-IV disorders in the National Comorbidity Survey Replication", Arch Gen Psychiatry, 62, pp.617~627 (2005)
- Ritabrata Banerjee, "Chronic Administration of Bacopa Monniera Increases BDNF Protein and mRNA Expressions: A Study in Chronic Unpredictable Stress Induced Animal Model of Depression", PSYCHIATRY INVESTIGATION, 11(3), pp.297~306 (2014), 대한신경정신의학회[DOI]
- Mao QQ, "Effects of SYJN, a Chinese herbal formula, on chronic unpredictable stress-induced changes in behavior and brain BDNF in rats", J Ethnopharmacol, 128, pp.336~341 (2010)
- Hazra S, "Bacopa monniera treatment reverses chronic unpredictable stress induced depressive like behavior by increasing expression of neurotrophins in rat brain", RJPBCS, 4, pp.775~786 (2013)
- Hazra S, "Chronic administration of bacopa monniera alleviates depressive like behavior and increases the expression of ERK1", 2 in hippocampus and pre frontal cortex of chronic unpredictable stress induced rats, 2015, pp.3, 47~58
- Zhang ZJ, "Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders", Life Sci, 75, pp.1659~1699 (2004)
- Calabrese C, "Effects of a standardized Bacopa monnieri extract on cognitive performance, anxiety, and depression in the elderly: a randomized, double-blind, placebo-controlled trial", J Altern Complement Med, 14, pp.707~713 (2008)
- Paulose CS, "Neuroprotective role of Bacopa monnieri extract in epilepsy and effect of glucose supplementation during hypoxia : glutamate receptor gene expression", Neurochem Res, 33, pp.1663~1671 (2008)
- Chatterjee M, "Comparative evaluation of Bacopa monniera and Panax quniquefolium in experimental anxiety and depressive models in mice", Ind J Exp Biol, 48, pp.306~313 (2010)
- Willner P, "Chronic mild stress(CMS)revisited : consistency and behavioural neurobiological concordance in the effects of CMS", Neuropsychobiology, 52, pp.90~110 (2005)
- Banerjee R, "Behavioral consequences of chronic stress and effects of antidepressant treatment on animal models of depression", Am J Psychiatry Neurosci, 1, pp.5~13 (2013)
- Kossel AH, "A caged Ab reveals an immediate", instructive effect of BDNF during hippocampal synaptic potentiation, 2001, pp.98, 14702~14707
- Duman RS, "A neurotrophic model for stress-related mood disorders", Biol Psychiatry, 59, pp.1116~1127 (2006)
- Yang J, "Neuronal release of pro BDNF", Nat Neurosci, 12, pp.113~115 (2009)
- Mattson MP, "Glutamate and neurotrophic factors in neuronal plasticity and disease", Ann N Y Acad Sci, 1144, pp.97~112 (2008)
- Yoshii A, "Postsynaptic BDNF-TrkB signaling in synapse maturation, plasticity, and disease", Dev Neurobiol, 70, pp.304~322 (2010)
- Luo DD, "Involvement of hippocampal serotonin and neuropeptide Y in depression induced by chronic unpredicted mild stress", Brain Res Bull, 77, pp.8~12 (2008)
- Li S, "Antidepressant like effects of piperine in chronic mild stress treated mice and its possible mechanisms", Life Sci, 80, pp.1373~1381 (2007)
- Zhao Z, "Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats", Behav Brain Res, 194, pp.108~113 (2008)
- Fuchs E, "Alterations of neuroplasticity in depression: the hippocampus and beyond", Eur Neuropsychopharmacol, 14(Suppl 5), pp.S481~S490 (2004)
- Bremner JD, "Hippocampal volume reduction in major depression", Am J Psychiatry, 157, pp.115~118 (2000)
- McEwen BS, "Stress effects on morphology and function of the hippocampus", Ann NY Acad Sci, 821, pp.271~284 (1997)
- Banerjee R, "Decreased mRNA and protein expressions of BDNF, NGF and their receptors in the hippocampus from suicide: an analysis in human postmortem brain", Clin Med Insights Pathol, 6, pp.1~11 (2013)
- Castrén E, "Role of neurotrophic factors in depression", Curr Opin Pharmacol, 7, pp.18~21 (2007)
- Castrén E, "The role of BDNF and its receptors in depression and antidepressant drug action : reactivation of developmental plasticity", Dev Neurobiol, 70, pp.289~297 (2010)
- Shirayama Y, "Brain derived neurotrophic factor produces antidepressant effects in behavioral models of depression", J Neurosci, 22, pp.3251~3261 (2002)
- Levine ES, "Brain-derived neurotrophic factor modulates hippocampal synaptic transmission by increasing N-methyl-D-aspartic acid receptor activity", Proc Natl Acad Sci U S A, 95, pp.10235~10239 (1998)
- Lu Y, "BDNF: a key regulator for protein synthesis dependent LTP and long-term memory?", Neurobiol Learn Mem, 89, pp.312~323 (2008)
- Qi XL, "A role for the extracellular signal-regulated kinase signal pathway in depressive-like behavior", Behav Brain Res, 199, pp.203~209 (2009)
- Qi XL, "Fluoxetine increases the activity of the ERK-CREB signal system and alleviates the depressive like behavior in rats exposed to chronic forced swim stress", Neurobiol Dis, 31, pp.278~285 (2008)
- Rantamäki T, "Pharmacologically diverse antidepressants rapidly activate brain-derived neurotrophic factor receptor TrkB and induce phospholipase-C gamma signaling pathways in mouse brain", Neuropsychopharmacology, 32, pp.2152~2162 (2007)
