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Assessment of the gastrointestinal microbiota using 16S ribosomal RNA gene amplicon sequencing in ruminant nutrition

김민석
아세아·태평양축산학회 (Asian-Australasian Association Of Animal Production Societies)|2023.02|Animal Bioscience|제36권 제2호|pp. 364-373|DOI 10.5713/ab.22.0382

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참고문헌

키워드

Microbiota16S rRNA GeneAmplicon SequencingGastrointestinal TractRuminant Nutrition

초록

The gastrointestinal (GI) tract of ruminants contains diverse microbes that ferment various feeds ingested by animals to produce various fermentation products, such as volatile fatty acids. Fermentation products can affect animal performance, health, and well-being. Within the GI microbes, the ruminal microbes are highly diverse, greatly contribute to fermentation, and are the most important in ruminant nutrition. Although traditional cultivation methods provided knowledge of the metabolism of GI microbes, most of the GI microbes could not be cultured on standard culture media. By contrast, amplicon sequencing of 16S rRNA genes can be used to detect unculturable microbes. Using this approach, ruminant nutritionists and microbiologists have conducted a plethora of nutritional studies, many including dietary interventions, to improve fermentation efficiency and nutrient utilization, which has greatly expanded knowledge of the GI microbiota. This review addresses the GI content sampling method, 16S rRNA gene amplicon sequencing, and bioinformatics analysis and then discusses recent studies on the various factors, such as diet, breed, gender, animal performance, and heat stress, that influence the GI microbiota and thereby ruminant nutrition.

Abstract

The gastrointestinal (GI) tract of ruminants contains diverse microbes that ferment various feeds ingested by animals to produce various fermentation products, such as volatile fatty acids. Fermentation products can affect animal performance, health, and well-being. Within the GI microbes, the ruminal microbes are highly diverse, greatly contribute to fermentation, and are the most important in ruminant nutrition. Although traditional cultivation methods provided knowledge of the metabolism of GI microbes, most of the GI microbes could not be cultured on standard culture media. By contrast, amplicon sequencing of 16S rRNA genes can be used to detect unculturable microbes. Using this approach, ruminant nutritionists and microbiologists have conducted a plethora of nutritional studies, many including dietary interventions, to improve fermentation efficiency and nutrient utilization, which has greatly expanded knowledge of the GI microbiota. This review addresses the GI content sampling method, 16S rRNA gene amplicon sequencing, and bioinformatics analysis and then discusses recent studies on the various factors, such as diet, breed, gender, animal performance, and heat stress, that influence the GI microbiota and thereby ruminant nutrition.

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관련 논문AI추천

— Invited Review — Metagenomic investigation of gastrointestinal microbiome in cattle김민석, 박탄솔, Zhongtang Yu아세아·태평양축산학회 · Animal Bioscience2017 · 제30권 제11호Understanding the functionality of the rumen microbiota: searching for better opportunities for rumen microbial manipulationJia Ming-Hui, Qi Wenlingli, Sun Hui-Zeng, Xue Ming-Yuan, Yan Qiongxian, Zhang Shuxian아세아·태평양축산학회 · Animal Bioscience2024 · 제37권 제2호Relationship between the structure and composition of rumen microorganisms and the digestibility of neutral detergent fibre in goatsBai Xue, Kaizhen Liu, Lizhi Wang, Quanhui Peng, Tianhai Yan, Zhisheng Wang아세아·태평양축산학회 · Animal Bioscience2019 · 제32권 제1호Translational gut microbiome research for strategies to improve beef cattle production sustainability and meat qualityGuan Le Luo, Mizoguchi Yasushi아세아·태평양축산학회 · Animal Bioscience2024 · 제37권 제2호Effects of Sampling Techniques and Sites on Rumen Microbiome and Fermentation Parameters in Hanwoo Steers이슬, 최혁, 김민석, 백율창, 송재용, 이현정, 정진영, 지상윤한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제10호The impact of diet on the composition and relative abundance of rumen microbes in goatJiwen Wang, Kaizhen Liu, Lizhi Wang, Meili Zhou, Qin Xu, Wei Guo아세아·태평양축산학회 · Animal Bioscience2017 · 제30권 제4호

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