본문 바로가기
KERD
논문KCI 등재

Genome‑wide identification, organization, and expression profiles of the chicken fibroblast growth factor genes in public databases and Vietnamese indigenous Ri chickens against highly pathogenic avian influenza H5N1 virus infection

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen‑activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R2 = 0.92– 0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.

Abstract

Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen‑activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R2 = 0.92– 0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.

참고문헌 (38)

  1. Samuel O. Olapoju, "Fibroblast growth factor 21; review on its participation in vascular calcification pathology", Vascular Pharmacology, 125-126, pp.106636~ (2020), Elsevier BV[DOI]
  2. Forough R, "Role of AKT", PKB signaling in fibroblast growth factor-1(FGF-1)-induced angiogenesis in the chicken chorioallantoic membrane(CAM), 2005, pp.94, 109~116
  3. Choi JW, "Basic fibroblast growth factor activates MEK", ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells, 2010, pp.5, e12968~
  4. Wu YF, "C-Type natriuretic peptide dampens fibroblast growth factor-23 expression through MAPK signaling pathway in human mesangial cells", J Interferon Cytokine Res, 38, pp.500~509 (2018)[DOI]
  5. Wang X, "Feedback activation of basic fibroblast growth factor signaling via the wnt", beta-catenin pathway in skin fibroblasts, 2017, pp.8, 32~
  6. Li S, "Fibroblast growth factor 21ameliorates high glucose-induced fibrogenesis in mesangial cells through inhibiting STAT5 signaling pathway", Biomed Pharmacother, 93, pp.695~704 (2017)[DOI]
  7. Yang PH, "Human basic fibroblast growth factor inhibits tau phosphorylation via the PI3K", Akt-GSK3beta signaling pathway in a 6-hydroxydopamineinduced model of Parkinson's disease, 2016, pp.16, 357~369
  8. Borland CZ, "Fibroblast growth factor signaling in Caenorhabditis elegans", Bioessays, 23, pp.1120~1130 (2001)[DOI]
  9. Duszynski RJ, "Divergent requirements for fibroblast growth factor signaling in zebrafish maxillary barbel and caudal fin regeneration", Dev Growth Differ, 55, pp.282~300 (2013)[DOI]
  10. Zhan DC, "Efficacy and safety of basic fibroblast growth factor in the treatment of burns : Protocol for a systematic review and meta-analysis of randomized controlled trials", Medicine(Baltimore), 98, pp.e15102~ (2019)[DOI]
  11. Gallegos TF, "Fibroblast growth factor signaling mediates progenitor cell aggregation and nephron regeneration in the adult zebrafish kidney", Dev Biol, 454, pp.44~51 (2019)[DOI]
  12. Schofer C, "The apical ectodermal ridge, fibroblast growth factors(FGF-2 and FGF-4)and insulin-like growth factor I(IGF-I)control the migration of epidermal melanoblasts in chicken wing buds", Anat Embryol(Berl), 203, pp.137~146 (2001)[DOI]
  13. Dono R, "Cell-type-specific nuclear translocation of fibroblast growth factor-2 isoforms during chicken kidney and limb morphogenesis", Dev Biol, 163, pp.316~330 (1994)[DOI]
  14. Wang M, "Regulation of fibroblast growth factor 8(FGF8)in chicken embryonic stem cells differentiation into spermatogonial stem cells", J Cell Biochem, 119, pp.2396~2407 (2018)[DOI]
  15. Munoz-Sanjuan I, "Expression of chicken fibroblast growth factor homologous factor(FHF)-1 and of differentially spliced isoforms of FHF-2 during development and involvement of FHF-2 in chicken limb development", Development, 126, pp.409~421 (1999)[DOI]
  16. Kurose H, "Expression of Fibroblast growth factor 19(Fgf19)during chicken embryogenesis and eye development, compared with Fgf15 expression in the mouse", Gene Expr Patterns, 4, pp.687~693 (2004)[DOI]
  17. Frohns F, "Basic fibroblast growth factor increases the precursor pool of photoreceptors, but inhibits their differentiation and apoptosis in chicken retinal reaggregates", Eur J Neurosci, 29, pp.1931~1942 (2009)[DOI]
  18. Kent WJ, "BLAT--the BLAST-like alignment tool", Genome Res, 12, pp.656~664 (2002)[DOI]
  19. Bailey TL, "MEME SUITE : tools for motif discovery and searching", Nucleic Acids Res, 37, pp.W202~ (2009)[DOI]
  20. Tamura K, "MEGA6 : molecular evolutionary genetics analysis version 6. 0", Mol Biol Evol, 30, pp.2725~2729 (2013)[DOI]
  21. Bui VN, "Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam", Virus Genes, 49, pp.278~285 (2014)[DOI]
  22. OIE, "Chapter 3. 3. 4 : Avian influenza(infection with avian influenza viruses)(NB : Version adopted in May 2015)", OIE, ~ (2018)
  23. Reed LJ, "A simple method of estimating fifty per cent endpoints", Am J Epidemiol, 27, pp.493~497 (1938)[DOI]
  24. 부 티 하오, "Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines", Animal Bioscience, 35(3), pp.367~376 (2022), 아세아·태평양축산학회[DOI]
  25. Young Sun Hwang, "The transcriptome of early chicken embryo reveal signaling pathways governing rapid asymmetric cellularization and lineage segregation", Development (2018), The Company of Biologists[DOI]
  26. Trapnell C, "Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation", Nat Biotechnol, 28, pp.511~515 (2010)[DOI]
  27. Szklarczyk D, "The STRING database in 2017 : quality-controlled protein-protein association networks, made broadly accessible", Nucleic Acids Res, 45, pp.D362~ (2017)[DOI]
  28. Kenneth J. Livak, "Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method", Methods, 25(4), pp.402~408 (2001), Elsevier BV[DOI]
  29. Ruiz-Narvaez EA, "Gene-based analysis of the fibroblast growth factor receptor signaling pathway in relation to breast cancer in African American women : the AMBER consortium", Breast Cancer Res Treat, 155, pp.355~363 (2016)[DOI]
  30. Jiang L, "Genome-wide identification, phylogeny, and expression of fibroblast growth genes in common carp", Gene, 578, pp.225~231 (2016)[DOI]
  31. Bagchi M, "Heat shock proteins of chicken lens", J Cell Biochem, 82, pp.409~414 (2001)[DOI]
  32. Munaim SI, "Developmental changes in fibroblast growth factor in the chicken embryo limb bud", Proc Natl Acad Sci USA, 85, pp.8091~8093 (1988)[DOI]
  33. Consigli SA, "Immunolocalization of basic fibroblast growth factor during chicken cardiac development", J Cell Physiol, 146, pp.379~385 (1991)[DOI]
  34. Mattoo RL, "The roles of fibroblast growth factor(FGF)-23, alpha-klotho and furin protease in calcium and phosphate homeostasis : a mini-review", Indian J Clin Biochem, 29, pp.8~12 (2014)[DOI]
  35. Chen G, "The fibroblast growth factor receptor 2-mediated extracellular signal-regulated kinase 1", 2 signaling pathway plays is important in regulating excision repair cross-complementary gene 1 expression in hepatocellular carcinoma, 2013, pp.1, 604~608
  36. Jeong W, "Fibroblast growth factor 4-induced migration of porcine trophectoderm cells is mediated via the AKT cell signaling pathway", Mol Cell Endocrinol, 419, pp.208~216 (2016)[DOI]
  37. Charoenngam N, "Oral vitamin D3 supplementation increases serum fibroblast growth factor 23 concentration in vitamin D-deficient patients : a systematic review and meta-analysis", Osteoporos Int, 30, pp.2183~2193 (2019)[DOI]
  38. Tsai WC, "Effects of lower versus higher phosphate diets on fibroblast growth factor-23 levels in patients with chronic kidney disease : a systematic review and metaanalysis", Nephrol Dial Transplant, 33, pp.1977~1983 (2018)[DOI]

관련 논문AI추천

The highly pathogenic H5N1 avian influenza virus induces the mitogen-activated protein kinase signaling pathway in the trachea of two Ri chicken lines부 티 하오, 홍영호, Heo Jubi, Hong Yeojin, Lee Sooyeon, Lillehoj,Hyun S., Truong Anh Duc아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제7호MicroRNA expression profiling in the lungs of genetically different Ri chicken lines against the highly pathogenic avian influenza H5N1 virus이수연, 허주비, 홍여진, 홍영호, Anh Duc Truong, Hyun S. Lillehoj, Suyeon Kang, Thi Hao Vu한국축산학회 · 한국축산학회지2023 · 제65권 제4호Changes in Apoptosis-related Gene Expression Induced by Repression of FGFR1 by RNA Interference in Embryonic Fibroblasts and Cancerous Cells from Chicken이보람, 이상인, 한재용, 황영선, 데이벤드란렝가라즈한국축산학회 · 한국축산학회지2010 · 제52권 제6호Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines부 티 하오, 송기덕, 홍영호, 츠엉앵득, Cha Jihye, Dang Hoang Vu, Hong Yeojin, Lee Jiae, Lee Sooyeon, Lillehoj,Hyun S., Tran Ha Thi Thanh아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제3호Genetic insights into avian influenza resistance in Jeju Island chickens: the roles of Mx1 and oligoadenylate synthetase-like single nucleotide polymorphisms서현정, Dong-Seob Tark, Eun-A Ko, Hyoung-Seok Yang, Jae-Hong Ko, Ju-Hee Yang, Seong Hwan Mun, Sung Hyun Kang, Woo Hyun Kim, Young-Won Kim한국축산학회 · 한국축산학회지2025 · 제67권 제1호Molecular characterization and expression of a disintegrin and metalloproteinase with thrombospondin motifs 8 in chicken김성조, 송기덕, 이라함, 이석현, 이학교, Yu Ra Kim아세아·태평양축산학회 · Animal Bioscience2018 · 제31권 제8호

이 간행물의 다른 논문 (12건)