본문 바로가기
KERD
논문KCI 등재

Effects of gold and green kiwifruit juices on the physicochemical properties and tenderness of pork loin and antioxidant activity during incubation (24 h) in a pork model system

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: Although pork loins is not a tough meat, they need to develop meat products with a soft texture for the elderly. This study focused on the physicochemical properties and tenderness characteristics of pork loin injected with green kiwifruit juice (GRJ) and gold kiwifruit juice (GOJ) during various incubation times. In addition, the antioxidant activities of hydrolysate derived from the hydrolysis of pork loin by kiwifruit juice protease were evaluated.Methods: The pork loin was injected with 10% and 20% GRJ and GOJ, under various incubation times (0, 4, 8, and 24 h). Then, the physicochemical properties and tenderness of pork loins were measured. 2,2- diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power were conducted to determine hydrolysate's antioxidant activities derived from pork loin's hydrolysis by kiwifruit juice protease.Results: GRJ had greater tenderizing ability than GOJ, even at the 10% addition. When kiwifruit juice was injected into pork loin, the tenderness increased with increasing incubation time. This was confirmed by the decrease in intensity of the myosin heavy chain (MHC) band in sodium dodecyl sulfate–polyacrylamide gel electrophoresis. In particular, the MHC band decreased at 8 h for both 10% GRJ and 20% GOJ and at 4 h for 20% GRJ alone. The highest myofibril fragmentation index and peptide solubility were observed in pork loin treated with 20% GRJ compared to the other treatments during incubation. The 10% GRJ and 20% GOJ treatments showed similar levels of antioxidant activity of the protein hydrolysates in pork loin, and 20% GRJ showed the highest activity among the treatments.Conclusion: Kiwifruit juice had protease activity, and GRJ was more useful for tenderizing meat products than GOJ. Thus, GRJ at 10% could be a potential agent to tenderize and enrich the natural antioxidant activity through the proteolysis of pork loin.

Abstract

Objective: Although pork loins is not a tough meat, they need to develop meat products with a soft texture for the elderly. This study focused on the physicochemical properties and tenderness characteristics of pork loin injected with green kiwifruit juice (GRJ) and gold kiwifruit juice (GOJ) during various incubation times. In addition, the antioxidant activities of hydrolysate derived from the hydrolysis of pork loin by kiwifruit juice protease were evaluated. Methods: The pork loin was injected with 10% and 20% GRJ and GOJ, under various incubation times (0, 4, 8, and 24 h). Then, the physicochemical properties and tenderness of pork loins were measured. 2,2- diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power were conducted to determine hydrolysate's antioxidant activities derived from pork loin's hydrolysis by kiwifruit juice protease. Results: GRJ had greater tenderizing ability than GOJ, even at the 10% addition. When kiwifruit juice was injected into pork loin, the tenderness increased with increasing incubation time. This was confirmed by the decrease in intensity of the myosin heavy chain (MHC) band in sodium dodecyl sulfate–polyacrylamide gel electrophoresis. In particular, the MHC band decreased at 8 h for both 10% GRJ and 20% GOJ and at 4 h for 20% GRJ alone. The highest myofibril fragmentation index and peptide solubility were observed in pork loin treated with 20% GRJ compared to the other treatments during incubation. The 10% GRJ and 20% GOJ treatments showed similar levels of antioxidant activity of the protein hydrolysates in pork loin, and 20% GRJ showed the highest activity among the treatments. Conclusion: Kiwifruit juice had protease activity, and GRJ was more useful for tenderizing meat products than GOJ. Thus, GRJ at 10% could be a potential agent to tenderize and enrich the natural antioxidant activity through the proteolysis of pork loin.

참고문헌 (30)

  1. Ahmad MN, "Optimization of a protease extraction using a statistical approach for the production of an alternative meat tenderizer from Spondias cytherea roots", J Food Process Preserv, 43, pp.e14192~ (2019)[DOI]
  2. Rawdkuen S, "Physicochemical properties and tenderness of meat samples using proteolytic extract from Calotropis procera latex", Food Chem, 136, pp.909~916 (2013)[DOI]
  3. Toohey ES, "The effect of a kiwi fruit based solution on meat traits in beef m. semimembranosus(topside)", Meat Sci, 88, pp.468~471 (2011)[DOI]
  4. Zhu X, "Actinidin pretreatment and sous vide cooking of beef brisket : effects on meat microstructure, texture and in vitro protein digestibility", Meat Sci, 145, pp.256~265 (2018)[DOI]
  5. Boland M, "Kiwifruit proteins and enzymes : actinidin and other significant proteins", Adv Food Nutr Res, 68, pp.59~80 (2013)[DOI]
  6. Ha M, "Characterisation of commercial papain, bromelain, actinidin and zingibain protease preparations and their activities toward meat proteins", Food Chem, 134, pp.95~105 (2012)[DOI]
  7. Gong X, "Comparative efficacy of actinidin from green and gold kiwi fruit extract on in vitro simulated protein digestion of beef Semitendinosus and its myofibrillar protein fraction", Int J Food Sci Technol, 55, pp.742~750 (2020)[DOI]
  8. Udenigwe CC, "Meat proteome as source of functional biopeptides", Food Res Int, 54, pp.1021~1032 (2013)[DOI]
  9. Zhang B, "Characterization of actinidin from Chinese kiwifruit cultivars and its applications in meat tenderization and production of angiotensin I-converting enzyme(ACE)inhibitory peptides", LWT, 78, pp.1~7 (2017)[DOI]
  10. Saiga AI, "Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment", J Agric Food Chem, 51, pp.3661~3667 (2003)[DOI]
  11. Kaur L, "Actinidin enhances gastric protein digestion as assessed using an in vitro gastric digestion model", J Agric Food Chem, 58, pp.5068~5073 (2010)[DOI]
  12. Rakasivi KGJ, "Evaluation of product quality of chicken sausages with added cinnamon(Cinnamomum cassia)and mushroom(Pleurotus sajur-caju)powders at various concentrations", J Food Process Preserv, 46, pp.e16958~ (2022)[DOI]
  13. Wheeler TL, "Relationship of beef longissimus tenderness classes to tenderness of gluteus medius, semimembranosus, and biceps femoris", J Anim Sci, 78, pp.2856~2861 (2000)[DOI]
  14. Culler RD, "Relationship of myofibril fragmentation index to certain chemical, physical and sensory characteristics of bovine longissimus muscle", J Food Sci, 43, pp.1177~1180 (1978)[DOI]
  15. Haga S, "Heat-induced gelation of a mixture of myosin B and soybean protein", Agric Biol Chem, 48, pp.1001~1007 (1984)[DOI]
  16. Zhang S, "Effect of flavourzyme on proteolysis, antioxidant activity and sensory qualities of Cantonese bacon", Food Chem, 237, pp.779~785 (2017)[DOI]
  17. Huang YC, "Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan", Food Chem, 98, pp.529~538 (2006)[DOI]
  18. Kaur L, "Actinidin in green and sungold kiwifruit improves digestion of alternative proteins—an in vitro investigation", Foods, 11, pp.2739~ (2022)[DOI]
  19. Chao D, "Actinidin: the predominant protease in kiwifruit: a thesis presented in partial fulfilment of the requirements", pp.2016
  20. Liu C, "Kiwifruit protease extract injection reduces toughness of pork loin muscle induced by freeze–thaw abuse", LWT-Food Sci Technol, 44, pp.2026~2031 (2011)[DOI]
  21. McGhie TK, "Color in fruit of the genus Actinidia : carotenoid and chlorophyll compositions", J Agric Food Chem, 50, pp.117~121 (2002)[DOI]
  22. Leygonie C, "Oxidative stability of previously frozen ostrich Muscularis iliofibularis packaged under different modified atmospheric conditions", Int J Food Sci Technol, 46, pp.1171~1178 (2011)[DOI]
  23. Chang JH, "Synergistic effect of sous-vide and fruitextracted enzymes on pork tenderization", Food Sci Biotechnol, 29, pp.1213~1222 (2020)[DOI]
  24. Purslow PP, "The structural basis of cooking loss in beef : variations with temperature and ageing", Food Res Int, 89, pp.739~748 (2016)[DOI]
  25. Bagheri Kakash S, "Kinetic study of the effect of kiwi fruit actinidin on various proteins of chicken meat", Food Sci Technol, 39, pp.980~992 (2019)[DOI]
  26. Davey CL, "Studies in meat tenderness. 7. Changes in the fine structure of meat during aging", J Food Sci, 34, pp.69~74 (1969)[DOI]
  27. Hopkins DL, "A research note on factors affecting the determination of myofibrillar fragmentation", Meat Sci, 56, pp.19~22 (2000)[DOI]
  28. Zhao G, "Enzymatic hydrolysis and their effects on conformational and functional properties of peanut protein isolate", Food Chem, 127, pp.1438~1443 (2011)[DOI]
  29. Teh SS, "Antioxidant and ACE-inhibitory activities of hemp(Cannabis sativa L. )protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain", Food Chem, 203, pp.199~206 (2016)[DOI]
  30. Yu D, "Protein degradation and peptide formation with antioxidant activity in pork protein extracts inoculated with Lactobacillus plantarum and Staphylococcus simulans", Meat Sci, 160, pp.107958~ (2020)[DOI]

관련 논문AI추천

Effects of injection of hydrolysis plasma protein solution on the antioxidant properties in porcine M. Longissimus Lumborum서진규, 서현우, 양한술한국축산학회 · 한국축산학회지2016 · 제58권 제8호과육(파인애플, 골드키위) 첨가 마리네이드에 의한 품종별 돈육 등심의 품질 특성 및 Di-peptide 함량변화김동욱, 김혜진, 김희진, 장애라, 전기홍동물생명과학연구소 · 동물자원연구2016 · 제27권 제3호Effect of Bovine Plasma Protein Hydrolysates on the Quality Properties of Cooked Pork Patty서진규, 서현우, 양한술, 염현웅농업생명과학연구원 · 농업생명과학연구2016 · 제50권 제1호Development of semi-dried goat meat jerky using tenderizers considering the preferences of the elderlyJi-Young Park, Ki Chang Nam, Md. Altaf Hossain, Shine Htet Aung, Young-Sun Choi한국축산학회 · 한국축산학회지2024 · 제66권 제4호후숙시기 동안 참다래의 품질, 기능성 및 액티니딘 함량 변화 조사남승희한국식품저장유통학회 · Food Science and Preservation2016 · 제23권 제3호Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goatsAhmed A. Abubakar, Awis Q. Sazili, Azad B. Sabow, Kazeem D. Adeyemi, Rafiat M. Shittu, Roselina Karim, Saiful A. Karsani한국축산학회 · 한국축산학회지2016 · 제58권 제6호

이 간행물의 다른 논문 (15건)