본문 바로가기
KERD
논문KCI 등재

Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits.Methods: A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T<sub>0</sub> (0 g/kg), T<sub>1</sub> (0.35 g/kg), T<sub>2</sub> (0.7 g/kg) for 28 d.Results: The results demonstrated that T<sub>2</sub> decreased the average daily gain (p<0.05). T<sub>1</sub> and T<sub>2</sub> decreased villus height and inflammatory factor levels as compared with T<sub>0</sub> (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T<sub>1</sub> were higher than those in T<sub>0</sub> and T<sub>2</sub> (p<0.05). The content of butyric acid in T<sub>2</sub> was lower than it in T<sub>0</sub> and T<sub>1</sub> (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T<sub>2</sub> significantly increased the abundance of <i>dgA-11_gut_group</i> and <i>Christensenellaceae_R-7_group</i> while decreased <i>Bacteroide</i> and <i>Ralstonia</i> as compared with T<sub>0</sub> (p<0.05). Compared with T<sub>0</sub>, T<sub>1</sub>, and T<sub>2</sub> significantly improved the gene expression of <i>JAM2</i>, <i>JAM3</i>, <i>mucin4</i>, <i>mucin6</i> (p<0.05), T<sub>1</sub> significantly decreased the expression of occluding while T<sub>2</sub> significantly increased (p<0.05), T<sub>2</sub> significantly increased the expression of <i>claudin1</i> and <i>claudin2</i> (p<0.05).Conclusion: DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.

Abstract

Objective: The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits. Methods: A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d. Results: The results demonstrated that T2 decreased the average daily gain (p<0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (p<0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (p<0.05). Compared with T0, T1, and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (p<0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (p<0.05), T2 significantly increased the expression of claudin1 and claudin2 (p<0.05). Conclusion: DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.

참고문헌 (44)

  1. Kim SW, "Meeting global feed protein demand : challenge, opportunity, and strategy", Annu Rev Anim Biosci, 7, pp.221~243 (2019)[DOI]
  2. Mottet A, "Livestock: on our plates or eating at our table? a new analysis of the feed", food debate, 2017, pp.14, 1~8
  3. Saddul D, "The potential of mulberry(Morus alba)as a fodder crop : the effect of plant maturity on yield, persistence and nutrient composition of plant fractions", Asian-Australas J Anim Sci, 17, pp.1657~1662 (2004)[DOI]
  4. Ding Y, "Effects of feeding fermented mulberry leaf powder on growth performance, slaughter performance, and meat quality in chicken broilers", Animals, 11, pp.3294~ (2021)[DOI]
  5. Fan L, "Morus nigra L. leaves improve the meat quality in finishing pigs", J Anim Physiol Anim Nutr(Berl), 104, pp.1904~1911 (2020)[DOI]
  6. Martínez M, "Feeding mulberry leaves to fattening rabbits : effects on growth, carcass characteristics and meat quality", Anim Sci, 80, pp.275~280 (2005)[DOI]
  7. Li YG, "1-Deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice", Sci Rep, 3, pp.1377~ (2013)[DOI]
  8. Samulitis BK, "Inhibitory mechanism of acarbose and 1-deoxynojirimycin derivatives on carbohydrases in rat small intestine", Drugs Exp Clin Res, 13, pp.517~524 (1987)
  9. Jiang L, "1-Deoxynojirimycin attenuates septic cardiomyopathy by regulating oxidative stress, apoptosis, and inflammation via the JAK2", STAT6 signaling pathway, 2022, pp.155, 113648~
  10. Piao X, "1-Deoxynojirimycin(DNJ)ameliorates indomethacin-induced gastric ulcer in mice by affecting NFkappaB signaling pathway", Front Pharmacol, 9, pp.372~ (2018)[DOI]
  11. Hu TG, "Effect of 1-deoxynojirimycin isolated from mulberry leaves on glucose metabolism and gut microbiota in a streptozotocin-induced diabetic mouse model", J Nat Prod, 82, pp.2189~2200 (2019)[DOI]
  12. Zheng J, "1-Deoxynojirimycin improves high fat diet-induced nonalcoholic steatohepatitis by restoring gut dysbiosis", J Nutr Biochem, 71, pp.16~26 (2019)[DOI]
  13. Hou QR, "Effects of dietary supplement of mulberry leaf(Morus alba)on growth and meat quality in rabbits", Indian J Anim Res, 54, pp.317~321 (2020)[DOI]
  14. Zhang DY, "Evaluation of the alkaloid, polyphenols, and antioxidant contents of various mulberry cultivars from different planting areas in eastern China", Ind Crops Prod, 122, pp.298~307 (2018)[DOI]
  15. National Research Council, "Nutrient requirements of rabbits" (1977), The National Academies Press
  16. Pérez J, "European reference method for in vivo determination of diet digestibility in rabbits", World Rabbit Sci, 3, pp.41~43 (1995)[DOI]
  17. Nakyinsige K, "Influence of gas stunning and halal slaughter(no stunning)on rabbits welfare indicators and meat quality", Meat Sci, 98, pp.701~708 (2014)[DOI]
  18. AOAC, "Official methods of analysis" (1990), AOAC Inc
  19. Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)[DOI]
  20. Martin-Gallausiaux C, "SCFA : mechanisms and functional importance in the gut", Proc Nutr Soc, 80, pp.37~49 (2021)[DOI]
  21. Habib H, "Plant protease inhibitors : a defense strategy in plants", Biotechnol Mol Biol Rev, 2, pp.68~85 (2007)
  22. Srivastava S, "Nutritional quality of leaves of some genotypes of mulberry(Morus alba)", Int J Food Sci Nutr, 57, pp.305~313 (2006)[DOI]
  23. Asano N, "Polyhydroxylated alkaloids isolated from mulberry trees(Morusalba L. )and silkworms(Bombyx mori L. )", J Agric Food Chem, 49, pp.4208~4213 (2001)[DOI]
  24. Yatsunami K, "The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars(Morus spp. )in Kyoto, Japan", J Nat Med, 62, pp.63~66 (2008)[DOI]
  25. Kim J, "Central administration of 1-deoxynojirimycin attenuates hypothalamic endoplasmic reticulum stress and regulates food intake and body weight in mice with high-fat diet-induced obesity", Evid Based Complement Alternat Med, 2017, pp.3607089~ (2017)[DOI]
  26. Hou Q, "1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese", Poult Sci, 99, pp.5858~5866 (2020)[DOI]
  27. Ma B, "Heat stress alters muscle protein and amino acid metabolism and accelerates liver gluconeogenesis for energy supply in broilers", Poult Sci, 100, pp.215~223 (2021)[DOI]
  28. Remppis S, "Effects of energy intake on performance, mobilization and retention of body tissue, and metabolic parameters in dairy cows with special regard to effects of pre-partum nutrition on lactation-a review", Asian-Australas J Anim Sci, 24, pp.540~572 (2011)[DOI]
  29. Song M, "Mulberry leaf extract improves intestinal barrier function and displays beneficial effects on colonic microbiota and microbial metabolism in weaned piglets", J Sci Food Agric, 103, pp.1561~1568 (2023)[DOI]
  30. Abdelqader A, "Effect of dietary butyric acid on performance, intestinal morphology, microflora composition and intestinal recovery of heat-stressed broilers", Livest Sci, 183, pp.78~83 (2016)[DOI]
  31. Guilloteau P, "From the gut to the peripheral tissues : the multiple effects of butyrate", Nutr Res Rev, 23, pp.366~384 (2010)[DOI]
  32. Reilly KJ, "Colonic short chain fatty acids mediate jejunal growth by increasing gastrin", Gut, 37, pp.81~86 (1995)[DOI]
  33. Tedelind S, "Anti-inflammatory properties of the short-chain fatty acids acetate and propionate : a study with relevance to inflammatory bowel disease", World J Gastroenterol, 13, pp.2826~2832 (2007)[DOI]
  34. Sun C, "Mulberry(Morus atropurpurea Roxb. )leaf protein hydrolysates ameliorate dextran sodium sulfate-induced colitis via integrated modulation of gut microbiota and immunity", J Funct Foods, 84, pp.104575~ (2021)[DOI]
  35. Sun X, "L-arginine and N-carbamoylglutamic acid supplementation enhance young rabbit growth and immunity by regulating intestinal microbial community", Asian-Australas J Anim Sci, 33, pp.166~176 (2020)[DOI]
  36. Goodrich JK, "Human genetics shape the gut microbiome", Cell, 159, pp.789~799 (2014)[DOI]
  37. Wang X, "Effects of high fructose corn syrup on intestinal microbiota structure and obesity in mice", NPJ Sci Food, 6, pp.17~ (2022)[DOI]
  38. Mancabelli L, "Identification of universal gut microbial biomarkers of common human intestinal diseases by meta-analysis", FEMS Microbiol Ecol, 93, pp.fix153~ (2017)[DOI]
  39. Bäuerl C, "Changes in cecal microbiota and mucosal gene expression revealed new aspects of epizootic rabbit enteropathy", PLoS One, 9, pp.e105707~ (2014)[DOI]
  40. Jin DX, "The underlying microbial mechanism of epizootic rabbit enteropathy triggered by a low fiber diet", Sci Rep, 8, pp.12489~ (2018)[DOI]
  41. Green HD, "Ralstonia infection in cystic fibrosis", Epidemiol Infect, 145, pp.2864~2872 (2017)[DOI]
  42. Hartsock A, "Adherens and tight junctions : structure, function and connections to the actin cytoskeleton", Biochim Biophys Acta Biomembr, 1778, pp.660~669 (2008)[DOI]
  43. Liu Y, "Human junction adhesion molecule regulates tight junction resealing in epithelia", J Cell Sci, 113, pp.2363~2374 (2000)[DOI]
  44. Burgueño JF, "Epithelial toll-like receptors and their role in gut homeostasis and disease", Nat Rev Gastroenterol Hepatol, 17, pp.263~278 (2020)[DOI]

관련 논문AI추천

Incorporation of unfermented or fermented de-oiled rice bran meal into a rabbit’s diet impacts growth performance, nutrient digestibility, cecal microbiota composition, and intestinal barrier functionDaniel Sindaye, Hang Suqin, Jinghong Liao, Zeid Shehata아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제7호Effects of a multi-strain probiotic on productive traits, antioxidant defence, caecal microbiota and short‑chain fatty acid profile, and intestinal histomorphology in rabbitsIarussi Fabrizio, Laudadio Vito, Losacco Caterina, Passantino Letizia, Pugliese Gianluca, Schiavitto Michele, Tateo Alessandra, Tufarelli Vincenzo아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제6호Effect of Salvia miltiorrhiza aerial parts on growth performance, nutrient digestibility, and digestive enzymes in rabbitsLi Pei, Liu Ning, Luo Yiran, Wang Jiajia, Zhang Feike아세아·태평양축산학회 · Animal Bioscience2021 · 제34권 제12호Effects of dietary mulberry leaves on growth, production performance, gut microbiota, and immunological parameters in poultry and livestock: a systematic review and meta-analysisCheng Hongbing, Gao Jinbo, Geng Bing, Guo Guang, Wang Zhaohong아세아·태평양축산학회 · Animal Bioscience2024 · 제37권 제6호Effects of yeast hydrolysate supplementation on intestinal morphology, barrier, and anti-inflammatory functions of broilersCheng Kang, Li QiMing, Wang Tian, Wang Ting아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제6호Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned pigletsBolin Zhang, Feng Gao, Guanghong Zhou, Jiaolong Li, Lin Zhang, Meng Lin, Ping Zhou, Shen Xing아세아·태평양축산학회 · Animal Bioscience2017 · 제30권 제2호

이 간행물의 다른 논문 (16건)