Novel polymorphisms of dopa decarboxylase gene and their association with lamb quality traits in Indonesian sheep
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Objective: This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep. Methods: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR. Results: The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits. Conclusion: The DDC gene could be used as a potential candidate gene to improve lamb quality.
Abstract
Objective: This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep. Methods: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR. Results: The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits. Conclusion: The DDC gene could be used as a potential candidate gene to improve lamb quality.
참고문헌 (36)
- "Organisation for Economic Co-Operation and Development-Food and Agriculture Organization on The United Nations" (2022)[URL]
- Dismas S. Shija, "Chemical Composition and Meat Quality Attributes of Indigenous Sheep and Goats from Traditional Production System in Tanzania", Animal Bioscience, 26(2), pp.295~302 (2013), 아세아·태평양축산학회[DOI]
- Listyarini K, "Association and expression of CYP2A6 AND KIF12 genes related to lamb flavour and odor", Trop Anim Sci J, 41, pp.100~107 (2018)[DOI]
- Van Elswyk ME, "Impact of grass", forage feeding versus grain finishing on beef nutrients and sensory quality : The U. S. experience, 2014, pp.96, 535~540
- Damian Frank, "Consumer Acceptability of Intramuscular Fat", 한국축산식품학회지, 36(6), pp.699~708 (2016), 한국축산식품학회
- Kyselova J, "The role of molecular genetics in animal breeding : a minireview", Czech J Anim Sci, 66, pp.107~111 (2021)[DOI]
- Gunawan A, "Hepatic transcriptome analysis identifies genes, polymorphisms and pathways involved in the fatty acids metabolism in sheep", PLoS One, 16, pp.e0260514~ (2021)[DOI]
- R S Harahap, "Effect of CYP2E1 gene polymorphisms on lamb odor and flavor in Indonesian sheep", IOP Conference Series: Earth and Environmental Science, 788(1), pp.012022~ (2021), IOP Publishing[DOI]
- Azizah D, "Investigation of leptin receptor(LEPR)gene polymorphism and association with carcass characteristic and meat quality in sheep", JIPTHP, 8, pp.124~130 (2020)[DOI]
- Wang X, "Proteomic analyses of sheep(ovis aries)embryonic skeletal muscle", Sci Rep, 10, pp.1750~ (2020)[DOI]
- Kojima K, "Gene therapy improves motor and mental function of aromatic l-amino acid decarboxylase deficiency", Brain, 142, pp.322~333 (2019)[DOI]
- Himmelreich N, "Aromatic amino acid decarboxylase deficiency : Molecular and metabolic basis and therapeutic outlook", Mol Genet Metab, 127, pp.12~22 (2019)[DOI]
- Prickett AR, "Imprinted gene expression and function of the dopa decarboxylase gene in the developing heart", Front Cell Dev Biol, 9, pp.676543~ (2021)[DOI]
- Fernnndez-Reina A, "What we know and what we need to know about aromatic and cationic biogenic amines in the gastrointestinal tract", Foods, 7, pp.145~ (2018)[DOI]
- Dagong MIA, "Carcass and physical meat characteristics of thin tail sheep(TTS)based on calpastatin gene(CAST)(Locus intron 5–exon 6)genotypes variation", Indonesian J Anim Vet Sci, 17, pp.13~24 (2012)[DOI]
- Latimer GW, "Official methods of analysis of AOAC international" (2012), AOAC International
- Cinar MU, "Association and expression quantitative trait loci(eQTL)analysis of porcine AMBP, GC and PPP1R3B genes with meat quality traits", Mol Biol Rep, 39, pp.4809~4821 (2012)[DOI]
- Rao X, "An improvement of the 2ˆ(-delta delta CT)method for quantitative real-time polymerase chain reaction data analysis", Biostat Bioinforma Biomath, 3, pp.71~85 (2013)
- Lachance J., "Encyclopedia of evolutionary", pp.208~211 (2016), Academic Press
- Yalcintan H, "Carcass and meat quality characteristics of lambs reared in different seasons", Arch Anim Breed, 60, pp.225~233 (2017)[DOI]
- Purbowati E, "Productivity and carcass characteristics of lambs fed fibrous agricultural wastes to substitute grass", Vet World, 14, pp.1559~1563 (2021)[DOI]
- Dicky Tri Utama, "Effects of cooking method and final core-temperature on cooking loss, lipid oxidation, nucleotide-related compounds and aroma volatiles of Hanwoo brisket", Animal Bioscience, 31(2), pp.293~300 (2018), 아세아·태평양축산학회[DOI]
- Kim TW, "pH as analytical indicator for managing pork meat quality", Sains Malaysiana, 45, pp.1097~1103 (2016)[DOI]
- Wang B, "Carcass traits, meat quality, and volatile compounds of lamb meat from different restricted grazing time and indoor supplementary feeding systems", Foods, 10, pp.2822~ (2021)[DOI]
- Polidori P, "The effects of slaughter age on carcass and meat quality of Fabrianese lambs", Small Rumin Res, 155, pp.12~15 (2017)[DOI]
- Hasanah U, "Carcass composition of fat tailed sheep at different slaughter weight", IOP Conf Ser Earth Environ Sci, 260, pp.012055~ (2019)[DOI]
- Majdoub-Mathlouthi L, "Effect of concentrate level and slaughter body weight on growth performances, carcass traits and meat quality of Barbarine lambs fed oat hay based diet", Meat Sci, 93, pp.557~563 (2013)[DOI]
- Furqon, "Expression and association of SCD gene polymorphisms and fatty acid compositions in chicken cross", Media Peternakan, 40, pp.151~157 (2017)[DOI]
- Gunawan A, "Identification of DGAT1 gene polymorphisms and its association with carcass characteristics and fatty acid traits in sheep", JITRO, 6, pp.267~274 (2019)
- Harahap RS, "The polymorphism and expression of CYP2E1 gene and its relation to carcass and meat quality of Indonesian lamb", Trop Anim Sci J, 44, pp.377~385 (2021)[DOI]
- Senyilmaz-Tiebe D, "Dietary stearic acid regulates mitochondria in vivo in humans", Nat Commun, 9, pp.3129~ (2018)[DOI]
- Sahadevan S, "Pathway based analysis of genes and interactions influencing porcine testis samples from boars with divergent androstenone content in back fat", PLoS One, 9, pp.e91077~ (2014)[DOI]
- Chen J, "Nutritional indices for assessing fatty acids : a mini-review", Int J Mol Sci, 21, pp.5695~ (2020)[DOI]
- Weaver CM, "Potassium and health", Adv Nutr, 4, pp.368~ (2013)[DOI]
- "Potassium" (2022)[URL]
- Frakolaki E, "Emerging role of l-dopa decarboxylase in flaviviridae virus infections", Cells, 8, pp.837~ (2019)[DOI]
