Association of single-nucleotide polymorphisms in dual specificity phosphatase 8 and insulin-like growth factor 2 genes with inosine-5′-monophosphate, inosine, and hypoxanthine contents in chickens
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Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line). Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of DUSP8 and IGF2 genes, respectively. One SNP (rs313443014 C>T) in DUSP8 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens. Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The IGF2 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed. Conclusion: The SNPs in the DUSP8 and IGF2 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.
Abstract
Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line). Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of DUSP8 and IGF2 genes, respectively. One SNP (rs313443014 C>T) in DUSP8 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens. Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The IGF2 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed. Conclusion: The SNPs in the DUSP8 and IGF2 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.
참고문헌 (55)
- Kubberød E, "Gender specific preferences and attitudes towards meat", Food Qual Prefer, 13, pp.285~294 (2002)[DOI]
- Elzerman JE, "Consumer acceptance and appropriateness of meat substitutes in a meal context", Food Qual Prefer, 22, pp.233~240 (2011)[DOI]
- Benningstad NCG, "Dissociating meat from its animal origins : A systematic literature review", Appetite, 147, pp.104554~ (2020)[DOI]
- Wanwisa Chumngoen, "Relationships between Descriptive Sensory Attributes and Physicochemical Analysis of Broiler and Taiwan Native Chicken Breast Meat", Animal Bioscience, 28(7), pp.1028~1037 (2015), 아세아·태평양축산학회[DOI]
- Marangoni F, "Role of poultry meat in a balanced diet aimed at maintaining health and wellbeing : An Italian consensus document", Food Nutr Res, 59, pp.27606~ (2015)[DOI]
- Jean Pierre Munyaneza, "Genome-wide association studies of meat quality traits in chickens: a review", Korean Journal of Agricultural Science, 49(3), pp.407~420 (2022), 농업과학연구소[DOI]
- Shil Jin, "Identification of quantitative trait loci for the fatty acid composition in Korean native chicken", Animal Bioscience, 31(8), pp.1134~1140 (2018), 아세아·태평양축산학회[DOI]
- Seo D, "Association of SNPs in AMY1A and AMY2A genes with chicken meat quality and clinicalchemical traits in chicken", J Fac Agric Kyushu Univ, 61, pp.121~125 (2016)[DOI]
- 최준호, "Differences in the Quality Characteristics between Commercial Korean Native Chickens and Broilers", 한국축산식품학회지, 30(1), pp.13~19 (2010), 한국축산식품학회
- Cahyadi Muhammad, "Association of the thyroid hormone responsive spot 14 alpha gene with growth-related traits in Korean native chicken", Animal Bioscience, 33(11), pp.1755~1762 (2020), 아세아·태평양축산학회[DOI]
- Jung S, "Carnosine, anserine, creatine, and inosine-5′-monophosphate contents in breast and thigh meats from 5 lines of Korean native chicken", Poult Sci, 92, pp.3275~3282 (2013)[DOI]
- Seo DW, "QTL analyses of general compound, color, and pH traits in breast and thigh muscles in Korean native chicken", Livest Sci, 182, pp.145~150 (2015)[DOI]
- S. Jin, "A Major Locus for Quantitatively Measured Shank Skin Color Traits in Korean Native Chicken", Animal Bioscience, 29(11), pp.1555~1561 (2016), 아세아·태평양축산학회[DOI]
- Jin S, "The breeding history and commercial development of the Korean native chicken", Worlds Poult Sci J, 73, pp.163~174 (2017)[DOI]
- Cha J, "Genome-wide association study identifies 12 loci associated with body weight at age 8weeks in Korean native chickens", Genes(Basel), 12, pp.1170~ (2021)[DOI]
- 정연국, "Comparison of Quality Traits of Thigh Meat from Korean Native Chickens and Broilers", 한국축산식품학회지, 31(5), pp.684~692 (2011), 한국축산식품학회
- Uemoto Y, "Effect of two nonsynonymous ecto-5′-nucleotidase variants on the genetic architecture of inosine 5′-monophosphate(IMP)and its degradation products in Japanese Black beef", BMC Genomics, 18, pp.874~ (2017)[DOI]
- Jayasena DD, "Factors affecting cooked chicken meat flavour : a review", World’s Poult Sci J, 69, pp.515~526 (2013)[DOI]
- Garmyn A, "Consumer preferences and acceptance of meat products", Foods, 9, pp.708~ (2020)[DOI]
- Mir NA, "Determinants of broiler chicken meat quality and factors affecting them : a review", J Food Sci Technol, 54, pp.2997~3009 (2017)[DOI]
- Khan MI, "Meat flavor precursors and factors influencing flavor precursors-A systematic review", Meat Sci, 110, pp.278~284 (2015)[DOI]
- Jayasena DD, "Comparison of the amounts of taste-related compounds in raw and cooked meats from broilers and Korean native chickens", Poult Sci, 93, pp.3163~3170 (2014)[DOI]
- Ishamri Ismail, "Poultry Meat Quality in Relation to Muscle Growth and Muscle Fiber Characteristics", 한국축산식품학회지, 37(6), pp.873~883 (2017), 한국축산식품학회
- Phatthawin Lengkidworraphiphat, "Taste-Active and Nutritional Components of Thai Native Chicken Meat: A Perspective of Consumer Satisfaction", 한국축산식품학회지, 41(2), pp.237~246 (2021), 한국축산식품학회[DOI]
- Rikimaru K, "Evaluation of the meat from Hinai-jidori chickens and broilers : Analysis of general biochemical components, free amino acids, inosine 5′-monophosphate, and fatty acids", J Appl Poult Res, 19, pp.327~333 (2010)[DOI]
- G. H. Chen, "Factors Affecting the Inosine Monophosphate Content of Muscles in Taihe Silkies Chickens", Animal Bioscience, 15(9), pp.1359~1363 (2002), 아세아·태평양축산학회
- Van der Steen, "Application of genomics to the pork industry", J Anim Sci, 83, pp.E1~E8 (2005)[DOI]
- Anggraeni A, "Identification of poymorphism and association analyses of FMO3 gene related with carcass and meat quality in Cihateup duck", Anim Prod, 19, pp.151~159 (2017)
- Geng X, "A genomewide association study reveals that genes with functions for bone development are associated with body conformation in catfish", Mar Biotechnol, 19, pp.570~578 (2017)[DOI]
- Zhao L, "A genome-wide association study identifies the genomic region associated with shell color in Yesso scallop, Patinopecten yessoensis", Mar Biotechnol, 19, pp.301~309 (2017)[DOI]
- Gunawan A, "Potential functional variants for fatness, carcass and meat quality traits in exon 3 of fat mass and obesity-associated gene in Indonesian ducks", Int J Poult Sci, 17, pp.443~451 (2018)[DOI]
- Chen HF, "Regulation of dual-specificity phosphatase(DUSP)ubiquitination and protein stability", Int J Mol Sci, 20, pp.2668~ (2019)[DOI]
- Ferguson BS, "Dual-specificity phosphatases regulate mitogen-activated protein kinase signaling in adipocytes in response to inflammatory stress", Cell Signal, 53, pp.234~245 (2019)[DOI]
- Hink R, "Investigation of DUSP8 and CALCA in alcohol dependence", Addict Biol, 8, pp.305~312 (2003)[DOI]
- Ding T, "DUSP8 phosphatase : Structure, functions, expression regulation and the role in human diseases", Cell Biosci, 9, pp.70~ (2019)[DOI]
- Cargnello M, "Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases", Microbiol Mol Biol Rev, 75, pp.50~83 (2011)[DOI]
- Dae Gwin Jeong, "The family-wide structure and function of human dual-specificity protein phosphatases", Acta Crystallographica Section D Biological Crystallography, 70(2), pp.421~435 (2014), International Union of Crystallography (IUCr)[DOI]
- Schriever SC, "Type 2 diabetes risk gene Dusp8 regulates hypothalamic Jnk signaling and insulin sensitivity", J Clin Investig, 130, pp.6093~6108 (2020)[DOI]
- Amills M, "Identification of three single nucleotide polymorphisms in the chicken insulinlike growth factor 1 and 2 genes and their associations with growth and feeding traits", Poult Sci, 82, pp.1485~1493 (2003)[DOI]
- Wang G, "Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken", Sci China Life Sci, 48, pp.187~194 (2005)[DOI]
- Yan L, "Exploring the genetic variants of insulin-like growth factor II gene and their associations with two production traits in Langshan chicken", J Appl Anim Res, 45, pp.60~63 (2017)[DOI]
- Ye J, "Mechanism of insulin resistance in obesity : a role of ATP", Front Med, 15, pp.372~382 (2021)[DOI]
- Criado-Mesas L, "Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition", PLoS ONE, 14, pp.e0220708~ (2019)[DOI]
- McTaggart JS, "The role of the KATP channel in glucose homeostasis in health and disease : More than meets the islet", J Physiol, 588, pp.3201~3209 (2010)[DOI]
- Bonora M, "ATP synthesis and storage", Purinergic Signal, 8, pp.343~357 (2012)[DOI]
- Oprea E, "sports and energy drinks", pp.65~129 (2019), Woodhead publishing
- Kim HC, "Potential of 2D qNMR spectroscopy for distinguishing chicken breeds based on the metabolic differences", Food Chem, 342, pp.128316~ (2021)[DOI]
- Nei M, "Molecular evolution and phylogenetics" (2000), Oxford University Press
- Hartl DL, "Principle of population genetics" (1997), Sinauer Associates
- "R: A language and environment for statistical computing" (2022)[URL]
- Hall TA, "BioEdit : An important software for molecular biology", GERF Bull Biosci, 2, pp.60~61 (2011)
- Hu J, "Genetic polymorphisms of the AMPD1 gene and their correlations with IMP contents in Fast Partridge and Lingshan chickens", Gene, 574, pp.204~209 (2015)[DOI]
- Prabuddha Manjula, "POU class 1 homeobox 1 gene polymorphisms associated with growth traits in Korean native chicken", Animal Bioscience, 31(5), pp.643~649 (2018), 아세아·태평양축산학회[DOI]
- Allendorf FW, "Conservation and genetics of populations", pp.118~132 (2013), John Wiley and Sons
- Munyaneza JP, "Exploring effects of betaine-homocysteine methyltransferase(BHMT)gene polymorphisms on fatty acid traits and cholesterol in sheep", J Indonesian Trop Anim Agric, 44, pp.243~251 (2019)[DOI]
