Phylogenetic and expression analysis of the angiopoietin-like gene family and their role in lipid metabolism in pigs
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Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.
Abstract
Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.
참고문헌 (45)
- Abu-Farha M, "The multi-faces of Angptl8 in health and disease : Novel functions beyond lipoprotein lipase modulation", Prog Lipid Res, 80, pp.101067~ (2020)[DOI]
- Zhang R, "Emerging roles of Lipasin as a critical lipid regulator", Biochem Biophys Res Commun, 432, pp.401~405 (2013)[DOI]
- Morelli MB, "Angiopoietin-like proteins as therapeutic targets for cardiovascular disease : focus on lipid disorders", Expert Opin Ther Targets, 24, pp.79~88 (2020)[DOI]
- Aron-Wisnewsky J, "Metabolism and metabolic disorders and the microbiome : the intestinal microbiota associated with obesity, lipid metabolism, and metabolic health-pathophysiology and therapeutic strategies", Gastroenterology, 160, pp.573~599 (2021)[DOI]
- Li J, "Triglyceride metabolism and angiopoietin-like proteins in lipoprotein lipase regulation", Clin Chim Acta, 503, pp.19~34 (2020)[DOI]
- Davies BS, "GPIHBP1 is responsible for the entry of lipoprotein lipase into capillaries", Cell Metab, 12, pp.42~52 (2010)[DOI]
- Mohamed F, "ANGPTL3 as a drug target in hyperlipidemia and atherosclerosis", Curr Atheroscler Rep, 24, pp.959~967 (2022)[DOI]
- Kersten S, "Angiopoietin-like 3 in lipoprotein metabolism", Nat Rev Endocrinol, 13, pp.731~739 (2017)[DOI]
- Wang Y, "Hepatic ANGPTL3regulates adipose tissue energy homeostasis", Proc Natl Acad Sci USA, 112, pp.11630~11635 (2015)[DOI]
- Ruppert PMM, "Fasting induces ANGPTL4 and reduces LPL activity in human adipose tissue", Mol Metab, 40, pp.101033~ (2020)[DOI]
- Bäckhed F, "The gut microbiota as an environmental factor that regulates fat storage", Proc Natl Acad Sci USA, 101, pp.15718~15723 (2004)[DOI]
- Alex S, "ANGPTL4 is produced by entero-endocrine cells in the human intestinal tract", Histochem Cell Biol, 141, pp.383~391 (2014)[DOI]
- Ruscica M, "Pharmacological aspects of ANGPTL3 and ANGPTL4 inhibitors : New therapeutic approaches for the treatment of atherogenic dyslipidemia", Pharmacol Res, 153, pp.104653~ (2020)[DOI]
- Mattijssen F, "Angptl4 serves as an endogenous inhibitor of intestinal lipid digestion", Mol Metab, 3, pp.135~144 (2014)[DOI]
- Dijk W, "Regulation of lipid metabolism by angiopoietin-like proteins", Curr Opin Lipidol, 27, pp.249~256 (2016)[DOI]
- Chen YQ, "Angiopoietin-like protein 4(E40K)and ANGPTL4", 8 complex have reduced, temperature-dependent LPL-inhibitory activity compared to ANGPTL4, 2021, pp.534, 498~503
- Zhang R, "Lipasin, a novel nutritionally-regulated liver-enriched factor that regulates serum triglyceride levels", Biochem Biophys Res Commun, 424, pp.786~792 (2012)[DOI]
- Chi X, "ANGPTL8 promotes the ability of ANGPTL3 to bind and inhibit lipoprotein lipase", Mol Metab, 6, pp.1137~1149 (2017)[DOI]
- Quagliarini F, "Atypical angiopoietin-like protein that regulates ANGPTL3", Proc Natl Acad Sci USA, 109, pp.19751~19756 (2012)[DOI]
- Zhang R, "The ANGPTL3-4-8 model, a molecular mechanism for triglyceride trafficking", Open Biol, 6, pp.150272~ (2016)[DOI]
- Liu YZ, "Angiopoietin-like proteins in atherosclerosis", Clin Chim Acta, 521, pp.19~24 (2021)[DOI]
- Poledne R, "Experimental models of hyperlipoproteinemia and atherosclerosis", Physiol Res, 66(Suppl 1), pp.S69~s75 (2017)[DOI]
- Xiao Y, "Comparative biogeography of the gut microbiome between Jinhua and Landrace pigs", Sci Rep, 8, pp.5985~ (2018)[DOI]
- Geer LY, "The NCBI BioSystems database", Nucleic Acids Res, 38(Suppl 1), pp.D492~ (2010)[DOI]
- Blanton LS, "Differentiation of rickettsia felis and rickettsia felis-like organisms via restriction fragment length polymorphism analysis", Vector Borne Zoonotic Dis, 19, pp.637~639 (2019)[DOI]
- Thompson JD, "CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22, pp.4673~4680 (1994)[DOI]
- Guan Z, "Identification and expression analysis of bovine ANGPTL gene family", Curr Zool, 56, pp.445~453 (2010)[DOI]
- Kumar S, "MEGA7 : Molecular evolutionary genetics analysis version 7. 0 for bigger datasets", Mol Biol Evol, 33, pp.1870~1874 (2016)[DOI]
- Louis A, "Genomicus update 2015 : KaryoView and MatrixView provide a genomewide perspective to multispecies comparative genomics", Nucleic Acids Res, 43, pp.D682~ (2015)[DOI]
- Yang H, "Uncovering the composition of microbial community structure and metagenomics among three gut locations in pigs with distinct fatness", Sci Rep, 6, pp.27427~ (2016)[DOI]
- Jin L, "Modulation of gene expression in liver of hibernating asiatic toads(Bufo gargarizans)", Int J Mol Sci, 19, pp.2363~ (2018)[DOI]
- Yan H, "Shortchain fructo-oligosaccharides alleviates oxidized oil-induced intestinal dysfunction in piglets associated with the modulation of gut microbiota", J Funct Foods, 64, pp.103661~ (2020)[DOI]
- Yang H, "Gut microbiota is a major contributor to adiposity in pigs", Front Microbiol, 9, pp.3045~ (2018)[DOI]
- Hua Yang, "Core gut microbiota in Jinhua pigs and its correlation with strain, farm and weaning age", The Journal of Microbiology, 56(5), pp.346~355 (2018), 한국미생물학회[DOI]
- Ono M, "Protein region important for regulation of lipid metabolism in angiopoietinlike 3(ANGPTL3) : ANGPTL3 is cleaved and activated in vivo", J Biol Chem, 278, pp.41804~41809 (2003)[DOI]
- Feng SQ, "Cloning, chromosome mapping and expression characteristics of porcine ANGPTL3 and-4", Cytogenet Genome Res, 114, pp.44~49 (2006)[DOI]
- Costa RA, "Evolution of the angiopoietin-like gene family in teleosts and their role in skin regeneration", BMC Evol Biol, 17, pp.14~ (2017)[DOI]
- Dehal P, "Two rounds of whole genome duplication in the ancestral vertebrate", PLoS Biol, 3, pp.e314~ (2005)[DOI]
- Kristensen KK, "Unfolding of monomeric lipoprotein lipase by ANGPTL4 : Insight into the regulation of plasma triglyceride metabolism", Proc Natl Acad Sci USA, 117, pp.4337~4346 (2020)[DOI]
- Catalano-Iniesta L, "Evidences for Expression and location of ANGPTL8in human adipose tissue", J Clin Med, 9, pp.512~ (2020)[DOI]
- Santulli G, "Angiopoietin-like proteins : a comprehensive look", Front Endocrinol(Lausanne), 5, pp.4~ (2014)[DOI]
- Barja-Fernández S, "ANGPTL-4is associated with obesity and lipid profile in children and adolescents", Nutrients, 11, pp.1340~ (2019)[DOI]
- Xiao HB, "ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice", Exp Mol Pathol, 103, pp.242~248 (2017)[DOI]
- Chi X, "ANGPTL8 promotes the ability of ANGPTL3 to bind and inhibit lipoprotein lipase", Mol Metab, 6, pp.1137~1149 (2017)[DOI]
- Miao ZG, "Developmental changes of carcass composition, meat quality and organs in the Jinhua pig and Landrace", Animal, 3, pp.468~473 (2009)[DOI]