MiR-188-5p regulates the proliferation and differentiation of goat skeletal muscle satellite cells by targeting calcium/calmodulin dependent protein kinase II beta
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: The aim of this study was to reveal the role and regulatory mechanism of miR- 188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.
Abstract
Objective: The aim of this study was to reveal the role and regulatory mechanism of miR- 188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.
참고문헌 (37)
- Wang Y, "Long noncoding RNA H19mediates LCoR to impact the osteogenic and adipogenic differentiation of mBMSCs in mice through sponging miR-188", J Cell Physiol, 233, pp.7435~7446 (2018)[DOI]
- Lyu M, "chi-miR-487b-3p inhibits goat myoblast proliferation and differentiation by targeting IRS1 through the IRS1", PI3K, Int J Mol Sci, pp.2021, 23[DOI]
- Zammit PS, "Function of the myogenic regulatory factors Myf5, MyoD, Myogenin and MRF4 in skeletal muscle, satellite cells and regenerative myogenesis", Semin Cell Dev Biol, 72, pp.19~32 (2017)[DOI]
- Tierney MT, "Satellite cell heterogeneity in skeletal muscle homeostasis", Trends Cell Biol, 26, pp.434~444 (2016)[DOI]
- Hindi SM, "All for one and one for all : regenerating skeletal muscle", Cold Spring Harb Perspect Biol, 14, pp.a040824~ (2022)[DOI]
- Giuliani G, "Signaling pathways regulating the fate of fibro", adipogenic progenitors(FAPs)in skeletal muscle regeneration and disease, 2022, pp.289, 6484~6517
- Ling YH, "miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat", Sci Rep, 8, pp.3909~ (2018)[DOI]
- Punch VG, "Transcriptional networks that regulate muscle stem cell function", Wiley Interdiscip Rev Syst Biol Med, 1, pp.128~140 (2009)[DOI]
- Lu TX, "MicroRNA", J Allergy Clin Immunol, 141, pp.1202~1207 (2018)[DOI]
- Bjorkman KK, "miR-206 enforces a slow muscle phenotype", J Cell Sci, 133 (2020)[DOI]
- Chen JF, "The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation", Nat Genet, 38, pp.228~233 (2006)[DOI]
- Cardinali B, "MicroRNA-222regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells", Cell Death Dis, 7, pp.e2086~ (2016)[DOI]
- Ling Y, "RNA-Seq reveals miRNA role shifts in seven stages of skeletal muscles in goat fetuses and kids", Front Genet, 11, pp.684~ (2020)[DOI]
- Shibasaki H, "Characterization of a novel microRNA, miR-188, elevated in serum of muscular dystrophy dog model", PLoS One, 14, pp.e0211597~ (2019)[DOI]
- Mi S, "miR-188-3p inhibits vascular smooth muscle cell proliferation and migration by targeting fibroblast growth factor 1(FGF1)", Med Sci Monit, 26, pp.e924394~ (2020)[DOI]
- Ying-hui L, "Switches in transcriptome functions during seven skeletal muscle development stages from fetus to kid in Capra hircus", J Integr Agric, 20, pp.212~226 (2021)[DOI]
- Kim DY, "Regulatory role of microRNAs in the proliferation and differentiation of adipose-derived stem cells", Histol Histopathol, 32, pp.1~10 (2017)[DOI]
- Ran X, "Regulation of embryonic stem cell self-renewal and differentiation by MicroRNAs", Cell Reprogram, 19, pp.150~158 (2017)[DOI]
- Zhao Y, "Effects of miRNAs on myocardial apoptosis by modulating mitochondria related proteins", Clin Exp Pharmacol Physiol, 44, pp.431~440 (2017)[DOI]
- Hu Z, "miRNAs in synapse development and synaptic plasticity", Curr Opin Neurobiol, 45, pp.24~31 (2017)[DOI]
- Bhattacharya M, "The crucial role and regulations of miRNAs in zebrafish development", Protoplasma, 254, pp.17~31 (2017)[DOI]
- Baradaran B, "Dysregulation of key microRNAs in pancreatic cancer development", Biomed Pharmacother, 109, pp.1008~1015 (2019)[DOI]
- Zhu X, "MicroRNA-188-5p promotes apoptosis and inhibits cell proliferation of breast cancer cells via the MAPK signaling pathway by targeting Rap2c", J Cell Physiol, 235, pp.2389~2402 (2020)[DOI]
- Ma J, "LncRNA PAPAS promotes hepatocellular carcinoma by interacting with miR-188-5p", J Cell Biochem, 120, pp.13494~13500 (2019)[DOI]
- Riaz F, "Inhibition of miR-188-5p alleviates hepatic fibrosis by significantly reducing the activation and proliferation of HSCs through PTEN", PI3K, J Cell Mol Med, pp.2021, 25[DOI]
- Zhu X, "miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cells", Oncol Lett, 21, pp.481~ (2021)[DOI]
- Zhang Q, "MicroRNAs as big regulators of neural stem", progenitor cell proliferation, differentiation and migration : a potential treatment for stroke, 2017, pp.23, 2252~2257
- Fang F, "MicroRNA-188-5p suppresses tumor cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma", J Hepatol, 63, pp.874~885 (2015)[DOI]
- Valenti MT, "Physical exercise modulates miR-21-5p, miR-129-5p, miR-378-5p, and miR-188-5p expression in progenitor cells promoting osteogenesis", Cells, 8, pp.742~ (2019)[DOI]
- Küry S, "De novo mutations in protein kinase genes CAMK2A and CAMK2B cause intellectual disability", Am J Hum Genet, 101, pp.768~788 (2017)[DOI]
- Nicole O, "CaMKIIβ in Neuronal development and plasticity : an emerging candidate in brain diseases", Int J Mol Sci, 21, pp.7272~ (2020)[DOI]
- Akita T, "De novo variants in CAMK2A and CAMK2B cause neurodevelopmental disorders", Ann Clin Transl Neurol, 5, pp.280~296 (2018)[DOI]
- Franz A, "Branch point strength controls species-specific CAMK2B alternative splicing and regulates LTP", Life Sci Alliance, 6, pp.e202201826~ (2023)[DOI]
- Dwyer BK, "Case report : developmental delay and acute neuropsychiatric episodes associated with a de novo mutation in the CAMK2B gene(c. 328G>A p. Glu110Lys)", Front Pharmacol, 13, pp.794008~ (2022)[DOI]
- Kool MJ, "The molecular, temporal and region-specific requirements of the beta isoform of Calcium", Calmodulin-dependent protein kinase type 2(CAMK2B)in mouse locomotion, 2016, pp.6, 26989~
- Yuasa K, "Targeted ablation of p38αMAPK suppresses denervation-induced muscle atrophy", Sci Rep, 8, pp.9037~ (2018)[DOI]
- Brentnall M, "Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis", BMC Mol Cell Biol, 14, pp.32~ (2013)[DOI]
