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Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows

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Objective: Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay.Methods: A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics.Results: The results showed that <i>Ralstonia</i> and <i>Sphingomonas</i> were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes.Conclusion: These results reveal the relative abundance of <i>Ralstonia</i> and <i>Sphingomonas</i> other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.

Abstract

Objective: Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods: A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results: The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion: These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.

참고문헌 (27)

  1. Oikonomou G, "Microbiota of cow's milk; distinguishing healthy, sub-clinically and clinically diseased quarters", PLoS One, 9, pp.e85904~ (2014)[DOI]
  2. Derakhshani H, "Invited review : Microbiota of the bovine udder : Contributing factors and potential implications for udder health and mastitis susceptibility", J Dairy Sci, 101, pp.10605~10625 (2018)[DOI]
  3. Addis MF, "The bovine milk microbiota : insights and perspectives from-omics studies", Mol Biosyst, 12, pp.2359~2372 (2016)[DOI]
  4. Kuehn JS, "Bacterial community profiling of milk samples as a means to understand culturenegative bovine clinical mastitis", Plos One, 8, pp.e61959~ (2013)[DOI]
  5. Pang MD, "Insights into the bovine milk microbiota in dairy farms with different incidence rates of subclinical mastitis", Front Microbiol, 9, pp.2379~ (2018)[DOI]
  6. Wang Y, "Coupling 16S rDNA sequencing and untargeted mass spectrometry for milk microbial composition and metabolites from dairy cows with clinical and subclinical mastitis", J Agric Food Chem, 68, pp.8496~8508 (2020)[DOI]
  7. Song MY, "Combined analysis of DNA methylome and transcriptome reveal novel candidate genes with susceptibility to bovine Staphylococcus aureus subclinical mastitis", Sci Rep-Uk, 6, pp.29390~ (2016)[DOI]
  8. Dahlberg J, "Microbiota of bovine milk, teat skin, and teat canal : Similarity and variation due to sampling technique and milk fraction", J Dairy Sci, 103, pp.7322~7330 (2020)[DOI]
  9. Patel SH, "Culture independent assessment of human milk microbial community in lactational mastitis", Sci Rep-Uk, 7, pp.7804~ (2017)[DOI]
  10. Soto Del Rio MLD, "Characterization of bacterial communities of donkey milk by high-throughput sequencing", Int J Food Microbiol, 251, pp.67~72 (2017)[DOI]
  11. Papademas P, "Investigation of donkey milk bacterial diversity by 16S rDNA high-throughput sequencing on a Cyprus donkey farm", J Dairy Sci, 104, pp.167~178 (2021)[DOI]
  12. Ryan MP, "Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. Diversity in Ralstonia pickettii", BMC Microbiol, 11, pp.194~ (2011)[DOI]
  13. Nam HM, "Prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in Korea", J Dairy Sci, 92, pp.2020~2026 (2009)[DOI]
  14. Richard ML, "Mucosa-associated microbiota dysbiosis in colitis associated cancer", Gut Microbes, 9, pp.131~142 (2018)[DOI]
  15. Green HD, "Ralstonia infection in cystic fibrosis", Epidemiol Infect, 145, pp.2864~2872 (2017)[DOI]
  16. Sung JJY, "Gastric microbes associated with gastric inflammation, atrophy and intestinal metaplasia 1 year after Helicobacter pylori eradication", Gut, 69, pp.1572~1581 (2020)[DOI]
  17. Chong LYZ, "Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors : novel markers in biological classification", Breast Cancer Res Treat, 132, pp.143~151 (2012)[DOI]
  18. Lee YY, "Overexpression of transcobalamin 1 is an independent negative prognosticator in rectal cancers receiving concurrent chemoradiotherapy", J Cancer, 8, pp.1330~1337 (2017)[DOI]
  19. Guan YY, "Uridine phosphorylase 1 associates to biological and clinical significance in thyroid carcinoma cell lines", J Cell Mol Med, 23, pp.7438~7448 (2019)[DOI]
  20. Maldonado RF, "Lipopolysaccharide modification in Gram-negative bacteria during chronic infection", FEMS Microbiol Rev, 40, pp.480~493 (2016)[DOI]
  21. Yadav RK, "Alpha-linolenic acid based nano-suspension protect against lipopolysaccharides induced mastitis by inhibiting NFkappaBp65, HIF-1alpha, and mitochondria-mediated apoptotic pathway in albino Wistar rats", Toxicol Appl Pharmacol, 377, pp.114628~ (2019)[DOI]
  22. Fernandez MF, "Breast cancer and its relationship with the microbiota", Int J Environ Res Public Health, 15, pp.1747~ (2018)[DOI]
  23. Rault L, "Bovine teat cistern microbiota composition and richness are associated with the immune and microbial responses during transition to once-daily milking", Front Microbiol, 11, pp.602404~ (2020)[DOI]
  24. Basu M, "Predicting tissuespecific gene expression from whole blood transcriptome", Sci Adv, 7, pp.eabd6991~ (2021)[DOI]
  25. Schwarz D, "Microscopic differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands", J Dairy Res, 78, pp.448~455 (2011)[DOI]
  26. Falentin H, "Bovine teat microbiome analysis revealed reduced alpha diversity and significant changes in taxonomic profiles in quarters with a history of mastitis", Front Microbiol, 7, pp.480~ (2016)[DOI]
  27. Metzger SA, "A cohort study of the milk microbiota of healthy and inflamed bovine mammary glands from dryoff through 150 days in milk", Front Vet Sci, 5, pp.247~ (2018)[DOI]

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