Higher concentrations of folic acid reduced the dietary requirements of supplemental methionine for commercial broilers
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: An experiment was conducted to study the effect of supplementing DL methionine (DL Met) at graded concentrations on performance, carcass variables, immune responses and antioxidant variables in broiler chicken fed folic acid (FA) fortified (4 mg/kg) lowmethionine diet.Methods: A basal diet (BD) without supplemental DL Met, but with higher level (4 mg/kg) of FA and a control diet (CD) with the recommended concentration of methionine (Met) were prepared. The BD was supplemented with DL Met at graded concentrations (0%, 10%, 20%, 30%, 40%, and 50% supplemental DL Met of CD). Each diet was fed ad libitum to 10 replicates of 5 broiler male chicks in each from 1 to 42 d of age.Results: Body weight gain (BWG) reduced, and feed conversion ratio (FCR) increased in broilers fed low-Met BD. At 30% and 20% inclusion of DL met, the BWG and FCR, respectively were similar to those fed the CD. Similarly, supplementation of 10% DL Met to the BD significantly increased ready to cook meat yield and breast meat weight, which were similar to those of the CD fed broilers. Lipid peroxidation reduced, the activity of antioxidant enzymes (GSHPx and GSHRx) in serum increased and lymphocyte proliferation increased with increased supplemental DL Met level in the BD. The concentrations of total protein and albumin in serum increased with DL Met supplementation to the BD.Conclusion: Based on the data, it can be concluded that supplemental Met can be reduced to less than 50% in broiler chicken diets (4.40, 3.94, and 3.39 g/kg, respectively in pre-starter, starter and finisher phases) containing 4 mg/kg FA.
Abstract
Objective: An experiment was conducted to study the effect of supplementing DL methionine (DL Met) at graded concentrations on performance, carcass variables, immune responses and antioxidant variables in broiler chicken fed folic acid (FA) fortified (4 mg/kg) lowmethionine diet. Methods: A basal diet (BD) without supplemental DL Met, but with higher level (4 mg/kg) of FA and a control diet (CD) with the recommended concentration of methionine (Met) were prepared. The BD was supplemented with DL Met at graded concentrations (0%, 10%, 20%, 30%, 40%, and 50% supplemental DL Met of CD). Each diet was fed ad libitum to 10 replicates of 5 broiler male chicks in each from 1 to 42 d of age. Results: Body weight gain (BWG) reduced, and feed conversion ratio (FCR) increased in broilers fed low-Met BD. At 30% and 20% inclusion of DL met, the BWG and FCR, respectively were similar to those fed the CD. Similarly, supplementation of 10% DL Met to the BD significantly increased ready to cook meat yield and breast meat weight, which were similar to those of the CD fed broilers. Lipid peroxidation reduced, the activity of antioxidant enzymes (GSHPx and GSHRx) in serum increased and lymphocyte proliferation increased with increased supplemental DL Met level in the BD. The concentrations of total protein and albumin in serum increased with DL Met supplementation to the BD. Conclusion: Based on the data, it can be concluded that supplemental Met can be reduced to less than 50% in broiler chicken diets (4.40, 3.94, and 3.39 g/kg, respectively in pre-starter, starter and finisher phases) containing 4 mg/kg FA.
참고문헌 (38)
- Takahashi K, "Influences of dietary methionine and cysteine on metabolic responses to immunological stress by Escherichia coli lipopolysaccharide injection, and mitogenic response in broiler chickens", Br J Nutr, 78, pp.815~821 (1997)[DOI]
- Rama Rao SV, "Interaction between genotype and dietary concentrations of methionine for immune function in commercial broilers", Br Poult Sci, 44, pp.104~112 (2003)[DOI]
- Wang ST, "Methionine and cysteine affect glutathione level, glutathione-related enzyme activities and the expression of glutathione s-transferase isozymes in rat hepatocytes", J Nutr, 127, pp.2135~2141 (1997)[DOI]
- Larbier M, "Nutrition and feeding of poultry" (1992), Nottingham University Press
- Pesti GM, "Factors influencing the assessment of the availability of choline in feedstuffs", Poult Sci, 60, pp.188~196 (1981)[DOI]
- Virtanen E, "Effects of betaine on methionine requirement of broilers under various environmental conditions", Proceedings, Australian Poultry Science Symposium, pp.88~92 (1995)
- Rama Rao SV, "Effect of supplementing betaine on performance, carcass traits and immune responses in broiler chicken fed diets containing different concentrations of methionine", Asian-Australas J Anim Sci, 24, pp.662~669 (2011)[DOI]
- Rama Rao SV, "Effect of methyl donors supplementation on performance, immune responses and anti-oxidant variables in broiler chicken fed diet without supplemental methionine", Anim Biosci, 35, pp.475~483 (2022)[DOI]
- Rama Rao SV, "Enhancement of performance and anti-oxidant variables in broiler chicken fed diets containing sub-optimal methionine level with graded concentrations of sulphur and folic acid", Anim Biosci, 35, pp.721~729 (2022)[DOI]
- Stover PJ, "Physiology of folate and vitamin B12 in health and disease", Nutr Rev, 62, pp.S3~S12 (2004)[DOI]
- Finkelstein JD, "Methionine metabolism in mammals. distribution of homocysteine between competing pathways", J Biol Chem, 259, pp.9508~9513 (1984)[DOI]
- Whitehead CC, "Folic acid requirements of broilers", Br Poult Sci, 36, pp.113~121 (1995)[DOI]
- Jing M, "Performance, serum biochemical responses, and gene expression of intestinal folate transporters of young and older laying hens in response to dietary folic acid supplementation and challenge with Escherichia coli lipopolysaccharide", Poult Sci, 93, pp.122~131 (2014)[DOI]
- Munyaka PM, "Immunomodulation in young laying hens by dietary folic acid and acute immune responses after challenge with Escherichia coli lipopolysaccharide", Poult Sci, 91, pp.2454~2463 (2012)[DOI]
- Munyaka PM, "Response of older laying hens to an Escherichia coli lipopolysaccharide challenge when fed diets with or without supplemental folic acid", Poult Sci, 92, pp.105~113 (2013)[DOI]
- Huang SR, "Folate depletion and elevated plasma homocysteine promote oxidative stress in rat livers", J Nutr, 131, pp.33~38 (2001)[DOI]
- Joshi R, "Free radical scavenging behavior of folic acid : evidence for possible antioxidant activity", Free Radic Biol Med, 30, pp.1390~1399 (2001)[DOI]
- Kshirsagar SS, "Development and validation of new uv-spectroscopic method for water soluble folic acid", Int J Chem Concepts, 3, pp.364~370 (2017)
- Llames CR, "Determination of amino acids in feeds : collaborative study", J AOAC Int, 77, pp.1362~1402 (1994)[DOI]
- Placer ZA, "Estimation of product of lipid peroxidation(malonyl dialdehyde)in biochemical systems", Anal Biochem, 16, pp.359~364 (1966)[DOI]
- Bergmeyer HU, "Methods of enzymatic analysis Vol 2", pp.165~166 (1983), Verlag Chemie
- Paglia DE, "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase", J Lab Clin Med, 70, pp.158~169 (1967)[DOI]
- Bounous DI, "Comparison of MTT colorimetric assay and tritiated thymidine uptake for lymphocyte proliferation assays using chicken splenocytes", Avian Dis, 36, pp.1022~1027 (1992)[DOI]
- Reynolds DL, "Protective immunity against Newcastle disease : the role of antibodies specific to Newcastle disease virus polypeptides", Avian Dis, 44, pp.138~144 (2000)[DOI]
- SAS Institute, "SAS User’s Guide. Statistics. Version 6.12", SAS Institute, ~ (1994)
- Harms RH, "Effects of supplemental methionine and potassium sulfate on the choline requirement of the turkey poult", Poult Sci, 63, pp.1464~1466 (1984)[DOI]
- Del Vesco AP, "Dietary methionine effects on IGF-I and GHR MRNA expression in broilers", Genetics Mol Res, 12, pp.6414~6423 (2013)[DOI]
- Martin WG, "Synthesis of taurine from sulfate by the chick", Proc Soc Exp Biol Med, 122, pp.841~844 (1966)[DOI]
- Pesti GM, "Folate deficiency in chicks fed diets containing practical ingredients", Poult Sci, 70, pp.600~604 (1991)[DOI]
- Akter N, "The impact of different levels of l-methionine(l-met)on carcass yield traits, serum metabolites, tibial characters, and profitability of broilers fed conventional diet", J Adv Vet Anim Res, 7, pp.253~259 (2020)[DOI]
- Acar N, "The effect of feeding excess methionine on live performance, carcass traits, and ascitic mortality", Poult Sci, 80, pp.1585~1589 (2001)[DOI]
- Miles RD, "The interrelationship between methionine, choline, and sulfate in broiler diets", Poult Sci, 62, pp.495~498 (1983)[DOI]
- Tillman PB, "The response of male broiler chicks to a corn-soy diet supplemented with L-Methionine, L-Cystine, Choline, Sulfate, and Vitamin B12", Poult Sci, 65, pp.1741~1748 (1986)[DOI]
- Tsiagbe VK, "Enhanced immune responses in broiler chicks fed methionine-supplemented diets", Poult Sci, 66, pp.1147~1154 (1987)[DOI]
- Maroufyan E, "Effect of dietary combination of methionine and fish oil on cellular immunity and plasma fatty acids in infectious bursal disease challenged chickens", Sci World J, 2013, pp.531397~ (2013)[DOI]
- Del Vesco AP, "Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers", Br J Nutr, 113, pp.549~559 (2015)[DOI]
- Ryu KS, "The folic acid requirements of starting broiler chicks fed diets based on practical ingredients.: 2. Interrelationships with dietary methionine", Poult Sci, 74, pp.1456~1462 (1995)[DOI]
- El-Demerdash FM, "Biochemical study on the protective role of folic acid in rabbits treated with chromium(VI)", J Env Sci Health B, 41, pp.731~746 (2006)[DOI]