Laying hen responses to multi-strain Bacillus-based probiotic supplementation from 25 to 37 weeks of age
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Objective: This study aimed to investigate the efficacy of Bacillus-based probiotics supplemented at two different levels to modulate the productive performance, egg quality, tibia traits, and specific cecal bacteria counts of Hy-Line Brown layers from 25 to 37 weeks of age.Methods: A total of 216 twenty-five-week-old hens were randomly distributed into 3 experimental diets with 12 replicates of 6 birds per cage. Diets included basal diet supplemented with 0 (CON), 3×108 (PRO1), or 3×109 (PRO2) colony-forming unit (CFU) of the test probiotic containing Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 per kilogram of feed.Results: Improved egg weights and mass at 29 weeks; and feed intake at 31 weeks (p<0.10) were noticed with the probiotic-supplemented PRO1 and PRO2 diets. Considering egg quality, the shell thickness, Haugh units, and yolk color were improved; but yolk cholesterol was lowered (p<0.05) with PRO1 and PRO2 diets at 29 weeks. At both 33 and 37 weeks, the egg-breaking strength, shell color and thickness, albumen height, Haugh units, and yolk color were improved; but yolk cholesterol was similarly lowered (p<0.05) with the PRO1 and PRO2 diets. Improved tibia Ca, ash, weights, and density; and raised cecal counts of Bifidobacteria and Lactobacilli (p<0.05) were noticed with PRO1 and PRO2 diets. Improved tibia P but reduced Clostridia counts (p<0.10) were also observed with the PRO1 and PRO2 diets.Conclusion: Probiotic supplementation of Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 at 3×108 CFU/kg of feed is adequate to significantly improve egg quality, lower yolk cholesterol, enhance several tibia traits, and raise the populations of beneficial cecal bacteria. Modest improvements in several productive parameters and tibia P but reduced Clostridia were also observed; and could warrant further investigation of probiotic effects beyond the current test period.
Abstract
Objective: This study aimed to investigate the efficacy of Bacillus-based probiotics supplemented at two different levels to modulate the productive performance, egg quality, tibia traits, and specific cecal bacteria counts of Hy-Line Brown layers from 25 to 37 weeks of age. Methods: A total of 216 twenty-five-week-old hens were randomly distributed into 3 experimental diets with 12 replicates of 6 birds per cage. Diets included basal diet supplemented with 0 (CON), 3×108 (PRO1), or 3×109 (PRO2) colony-forming unit (CFU) of the test probiotic containing Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 per kilogram of feed. Results: Improved egg weights and mass at 29 weeks; and feed intake at 31 weeks (p<0.10) were noticed with the probiotic-supplemented PRO1 and PRO2 diets. Considering egg quality, the shell thickness, Haugh units, and yolk color were improved; but yolk cholesterol was lowered (p<0.05) with PRO1 and PRO2 diets at 29 weeks. At both 33 and 37 weeks, the egg-breaking strength, shell color and thickness, albumen height, Haugh units, and yolk color were improved; but yolk cholesterol was similarly lowered (p<0.05) with the PRO1 and PRO2 diets. Improved tibia Ca, ash, weights, and density; and raised cecal counts of Bifidobacteria and Lactobacilli (p<0.05) were noticed with PRO1 and PRO2 diets. Improved tibia P but reduced Clostridia counts (p<0.10) were also observed with the PRO1 and PRO2 diets. Conclusion: Probiotic supplementation of Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 at 3×108 CFU/kg of feed is adequate to significantly improve egg quality, lower yolk cholesterol, enhance several tibia traits, and raise the populations of beneficial cecal bacteria. Modest improvements in several productive parameters and tibia P but reduced Clostridia were also observed; and could warrant further investigation of probiotic effects beyond the current test period.
참고문헌 (40)
- Sugiharto S, "Role of nutraceuticals in gut health and growth performance of poultry", J Saudi Soc Agric Sci, 15, pp.99~111 (2016)[DOI]
- Jha R, "Probiotics(direct-fed microbials)in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health : a systematic review", Animals(Basel), 10, pp.1863~ (2020)[DOI]
- Ramlucken U, "Advantages of Bacillus-based probiotics in poultry production", Livest Sci, 241, pp.104215~ (2020)[DOI]
- Gaggìa F, "Probiotics and prebiotics in animal feeding for safe food production", Int J Food Microbiol, 141, pp.S15~S28 (2010)[DOI]
- Yaqoob MU, "An updated review on probiotics as an alternative of antibiotics in poultry — A review", Anim Biosci, 35, pp.1109~1120 (2022)[DOI]
- Abd El-Hack ME, "Probiotics in poultry feed : a comprehensive review", J Anim Physiol Anim Nutr, 104, pp.1835~1850 (2020)[DOI]
- Panda AK, "Production performance, serum", yolk cholesterol and immune competence of white leghorn layers as influenced by dietary supplementation with probiotic, 2003, pp.35, 85~94
- Chowdhury SD, "Application of probiotics in layer diets : a review", Bangladesh J Anim Sci, 49, pp.1~12 (2020)[DOI]
- Park JH, "Egg production, egg quality, nutrient digestibility, and excreta microflora of laying hens fed with a diet containing brewer’s yeast hydrolysate", J Appl Anim Res, 48, pp.492~498 (2020)[DOI]
- Mutuş R, "The effect of dietary probiotic supplementation on tibial bone characteristics and strength in broilers", Poult Sci, 85, pp.1621~1625 (2006)[DOI]
- Abdelqader A, "Effects of dietary probiotic inclusion on performance, eggshell quality, cecal microflora composition, and tibia traits of laying hens in the late phase of production", Trop Anim Health Prod, 45, pp.1017~1024 (2013)[DOI]
- Mahdavi AH, "Effect of probiotic supplements on egg quality and laying hen's performance", Int J Poult Sci, 4, pp.488~492 (2005)[DOI]
- Mikulski D, "Effects of dietary probiotic(Pediococcus acidilactici)supplementation on performance, nutrient digestibility, egg traits, egg yolk cholesterol, and fatty acid profile in laying hens", Poult Sci, 91, pp.2691~2700 (2012)[DOI]
- Yalçın S, "Effects of dietary yeast autolysate(Saccharomyces cerevisiae)and black cumin seed(Nigella sativa L. )on performance, egg traits, some blood characteristics and antibody production of laying hens", Livest Sci, 145, pp.13~20 (2012)[DOI]
- Abdelqader A, "Effects of dietary Bacillus subtilis and inulin supplementation on performance, eggshell quality, intestinal morphology and microflora composition of laying hens in the late phase of production", Anim Feed Sci Technol, 179, pp.103~111 (2013)[DOI]
- Aziz Mousavi SMA, "A review of dietary probiotics in poultry", J Appl Biotechnol Rep, 5, pp.48~54 (2018)[DOI]
- Naidu KSB, "The use of probiotics and safety concerns : a review", Afr J Microbiol Res, 6, pp.6871~6877 (2012)[DOI]
- Oketch EO, "Physiology of lipid digestion and absorption in poultry : an updated review on the supplementation of exogenous emulsifiers in broiler diets", J Anim Physiol Anim Nutr, 107, pp.1429~1443 (2023)[DOI]
- Ribeiro V, "Effects of the dietary supplementation of Bacillus subtilis levels on performance, egg quality and excreta moisture of layers", Anim Feed Sci Technol, 195, pp.142~146 (2014)[DOI]
- "Hy-line brown commercial layers, management guide" (2018)[URL]
- Lei K, "Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens", Poult Sci, 92, pp.2389~2395 (2013)[DOI]
- Yörük MA, "The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens", Poult Sci, 83, pp.84~88 (2004)[DOI]
- Lin RL, "Genetic parameters of body weight, egg production, and shell quality traits in the Shan Ma laying duck(Anas platyrhynchos)", Poult Sci, 95, pp.2514~2519 (2016)[DOI]
- Li L, "Effects of a dried Bacillus subtilis culture on egg quality", Poult Sci, 85, pp.364~368 (2006)[DOI]
- Ishikawa Si, "Photodynamic antimicrobial activity of avian eggshell pigments", FEBS Lett, 584, pp.770~774 (2010)[DOI]
- Gautron J, "Review : production factors affecting the quality of chicken table eggs and egg products in Europe", Animal(Basel), 16, pp.100425~ (2022)[DOI]
- Choe DW, "Egg production, faecal pH and microbial population, small intestine morphology, and plasma and yolk cholesterol in laying hens given liquid metabolites produced by Lactobacillus plantarum strains", Br Poult Sci, 53, pp.106~115 (2012)[DOI]
- Zhang ZF, "Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens", J Anim Sci, 91, pp.4781~4787 (2013)[DOI]
- Souza O, "The impact of Bacillus subtilis PB6 and chromium propionate on the performance, egg quality and nutrient metabolizability of layer breeders", Animals(Basel), 11, pp.3084~ (2021)[DOI]
- Ahn YT, "Deconjugation of bile salts by Lactobacillus acidophilus isolates", Int Dairy J, 13, pp.303~311 (2003)[DOI]
- St-Onge MP, "Consumption of fermented and nonfermented dairy products : effects on cholesterol concentrations and metabolism", Am J Clin Nutr, 71, pp.674~681 (2000)[DOI]
- Odabaşi AZ, "Changes in brown eggshell color as the hen ages", Poult Sci, 86, pp.356~363 (2007)[DOI]
- Kim CH, "Age-related changes in egg quality of Hy-Line Brown hens", Int J Poult Sci, 13, pp.510~514 (2014)[DOI]
- Sirri F, "Variability and interaction of some egg physical and eggshell quality attributes during the entire laying hen cycle", Poult Sci, 97, pp.1818~1823 (2018)[DOI]
- Świątkiewicz S, "Dietary factors improving eggshell quality : an updated review with special emphasis on microelements and feed additives", Worlds Poult Sci J, 71, pp.83~94 (2015)[DOI]
- Bar A, "Re-evaluation of calcium and phosphorus requirements in aged laying hens", Br Poult Sci, 43, pp.261~269 (2002)[DOI]
- Nkiambuo N, "Effects of supplementation with different levels of a multi strain probiotic on the performance of laying chicken", East Afr J Sci Technol Innov, 4, pp.2~ (2023)[DOI]
- Resta-Lenert S, "Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli(EIEC)", Gut, 52, pp.988~997 (2003)[DOI]
- Celi P, "Biomarkers of gastrointestinal functionality in animal nutrition and health", Anim Feed Sci Technol, 250, pp.9~31 (2019)[DOI]
- Wickramasuriya SS, "Role of physiology, immunity, microbiota, and infectious diseases in the gut health of poultry", Vaccines(Basel), 10, pp.172~ (2022)[DOI]
