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Effects of acute and chronic heat stress on the rumen microbiome in dairy goats

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Objective: The objective of this study was to reveal the influence of acute and chronic heat stress (HS) on the abundance and function of rumen microbiome and host metabolism.Methods: Forty mid-lactation goats were randomly divided into two artificial environments: control group and heat-stressed group. This study was recorded from two periods, 1 day and 28 days. The first day was defined as control 1 (CT1) and HS 1 (acute HS), and the last day was defined as CT28 and HS28 (chronic HS). On the first and last day, 6 dairy goats in each group were randomly selected to collect rumen liquid after the morning feeding through oral stomach tubes. The barn temperature and humidity were recorded every day.Results: Disruption of the rumen microbiome was observed under chronic HS, represented by an increase in the abundance of <i>Prevotella</i> and <i>Bacteroidales</i> (p<0.05), and upregulation of carbohydrate transport and metabolism functions (p<0.05). Additionally, the abundance of <i>Succinimonas</i> and <i>Ruminobacter</i> in chronic HS is lower than in acute HS (p<0.05), and the functions of intracellular trafficking, secretion and vesicular transport, and the cytoskeleton were downregulated (p<0.05).Conclusion: The HS affected the interaction between the microbiota and host, thereby regulated milk production in dairy goats. These findings increased understanding of the crosstalk between hosts and bacteria.

Abstract

Objective: The objective of this study was to reveal the influence of acute and chronic heat stress (HS) on the abundance and function of rumen microbiome and host metabolism. Methods: Forty mid-lactation goats were randomly divided into two artificial environments: control group and heat-stressed group. This study was recorded from two periods, 1 day and 28 days. The first day was defined as control 1 (CT1) and HS 1 (acute HS), and the last day was defined as CT28 and HS28 (chronic HS). On the first and last day, 6 dairy goats in each group were randomly selected to collect rumen liquid after the morning feeding through oral stomach tubes. The barn temperature and humidity were recorded every day. Results: Disruption of the rumen microbiome was observed under chronic HS, represented by an increase in the abundance of Prevotella and Bacteroidales (p<0.05), and upregulation of carbohydrate transport and metabolism functions (p<0.05). Additionally, the abundance of Succinimonas and Ruminobacter in chronic HS is lower than in acute HS (p<0.05), and the functions of intracellular trafficking, secretion and vesicular transport, and the cytoskeleton were downregulated (p<0.05). Conclusion: The HS affected the interaction between the microbiota and host, thereby regulated milk production in dairy goats. These findings increased understanding of the crosstalk between hosts and bacteria.

참고문헌 (29)

  1. Kim SH, "Heat stress : effects on rumen microbes and host physiology, and strategies to alleviate the negative impacts on lactating dairy cows", Front Microbiol, 13, pp.804562~ (2022)[DOI]
  2. Aleena J, "Resilience of three indigenous goat breeds to heat stress based on phenotypic traits and PBMC HSP70 expression", Int J Biometeorol, 62, pp.1995~2005 (2018)[DOI]
  3. Parida S, "Impact of heat stress on transcriptional abundance of HSP70 in cardiac cells of goat", Anim Biotechnol, 31, pp.223~228 (2020)[DOI]
  4. Pragna P, "Comparative assessment of growth performance of three different indigenous goat breeds exposed to summer heat stress", J Anim Physiol Anim Nutr(Berl), 102, pp.825~836 (2018)[DOI]
  5. Bohmanova J, "Temperature-humidity indices as indicators of milk production losses due to heat stress", J Dairy Sci, 90, pp.1947~1956 (2007)[DOI]
  6. Hou Y, "Comparing responses of dairy cows to short-term and long-term heat stress in climatecontrolled chambers", J Dairy Sci, 104, pp.2346~2356 (2021)[DOI]
  7. Tajima K, "Influence of high temperature and humidity on rumen bacterial diversity in Holstein heifers", Anaerobe, 13, pp.57~64 (2007)[DOI]
  8. Min L, "Metabolic responses and"omics"technologies for elucidating the effects of heat stress in dairy cows", Int J Biometeorol, 61, pp.1149~1158 (2017)[DOI]
  9. Zhong S, "Temperature and humidity index(THI)-induced rumen bacterial community changes in goats", Appl Microbiol Biotechnol, 103, pp.3193~3203 (2019)[DOI]
  10. Pinto S, "Critical THI thresholds based on the physiological parameters of lactating dairy cows", J Therm Biol, 88, pp.102523~ (2020)[DOI]
  11. Broderick GA, "A statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen", J Dairy Sci, 80, pp.2964~2971 (1997)[DOI]
  12. Liang YS, "Growth performance, rumen fermentation, bacteria composition, and gene expressions involved in intracellular pH regulation of rumen epithelium in finishing Hu lambs differing in residual feed intake phenotype", J Anim Sci, 95, pp.1727~1738 (2017)[DOI]
  13. Magoč T, "Flash : fast length adjustment of short reads to improve genome assemblies", Bioinformatics, 27, pp.2957~2963 (2011)[DOI]
  14. Edgar RC, "Uparse : highly accurate OTU sequences from microbial amplicon reads", Nat Methods, 10, pp.996~998 (2013)[DOI]
  15. Schloss PD, "Introducing mothur : open-source, platform-independent, community-supported software for describing and comparing microbial communities", Appl Environ Microbiol, 75, pp.7537~7541 (2009)[DOI]
  16. Caporaso JG, "QIIME allows analysis of high-throughput community sequencing data", Nat Methods, 7, pp.335~336 (2010)[DOI]
  17. Otasek D, "Cytoscape automation : empowering workflow-based network analysis", Genome Biol, 20, pp.185~ (2019)[DOI]
  18. Desnottes JF, "Effect of pefloxacin on microorganism : host cell interaction", J Antimicrob Chemother, 26(Suppl B), pp.17~26 (1990)[DOI]
  19. Xue MY, "Assessment of rumen bacteria in dairy cows with varied milk protein yield", J Dairy Sci, 102, pp.5031~5041 (2019)[DOI]
  20. Betancur-Murillo CL, "Prevotella : a key player in ruminal metabolism", Microorganisms, 11, pp.1~ (2022)[DOI]
  21. Tett A, "Prevotella diversity, niches and interactions with the human host", Nat Rev Microbiol, 19, pp.585~599 (2021)[DOI]
  22. Bass AR, "Comparative safety and effectiveness of TNF inhibitors, IL6 inhibitors and methotrexate for the treatment of immune checkpoint inhibitor-associated arthritis", Ann Rheum Dis, 82, pp.920~926 (2023)[DOI]
  23. Wang D, "Potential roles of the rectum keystone microbiota in modulating the microbial community and growth performance in goat model", J Anim Sci Biotechnol, 14, pp.55~ (2023)[DOI]
  24. Contreras-Jodar A, "Effects of chronic heat stress on lactational performance and the transcriptomic profile of blood cells in lactating dairy goats", J Dairy Res, 85, pp.423~430 (2018)[DOI]
  25. Bach A, "Changes in the rumen and colon microbiota and effects of live yeast dietary supplementation during the transition from the dry period to lactation of dairy cows", J Dairy Sci, 102, pp.6180~6198 (2019)[DOI]
  26. Wallace RJ, "The rumen microbial metagenome associated with high methane production in cattle", BMC Genomics, 16, pp.839~ (2015)[DOI]
  27. Cao N, "Calcium propionate supplementation alters the ruminal bacterial and archaeal communities in pre-and postweaning calves", J Dairy Sci, 103, pp.3204~3218 (2020)[DOI]
  28. Wang W, "MAPK4 promotes triple negative breast cancer growth and reduces tumor sensitivity to PI3K blockade", Nat Commun, 13, pp.245~ (2022)[DOI]
  29. Zhao C, "Sialic acid exacerbates gut dysbiosis-associated mastitis through the microbiota-gutmammary axis by fueling gut microbiota disruption", Microbiome, 11, pp.78~ (2023)[DOI]

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