사전 저온 수영이 MCAo 유발 쥐의 infarct volume 및 motor function에 미치는 영향
Preventive effects of swimming training in cold water on cerebral infarct volume and motor functions in MCAo rats
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이현정, 이정필, 김호성, 권기욱, 오재근. 사전 저온 수영이 MCAo 유발쥐의 infarct volume 및 motor function에 미치는 영향. 운동과학, 제14권 제3호, 417-428. 2005. 본 연구는 사전 저온 수영이 MCAo로 유발된 infarct volume 증가와 motor function 손실의 예방에 미치는 영향을 알아보기 위해 수행하였다. 42마리의 SD계 쥐들은 CS(저온수영, 24±1℃), NS(상온수영, 34±1℃), C(통제집단)으로 나눈 후 수술적 처치 방법에 따라 M(MCAo)와 S(Sham)로 배정되었다. 7일 동안 30분의 무부하 자유수영을 완료한 쥐들은 다시 MCAo 및 Sham 유발을 위한 수술처치를 실시하였으며, 수술처치 4일 후(실험시작 11일 후) 모든 실험동물을 희생시켰다. 적출 후 절편된 뇌조직은 Infarct volume을 평가하기 위해 TTC 염색을, VEGF 발현을 평가하기 위해 western blotting을 실시하였다. motor function test(foot fault test, balance beam test and limb placing test)는 수술처치 3일 후에 실시하였다. MCS의 infarct volume이 MC에 비해 유의하게 감소하였다. VEGF 발현은 저온 수영 집단에서 유의하게 증가하였으며, MCS와 MNS에 있어서도 각각 SCS와 SNS에 비해 유의한 증가를 보였다. 대부분의 motor function test 결과 사전 수영 훈련이, 특히 저온 수영 훈련, motor function의 감소를 억제하는 것으로 나타났다. 이상의 결론에 비추어, MCAo 유발 전에 수행된 저온 수영 훈련은 VEGF 발현을 증가시켜 infarct volume의 감소와 motor function의 결손을 예방할 수 있을 것으로 사료된다.
Abstract
Lee, H.J., Lee, J.P., Kim, H.S., Kwon, K.U., Oh, J.K. Preventive effects of swimming training in cold water on cerebral infarct volume and motor functions in MCAo rats. Exercise Science, 14(3): 417-428, 2005. The purpose of this study was to determine the preventive effects of previous swimming training in cold water on cerebral infarct volume and motor functions caused by middle cerebral artery occlusion(MCAo) in male rats. 42 Rats were divided into three groups: CS(swimming training in cold water, 24±1℃), NS(swimming training in normal temperature water, 34±1℃), C(control), and then divided into two groups by surgical treatment: M(MCAo) and S(Sham). For 7d before surgical treatments, free weight swimming during 30 min/d were completed. 4d after MCAo, brain were prepared for TTC staining and western blotting to assess infarct volume and Vascular endothelial growth factor(VEGF) expression. Foot fault test, balance beam test and limb placing test were conducted to access motor functions at 3d after surgical treatments. The infarct volumes of MCS was significantly decreased than MC. The VEGF expression was significantly increased by swimming training in cold water whereas the VEGF expression was significantly increased in MCAo group(MCS and MNS) compared to Sham(SCS and SNS). The result of the motor function tests showed that pre-swimming training, especially in cold water, inhibit decreasing motor functions in MCAo. In conclusion, increased VEGF expression induced by swimming in cold water before MCAo, can prevent increase of infarct volume and deficiency of motor functions.
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