Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor
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We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE)removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2-dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at 0.006 mg protein−1 · l · h−1, and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from 30 μM to 905 μM, the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be 1,100 μmol · d−1l of reactor volume−1, which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.
Abstract
We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE)removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2-dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at 0.006 mg protein−1 · l · h−1, and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from 30 μM to 905 μM, the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be 1,100 μmol · d−1l of reactor volume−1, which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.
참고문헌 (58)
- Kazuhiro Takamizawa, "Evaluation of Different Culture Conditions of Clostridium bifermentans DPH-1 for Cost Effective PCE Degradation", Biotechnology and Bioprocess Engineering, 10(1), pp.40~46 (2005), 한국생물공학회
- Aulenta, F, "Complete dechlorination of tetrachloroethene to ethane in presence of methanogenesis and acetogenesis by an anaerobic sediment microcosm", Biodegradation, 13, pp.411~424 (2002)
- Aulenta, F, "Anaerobic transformation of tetrachloroethane, perchloroethylene, and their mixtures by mixed-cultures enriched from contaminated soils and sediments", Water Science and Technology, 52, pp.357~362 (2005)
- Aulenta, F, "Influence of hydrogen on the reductive dechlorination of tetrachloroethene (PCE) to ethene in a methanogenic biofilm reactor: Role of mass transport phenomena", Journal of Chemical Technology & Biotechnology, 81, pp.1520~1529 (2006)
- Brewster, C, "A PCE groundwater plume discharging to a river:influence of the streambed and near-river zone on contaminant distributions", Journal of Contaminant Hydrology, 73, pp.249~279 (2004)
- Cabirol, N, "Complete degradation of high concentrations of tetrachloroethylene by a methanogenic consortium in a fixed-bed reactor", Journal of Biotechnology, 62, pp.133~141 (1998)
- Carter,S.R, "Biotransformation of tetrachloroethylene by anaerobic attached-films at low temperatures", Water Research, 27, pp.607~615 (1993)
- Chang, Y. C, "Isolation and characterization of a tetrachloroethylene dechlorinating bacterium, Clostridium bifermentans DPH-1", Journal of Bioscience and Bioengineering, 89, pp.489~491 (2000)
- Cole, J. R, "Tetrachloroethene and 3-chlorobenzoate dechlorination activities are co-induced in Desulfomonile tiedje DCB-1", Biodegradation, 6, pp.167~172 (1995)
- Christiansen, N, "Transformation of tetrachloroethene in an upflow anaerobic sludgeblanket reactor", Applied Microbiology and Biotechnology, 47, pp.91~94 (1997)
- Cupples, A. M, "Comparative evaluation of chloroethene dechlorination to ethene by Dehalococcoides-like microorganisms", Environmental Science & Technology, 38, pp.4768~4774 (2004)
- Damborsky, J, "Tetrachloroethene-Dehalogenating Bacteria", Folia Microbiologica, 44, pp.247~262 (1999)
- DiStefano, T. D, "Reductive dechlorination of high concentration of tetrachloroethene to ethane by an anaerobic enrichment culture in the absence of methanogenesis", Applied and Environmental Microbiology, 57, pp.2287~2292 (1991)
- deBruin, W. P, "Complete biological reductive transformation of tetrachloroethene to ethane", Applied and Environmental Microbiology, 58, pp.1996~2000 (1992)
- Eisenbeis,M, "Studies on the dechlorination of tetrachloroethene to cis-1,2-dichloroethene by Dehalospirillum multivorans in biofilms", Water Science and Technology, 36, pp.191~198 (1997)
- Ensley, B. D, "Biochemical diversity of trichlo roethylene metabolism", Annual Review of Microbiology, 45, pp.283~299 (1991)
- Fathepure, B. Z, "Anaerobic bacteria that dechlorinate perchloroethene", Applied and Environmental Microbiology, 53, pp.2671~2674 (1987)
- Fathepure,B.Z, "Complete degradation of polychlorinated hydrocarbons by a twostage biofilm reactor", Applied and Environmental Microbiology, 57, pp.3418~3422 (1991)
- Fetzener, S, "Bacterial dehalogenation", Applied Microbiology and Biotechnology, 50, pp.633~657 (1998)
- Freedman,D.L, "Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions", Applied and Environmental Microbiology, 55, pp.2144~2151 (1989)
- Flynn, S. J, "Microbial community changes association with a shift from reductive degradation of PCE to reductive degradation of cis-DCE and VC", Environmental Science & Technology, 34, pp.1056~1061 (2000)
- Gerritse, J, "Complete degradation of tetrachloroethene by combining anaerobic dechlorinating and aerobic methanotrophic enrichment cultures", Applied Microbiology and Biotechnology, 43, pp.920~928 (1995)
- Gerritse, J, "Complete degradation of tetrachloroethene in coupled anoxic and oxic chemostats", Applied Microbiology and Biotechnology, 48, pp.553~562 (1997)
- Hirl,P.J, "Reductive dechlorination of perchloroethylene using anaerobic sequencing batch biofilm reactors (AnSBBR)", Water Science and Technology, 35, pp.49~56 (1997)
- Hoelen, T. P, "Bioremediation of cis-DCE at a sulfidogenic site by amendment with propionate", Ground Water Monitoring and Remediation, 26, pp.82~91 (2006)
- Holliger, C, "A high purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth", Applied and Environmental Microbiology, 59, pp.2991~2997 (1993)
- Höbber, C, "Improved dechlorinating incorporation of Dehalospirillum multivorans into granular sludge", Applied and Environmental Microbiology, 64, pp.1860~1863 (1998)
- Isalou, M, "Biodegradation of high concentrations of tetrachloroethene in a continuous flow column system", Environmental Science & Technology, 32, pp.3579~3585 (1998)
- Hata, J, "Anaerobic degradation of cisdichloroethylene and vinyl chloride by Clostridium sp. strain DC1 isolated from landfill leachate sediment", Journal of Bioscience and Bioengineering, 97, pp.196~201 (2004)
- Komatsu,T, "Reductive transformation of tetrachloroethy1ene to ethylene and ethane by an anaerobic filter", Water Science and Technology, 36, pp.125~132 (1997)
- Koziollet, P. D, "Ethene as an auxiliary substrate for the cooxidation of cis-1,2-dichloroethene and vinyl chloride", Archives of Microbiology, 172, pp.240~246 (1999)
- Lee, T. H, "Efficient dechlorination of tetrachloroethylene in soil slurry by combined use of an anaerobic Desulfitobacterium sp. strain Y-51 and zero-valent iron", Journal of Bioscience and Bioengineering, 92, pp.453~458 (2001)
- Lowry, O. H, "Protein measurement with the folin phenol reagent", Journal of Biological Chemistry, 193, pp.265~275 (1951)
- Ma, X, "Evaluation of hollow-fiber polyethylene membranes for hydrogen delivery to support reductive dechlorination in a soil column", Water Research, 37, pp.2905~2918 (2003)
- Ma, X, "Comparison of pulsed and continuous addition of H2 gas via membranes for stimulating PCE biodegradation in soil columns", Water Research, 40, pp.1155~1166 (2006)
- Malachowsky, K. J, "Aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by Rhodococcus species", Applied and Environmental Microbiology, 60, pp.542~548 (1994)
- Maymó-Gatell, X, "Characterization of an H2-utilizing enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethene in the absence of methanogenesis and acetogenesis", Applied and Environmental Microbiology, 61, pp.3928~3933 (1995)
- Maymó-Gatell, X, "Isolation of a bacterium that reductively dechlorination tetrachloroethene to ethane", Science, 276, pp.1568~1571 (1997)
- Maymó-Gatell, X, "Reductive dechlorination of cis-1,2-dichloroethene and vinyl chloride by “Dehalococcoides ethenogenes", Environmental Science & Technology, 35, pp.516~521 (2001)
- McCarty, P. L, "Breathing with chlorinated solvents", Science, 276, pp.1521~1522 (1997)
- Mendoza-Sanchez, I, "Effect of pore velocity on biodegradation of cis-dichloroethene (DCE) in column experiments", Biodegradation, 21, pp.365~377 (2010)
- Muenzner, H. D, "Dechlorination of PCE by mixed methanogenic cultures using hollow- fiber membranes", Bioremediation Journal, 6, pp.337~350 (2002)
- Noftsker,C, "Removal of tetrachloroethylene in an anaerobic column bioreactor", Applied Microbiology and Biotechnology, 48, pp.424~430 (1997)
- Parsons, F, "Transformation of tetrachloroethane in microcosms and groundwater", Journal of the American Water Works Association, 76, pp.56~59 (1984)
- Parakash,S.M, "Biodegradation of tetrachloroethylene in upflow anaerobic sludge blanket reactor", Bioresource Technology, 72, pp.47~54 (2000)
- Rosenthal,H, "Dechlorination of PCE in the presence of Fe0 enhanced by a mixed culture containing two Dehalococcoides strains", Chemosphere, 55, pp.661~669 (2004)
- Ryoo, D, "Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stuzeri OX1", Nature Biotechnology, 18, pp.775~778 (2000)
- Schmidt,J.E, "Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors", Biotechnology and Bioengineering, 49, pp.229~249 (1996)
- Scholz-Muramatsu, H, "Tetrachloroethylene as electron acceptor for the anaerobic degradation of benzoate", FEMS Microbiology Letters, 66, pp.81~86 (1990)
- Scholz-Muramatsu, H, "Isolation and characterization of Dehalospirillum multivorans gen.nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium", Archives of Microbiology, 163, pp.48~56 (1995)
- Sharma,O.K, "Isolation and characterization of a facultative aerobic bacterium that reductively dehalogenates tetrachloroethene to cis-1,2-dichloroethene", Applied and Environmental Microbiology, 62, pp.761~765 (1996)
- Suyama, A, "Isolation and characterization of Desulfitobacterium sp. strain Y51 capable of efficient dechlorination of tetrachloroethene and polychloroethanes. Bioscience", Biotechnology,and Biochemistry, 65, pp.1474~1481 (2001)
- "U.S. EPA : Superfund NPL Characterization Project: National Results", National Results. U.S. Environmental Protection Agency, Office of Emergency and Remedial Response, ~ (1991)
- "U.S. EPA : National revised primary drinking water regulations, volatile synthetic organic chemicals in drinking water: advanced notice of proposed rulemaking", pp.9349~9358 (1982), Federal Register
- Vogel,T.M, "Biotransforination of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under methanogenic conditions", Applied and Environmental Microbiology, 49, pp.1080~1083 (1985)
- Wild,A.P, "Anaerobic dech1orination of trichloroethene, tetrachloroethene and 1,2-dichloroethane by an acetogenic mixed culture in a fixed-bed reactor", Biodegradation, 6, pp.309~318 (1995)
- Wu, W. M, "Dechlorination of PCE and TCE to ethene using an anaerobic microbial consortium. In: Bioremediation of chlorinated solvents. Hinchee, R.E., Leeson, A. and Semprini, L", pp.45~52 (1995), Battelle, Columbus Richland
- Yamamoto, K, "Contamination of vinyl chloride in shallow urban river in Osaka, Japan", Water Research, 35, pp.561~566 (2001)