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논문

학교 집단급식소 내 식기류 및 집기류의 미생물학적 분석 및 위해요인 평가

A Microbiological Analysis and Hazard Factor Evaluation of Food Utensils and Fixtures of Food Service Operations in Schools

고광표|홍성호|박성준|이하영|김철주|김수진|김성균
한국환경보건학회 (Korean Society of Environmental Health)|2011.10|한국환경보건학회지 (JOURNAL OF ENVIRONMENTAL HEALTH SCIENCES)|제37권 제5호|pp. 376-386

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참고문헌

키워드

SchoolMicroorganismFood servicesEating utensil

초록

Objectives: The aim of this study was to evaluate the microbial hazards posed by food utensils and fixtures in food service operations at selected middle and high schools located in Seoul, Korea. Methods: We collected 200 samples of utensils and fixtures including cups, spoons/chopsticks, food trays and tables from five different schools in Seoul. Target microorganisms of this study were divided into two groups: total bacterial count and total coliform as indicators of microbial contamination and Bacillus cereus and Staphylococcus aureus as pathogens of food poisoning. We used selective media to quantify microbial concentration and 16S rRNA PCR assay for qualitative analysis. In addition, intensive interviews with nutritionists were conducted and observations were made to identify factors that may affect microbial contamination. Logistic regression analysis was employed to examine the relationship between the microbial concentration and operation characteristics of each operation. Results: The level of microbial concentration in school B and C were significantly lower than in school A, D and E (p<0.05). Some samples from school A, D and E showed over 3.4 log CFU/100 cm2 (total bacterial count) and 1.0 log CFU/100cm2 (total coliform), which requires immediate hygienic action. The number of customers per staff member, periodicity of hygiene education for staff and daily operation time of sterilizers were also found to be important factors related with the microbial contamination of food service operations. Conclusions: These results suggested that not only a HACCP (Hazard Analysis and Critical Control Point) approach, but also efforts to assess internal risk factors within operations be needed to reduce the microbial contamination of food utensils and fixtures. This study is expected to provide preliminary data for assessing microbial hazards in food service operations.

Abstract

Objectives: The aim of this study was to evaluate the microbial hazards posed by food utensils and fixtures in food service operations at selected middle and high schools located in Seoul, Korea. Methods: We collected 200 samples of utensils and fixtures including cups, spoons/chopsticks, food trays and tables from five different schools in Seoul. Target microorganisms of this study were divided into two groups: total bacterial count and total coliform as indicators of microbial contamination and Bacillus cereus and Staphylococcus aureus as pathogens of food poisoning. We used selective media to quantify microbial concentration and 16S rRNA PCR assay for qualitative analysis. In addition, intensive interviews with nutritionists were conducted and observations were made to identify factors that may affect microbial contamination. Logistic regression analysis was employed to examine the relationship between the microbial concentration and operation characteristics of each operation. Results: The level of microbial concentration in school B and C were significantly lower than in school A, D and E (p<0.05). Some samples from school A, D and E showed over 3.4 log CFU/100 cm2 (total bacterial count) and 1.0 log CFU/100cm2 (total coliform), which requires immediate hygienic action. The number of customers per staff member, periodicity of hygiene education for staff and daily operation time of sterilizers were also found to be important factors related with the microbial contamination of food service operations. Conclusions: These results suggested that not only a HACCP (Hazard Analysis and Critical Control Point) approach, but also efforts to assess internal risk factors within operations be needed to reduce the microbial contamination of food utensils and fixtures. This study is expected to provide preliminary data for assessing microbial hazards in food service operations.

참고문헌 (24)

  1. 김종규, "일부 학교급식소 조리실의 위생관리에 관한 조사연구", 한국환경보건학회지, 29(2), pp.87~93 (2003), 한국환경보건학회
  2. "Current Status of School Foodservice programs in 2010"[URL]
  3. "The Present Status of student rights since 2008"[URL]
  4. 김경열, "대학 내 급식소의 안전성 확보를 위한 미생물학적 안전성 평가", 한국식품위생안전성학회지, 25(1), pp.49~58 (2010), 한국식품위생안전성학회
  5. 송임숙, "제주지역 학교급식 조리종사자의 HACCP 관련 지식 및 수행도 분석", 한국영양학회지, 41(8), pp.870~886 (2008), 한국영양학회
  6. "The System of Food Poisoning Statistics" (2010)[URL]
  7. 황승숙, "학교급식 운영형태별 위생·안전 및 HACCP 시스템", 한국생활환경학회지, 15(3), pp.372~382 (2008), 한국생활환경학회
  8. Mokhtari A, "Quantitative Exposure Model for the Transmission of Norovirus in Retail Food Preparation", Int J Food Microbiol, 133(1), pp.38~47 (2009)
  9. "Current Status of Sanitation of Food Courts" (2011)[URL]
  10. 정동관, "초등학교 단체급식소의 환경과 급식설비에 대한 미생물 평가", 한국식품영양과학회지, 31(2), pp.216~220 (2002), 한국식품영양과학회
  11. Torrence ME, "Microbial Food Safety in Animal Agriculture: Current Topics", pp.275~280 (2003), Wiley- Blackwell
  12. 박정영, "조리종사자의손씻기의식과실천및손의지표미생물오염도에관한연구", 한국환경보건학회지, 36(2), pp.95~107 (2010), 한국환경보건학회
  13. Ministry of Education, Science and Technology, Korea, "Guidelines of Sanitation Management for School Foodservice Operations", 3, pp.1~130 (2010)
  14. "Bacteriological Analytical Manual"[URL]
  15. Marchesi JR, "Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA", Appl Environ Microbiol, 64(2), pp.795~799 (1998)
  16. Sites JE, "Development and Validation of a Pilot Scale Enhanced Biosafety Level Two Containment for Performance Evaluation of Produce Disinfection Technologies", Applied Biosafety, 13(1), pp.30~44 (2008)
  17. Harrigan WF, "The examination of food processing plant. In Laboratory methods in food and dairy microbiology", pp.231~236 (1976), Academic Press
  18. Ehrlich R, "Relationship Between Atmospheric Temperature and Survival of Airborne Bacteria", Appl Microbiol, 19(2), pp.245~249 (1970)
  19. Jawad A, "Influence of relative humidity and suspending menstrua on survival of Acinetobacter spp. on dry surfaces", J Clin Microbiol, 34(12), pp.2881~2887 (1996)
  20. 박상현, "대전,충남지역 학교급식의 위생관리 실태조사", 대한지역사회영양학회지, 10(2), pp.234~242 (2005), 대한지역사회영양학회
  21. 김종규, "맨손과 장갑낀 손의 미생물 오염도 비교", 한국환경보건학회지, 37(4), pp.298~305 (2011), 한국환경보건학회
  22. 배현주, "대학급식소 고객의 손 위생에 대한 미생물학적 위해 평가", 한국식품영양과학회지, 35(7), pp.940~944 (2006), 한국식품영양과학회
  23. Kjølen H, "Handwashing and disinfection of heavily contaminated hands-effective or ineffective", J Hospital Infect, 21(1), pp.61~70 (1991)
  24. 박정영, "조리종사자의손씻기의식과실천및손의지표미생물오염도에관한연구", 한국환경보건학회지, 36(2), pp.95~107 (2010), 한국환경보건학회

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