Non-invasive Biological Monitoring of DNA Adducts Formed at Workers Handling 3,3’-dichlorobenzidine(DCB) by Using GC/MS

Abstract

We examine the metabolites(DCB and acetyl DCB) extracted from exfoliated urothelial cells of 33 workers who employed DCB-handling industries. The characteristics of workers submitted urine, whose age, working years and smoking persons were 41.911.1, 8.75.5 and 25(32.0%), respectively. DNA adduct was isolated from the exfoliated urothelial cells by applying 32p-postlabeling procedure. Metabolites(DCB and acetyl DCB) were extracted from DNA adducts by hydrolyzing and N-glycosylase. Concentrations of DCB and acetyl DCB were 28.65.25 ng/g DNA, and 17.03.73 ng/g DNA, respectively. The regression between DCB level and exposure years of workers is y=1.668+2.588x(p=0.005, r2=0.394). The regression between acetyl DCB level and exposure years of workers is y=8.071+1.325x(p=0.076, r2=0.222). Smoking workers are significantly higher than non-smoking workers on DCB and acetyl DCB level(p=0.065 and 0.021, respectively). DCB level was 33.97.14 ng/g DNA on smokers, and 23.19.97 ng/g DNA on non-smokers. Acetyl DCB was 25.15.27 ng/g DNA on smokers, and 8.927.22 ng/g DNA on non-smokers.