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가시오가피 에탄올추출물의 AGS위암세포주에서 세포주기억제효과

The antitumor activities of Acanthopanax senticosus Harms(ASH) in human gastric cancer AGS cell lines

고성규|신용철|신헌태|이선동
대한예방한의학회 (Society of Preventive Korean Medicine)|2011.12|대한예방한의학회지 (Society of Preventive Korean Medicine)|제15권 제3호|pp. 127-140

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참고문헌

키워드

Acanthopanax senticosus Harms(ASH)Gastric cancercell cycleapoptosisEGFR-STAT3 pathway
주제분류한의학

초록

Objectives : The research was conducted to confirm the effect of Acanthopanax senticosus harms(ASH)on the anti-tumor activities in AGS human gastric cancer cells. Methods : To examine the potential anti-tumor effect of ASH, we performed many experiments. After processing AGS cancer cells with varying concentrations 80% ethanol ASH extract, analyses by MTT,flow cytometer(FACS) and western blot were used. Results : AGS cancer cells showed decreased cell proliferation and increased contents of S phase when treated with ASH. Moreover, the Western blot experiment showed that ASH affected S phase cell cycle-related molecules(Cyclin A, p21 and p16) in AGS cells. ASH also inhibited EGFR-STAT3 pathway in AGS human gastric cancer cells. Conclusion : Based on these results, we observed that ASH arrested the cell cycle at S phase and inhibited the phosphorylation of EGFR and STAT3 proteins which reduce the cell cycle and the manifestation of the genes that are related to inhibiting cell growth in AGS cells. It can be concluded that ASH can be used in developing medicine for gastric cancer.

Abstract

Objectives : The research was conducted to confirm the effect of Acanthopanax senticosus harms(ASH)on the anti-tumor activities in AGS human gastric cancer cells. Methods : To examine the potential anti-tumor effect of ASH, we performed many experiments. After processing AGS cancer cells with varying concentrations 80% ethanol ASH extract, analyses by MTT,flow cytometer(FACS) and western blot were used. Results : AGS cancer cells showed decreased cell proliferation and increased contents of S phase when treated with ASH. Moreover, the Western blot experiment showed that ASH affected S phase cell cycle-related molecules(Cyclin A, p21 and p16) in AGS cells. ASH also inhibited EGFR-STAT3 pathway in AGS human gastric cancer cells. Conclusion : Based on these results, we observed that ASH arrested the cell cycle at S phase and inhibited the phosphorylation of EGFR and STAT3 proteins which reduce the cell cycle and the manifestation of the genes that are related to inhibiting cell growth in AGS cells. It can be concluded that ASH can be used in developing medicine for gastric cancer.

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