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益氣養榮湯의 항산화 및 유방암 세포주 생장 억제 효과에 미치는 영향

Antioxidative and Anti-proliferative Effects on MCF-7 Human Breast Cancer Cells of Ikiyangyoung-Tang

조성희|박경미|이진아
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2007.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제20권 제1호|pp. 33-46

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키워드

cytotoxicityMCF-7human breast cancerIkiyangyoung-Tang antiproliferationantioxidative effect
주제분류한의학

초록

Purpose: Breast cancer is the most common disease in Korean women. Despite remarkable improvements in treatment strategies against various cancer during the past 40 years, breast cancer still remains as one of the main causes of cancer mortality among women in the whole world. This study was carried out to investigate antioxidative and anti-proliferative effects on MCF-7 human breast cancer cells of Ikiyangyoung-Tang extract. Methods: We measured a content of polyphenol and flavonoid in the Ikiyangyoung-Tang extract, eliminative ability of DPPH radical, ABTS free radical and hydrogen peroxide, antioxidative effects of linoleic acid, cytotoxicity on MCF-7 human breast cancer cells. MCF-7 cells were cultured in Dulbecco's modified Eagle's medium/F12(DMEM/F12) supplemented with 10 % fetal bovine serum(FBS; Gibco) and antibiotics. Results: The extract of Ikiyangyoung-tang contains polyphenol of 168.3±12.8 ㎍/㎎ and flavonoid of 84.3±3.4 ㎍/㎎. Above results show profitable abilities of elimination of α-α-Diphenyl-β-picrylhydrazyl(DPPH) radical, ABTS free radical and hydrogen peroxide. Also, the extract of Ikiyangyoung-Tang strongly inhibits the proliferation of MCF-7 cells in a dose ependent manner. And, it has cytotoxicity on NIH3T3 cells. Conclusion: It can be concluded that Ikiyangyoung-Tang extract has an antioxidative effect and antiproliferative effect on MCF-7 human breast cancer cells.

Abstract

Purpose: Breast cancer is the most common disease in Korean women. Despite remarkable improvements in treatment strategies against various cancer during the past 40 years, breast cancer still remains as one of the main causes of cancer mortality among women in the whole world. This study was carried out to investigate antioxidative and anti-proliferative effects on MCF-7 human breast cancer cells of Ikiyangyoung-Tang extract. Methods: We measured a content of polyphenol and flavonoid in the Ikiyangyoung-Tang extract, eliminative ability of DPPH radical, ABTS free radical and hydrogen peroxide, antioxidative effects of linoleic acid, cytotoxicity on MCF-7 human breast cancer cells. MCF-7 cells were cultured in Dulbecco's modified Eagle's medium/F12(DMEM/F12) supplemented with 10 % fetal bovine serum(FBS; Gibco) and antibiotics. Results: The extract of Ikiyangyoung-tang contains polyphenol of 168.3±12.8 ㎍/㎎ and flavonoid of 84.3±3.4 ㎍/㎎. Above results show profitable abilities of elimination of α-α-Diphenyl-β-picrylhydrazyl(DPPH) radical, ABTS free radical and hydrogen peroxide. Also, the extract of Ikiyangyoung-Tang strongly inhibits the proliferation of MCF-7 cells in a dose ependent manner. And, it has cytotoxicity on NIH3T3 cells. Conclusion: It can be concluded that Ikiyangyoung-Tang extract has an antioxidative effect and antiproliferative effect on MCF-7 human breast cancer cells.

참고문헌 (39)

  1. "한국중앙암등록사업 연례 보고서" (2003)
  2. "부인과학 제3판", pp.1299~1301 (1997), 서울:칼빈서적
  3. "한의부인과학 상", pp.364~366 (2001), 서울:정담
  4. "유방암의 방사선치료", 21(1), pp.37~ (2005)
  5. "유방종괴에 관한 문헌적 고찰", 11(2), pp.29~43 (1998)
  6. "유방암의 다용 약물에 대한 문헌적 고찰", 9(2), pp.57~86 (2001)
  7. "청간해울탕이 소염, 진통, 면역세포 및 유방암 세포에 미치는 영향", 10(2), pp.69~83 (1997)
  8. "SKBR3 유방암세포주에 대한 귀전우 메탄올 추출물의 성장 억제 및 항산화효과", 18(1), pp.45~54 (2005)
  9. "황금의 유방암 세포주에 대한 항암작용", 25(3), pp.451~460 (2004)
  10. "반지련이 유방암에 미치는 영향에 관한 연구", 12(2), pp.148~182 (1999)
  11. "봉독약침액에 의한 인체 유방암세포의 성장억제 및 세포에 관한연구", 20(3), pp.45~62 (2003)
  12. "Official method of analysis Association of Official Analytical Chemists" (1985)
  13. "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", 71, pp.109~114 (2000)
  14. "Antioxidant activity applying an improved ABTS radical cation decolorization assay", 26, pp.1231~1237 (1999)
  15. "Detection of hydrogen peroxide produced by microorganism on ABTS- peroxidase medium", pp.151~158 (1985)
  16. "Antioxidative substances in leaves of Polygonum hydropiper", pp.1349~1351 (1992)
  17. "The activity of antioxidants and suppression of cancer cell proliferation in extracts of Orostachys japonicus A. Berger", 11(1), pp.31~39 (2003)
  18. "Cytotoxicity of Ethanol Extract of Raphanuse Sativus on a Human Lung Cancer Cell Line", 33(2), pp.287~290 (2004)
  19. "Cytotoxicity and Multidrug-Resistance Reversing Activity of Extracts from Gamma- Irradiated Coix lachryma-jobi L. Var. ma-yuen Stapf seed", 34(5), pp.613~618 (2005)
  20. "Cytotoxicity and Antimicrobial Effects of Extracts from Salvia miltiorrhiza", (4), pp.293~296 (2003)
  21. "Enhanced lmmune Activity and Cytotoxicity of Artemisia capillaris Thunb. Extracts against Human Cell Line", 12(1), pp.36~42 (2004)
  22. "Screening and evaluation of anticancer agents", 10(2), pp.67~ (1998)
  23. "Antimutagenic and Cytotoxicity Effects of Extracts of Eleutheroccus senticosus Maxim fruits", 10(3), pp.394~400 (2003)
  24. "Anticancer and Immune Activities of the Extracts from Amorpha fruticosa L", 13(1), pp.41~47 (2005)
  25. "Inhibitory Effects on Cell Survival and Quinone Reductase Induced Activity of Aster yomena Fractions on Human Cancer Cells", 34(1), pp.8~12 (2005)
  26. "Cytotoxicity and Antimicrobial Effect of the Extract of Salvia plebeia" (2001)
  27. "전이된 유방암 환자에 대한 미슬토를 사용한 면역요법의 임상례", 14(3), pp.209~217 (2001)
  28. "항암단을 투여한 유방암환자 60예에 대한 임상보고", 22(4), pp.669~674 (2001)
  29. "가미쌍화탕의 유방암 발생 및 전이 억제에 대한 실험적 연구", 11(2), pp.108~112 (1997)
  30. "活絡效靈丹 추출물의 인간 유방암세포 MCF-7에 대한 생장억제 효과", 19(3), pp.13~24 (2006)
  31. "三稜 추출물의 인간 유방암 세포 생장억제 효과", 19(1), pp.166~177 (2006)
  32. "歸朮破湯 추출물의 인간 유방암 세포에 대한 생장억제 효과", 19(1), pp.155~165 (2006)
  33. "the main constitutent of tannin in green tea", pp.44~47 (1987)
  34. "Antioxidant effects and application as natural ingredients of Korean sanguisorbae officinalis L", 47, pp.244~250 (2004)
  35. "Total antioxidant status in plasma and body fluids", pp.279~293 (1994)
  36. "A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates", pp.407~412 (1993)
  37. "Structure-antioxidant activity relationships of flavonoids and phenolic acids", 20, pp.933~956 (1996)
  38. "Antioxidatives activities of Korean medicinal plants", 11, pp.127~134 (2003)
  39. "Antioxidant activity of some spice essential oils on linoleic and oxidation in aqueous media", pp.792~799 (1989)

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