芎歸調血飮이 흰쥐의 자궁내막증 형성 억제에 미치는 영향
Inhibiting Effecposets of Gungguijohyeoleum on the Development of Experimentally-induced Endometriosis in the Rats
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Abstract
Purpose: This study was performed to investigate the inhibiting effects of Gungguijohyeoleum on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and we divided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and Gungguijohyeoleum treated group(n=8). Gungguijohyeoleum was orally administrated for 15 days after operation. Then we measured the body weight, the volumes of endometriotic implants, the weight of uterus and ovary, and investigated the concentrations of cytokines(MCP-1, TNF-α, IL-1β, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were performed. Results: - The volume (㎣) of endometriotic implants in Gungguijohyeoleum treated group (76.4±24.5) was significantly decreased(p<0.05) compared with control group(222.1±109.1). - The concentration (pg/㎖) of MCP-1 in peritoneal fluids in Gungguijohyeoleum treated group (1060.8±280.8) was significantly decreased(p<0.05) compared with control group(1412.5±345.7). - The concentration (pg/㎖) of TNF-α in peritoneal fluids in Gungguijohyeoleum treated group (827.5±219.9) was significantly decreased(p<0.01) compared with control group(1126.2±139.9). - The concentration (pg/㎖) of IL-1β in peritoneal fluids in Gungguijohyeoleum treated group(83.4±9.0) was significantly decreased(p<0.01) compared with control group(105.3±17.6). - The concentration (pg/㎖) of IL-6 in peritoneal fluids in Gungguijohyeoleum treated group(108.9±15.7) was decreased compared with control group(122.8±19.3). - Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Gungguijohyeoleum treated group were weakly observed than those of control group. - The percentage(%) of positive epithelial layers for COX-2 in Gungguijohyeoleum treated group(56.8±12.8) was significantly decreased(p<0.01) compared with control group (75.1±16.3). -The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Gungguijohyeoleum treated group was weakly observed than that of control group. - The numbers of mast cells in transplanted uterine tissue in Gungguijohyeoleum treated group(35.4±18.4) were significantly decreased(p<0.01) compared with control group (91.0±28.3). Conclusion: On the basis of these results, we concluded that Gungguijohyeoleum has inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, TNF-α, IL-1β, COX-2, VEGF and mast cells by administration of Gungguijohyeoleum.
참고문헌 (82)
- 한의부인과학 교재편찬위원회, "한의부인과학 상권", pp.186~190 (2002), 도서출판 정담
- Shakiba K, "Tumor necrosis factor-α blockers : potential limitations in the management of advanced endometriosis", Human Reproduction, 21(9), pp.2417~2420 (2006)
- Iwabe T, "Tumor necrosis factor-α promotes proliferation of endometriotic stromal cells by enducing Interleukin-8 gene and protein expression", The Journal of Clinical Endocrinology & Metabolism, 85(2), pp.824~829 (2000)
- Oral E, "The peritoneal environment in endometriosis", Human Reproduction Update, 2(5), pp.385~398 (1996)
- 대한산부인과학회, "부인과학", pp.566~597 (1997), 칼빈서적
- Nasir L, "Management of pelvic pain from dysmenorrhea or endometriosis" (2004), JABFP
- 김철수, "膈下逐瘀湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향", 대한한방부인과학회지, 20(1), pp.61~76 (2007), 대한한방부인과학회
- 임용택, "골반성 자궁내막증 환자에서의 Nafarelin Acetate(Synarel) 및 Danazol의 임상적인 치료효과", 대한산부회지, 34(12), pp.1716~1724 (1991)
- 이태균, "子宮內膜症에 대한 文獻的 考察", 대한한방부인과학회지, 8(1), pp.161~174 (1995)
- 자궁내막증의 새로운 진단, 치료 및 예방법 개발-1998년도 보건의료 기술연구, 개발사업 최종보고서, "보건복지부", 보건복지부, 2~108 (1999)
- 김동일, "子宮內膜症의 韓醫學 治療에 관한 硏究", 대한한방부인과학회지, 16(2), pp.126~143 (2001)
- 김여태, 서일복, 김형준, 이동녕, "加味歸朮破?湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향", 대한한방부인과학회지, 19(2), pp.162~185 (2006), 대한한방부인과학회
- 김형일, 권기록, 임은미, "溫經湯이 자궁내막증 유발 백서에 미치는 영향", 대한한방부인과학회지, 19(2), pp.18~33 (2006), 대한한방부인과학회
- 윤영희, "少腹逐瘀湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향", 대한한방부인과학회지, 19(2), pp.141~161 (2006), 대한한방부인과학회
- 장성환, "桃紅四物湯이 子宮內膜症을 유발한 흰쥐에 미치는 影響", 대한한방부인과학회지, 19(1), pp.125~138 (2006), 대한한방부인과학회
- 이지연, "加味紅藤湯 직장 내 투여가 자궁내막증을 유발한 흰쥐에 미치는 영향", 대한한방부인과학회지, 19(3), pp.109~120 (2006), 대한한방부인과학회
- 육상숙, "중성어혈약침이 자궁내막증 白鼠에 미치는 영향", 대한약침학회지, 9(1), pp.83~94 (2006), 대한약침학회
- 조현주, 김윤상, 임은미, "黃?가 자궁내막증 유발 흰쥐에 미치는 영향", 대한한방부인과학회지, 20(2), pp.43~59 (2007), 대한한방부인과학회
- 이미정, "八珍湯이 子宮內膜症을 유발한 흰쥐에 미치는 영향", 대한한방부인과학회지, 19(3), pp.83~94 (2006), 대한한방부인과학회
- 김성혜, "산삼약침이 자궁내막증 유발 흰쥐에 미치는 영향", 대한침구의학회지, 23(5), pp.55~67 (2006), 대한침구의학회
- 龔廷賢, "國譯 萬病回春 하권", pp.225~229 (1977), 계축문화사
- 許浚, "對譯 東醫寶鑑", pp.1628~ (1999), 법인문화사
- 정선례, "芎歸調血飮 煎湯液이 진통·진정·해열 및 지혈작용에 미치는 영향", 석사, pp.1987
- Vernon MW, "Studies on the surgical induction of endometriosis in the rat", Fertil Steril, 44(5), pp.684~694 (1985)
- Gazvani R, "The peritoneal environment, cytokines and angiogenesis in the pathophysiology of endometriosis", Reproduction, 123, pp.217~226 (2002)
- McLaren J, "Vascular endothelial growth factor and endometriotic angiogenesis", Human Reproduction Update, 6(1), pp.45~55 (2000)
- 郭靄春, "黃帝內經 靈樞校注語譯", pp.102~136 (1993), 도서출판 의성당
- 張介賓, "景岳全書", pp.886~887 (1991), 人民衛生出版社
- 박준식, "蒲公英 추출물이 자궁내막증 유발 白鼠에 미치는 영향", 대한한방부인과학회지, 19(3), pp.69~82 (2006), 대한한방부인과학회
- Donnez J, "Typical and subtle atypical presentations of endometriosis", Endoscopic surgery, 16, pp.431~437 (2004)
- 채우석, "면역질환의 한방개념과 치료에 관한 문헌적 고찰", 대한한의학회지, 11(2), pp.54~91 (1990)
- Barcz E, "Role of cytokines in pathogenesis of endometriosis", Med Sci Monit, 6(5), pp.1042~1046 (2000)
- Boucher A, "Ovarian hormones modulate monocyte chemotactic protein-1 expression in endometrial cells of women with endometriosis", Molecular Human Reproduction, 6(7), pp.618~626 (2000)
- Cheong YC, "IL-1, IL-6 and TNF-α concentrations in the peritoneal fluid of women with pelvic adhesions", Human Reproduction, 17(1), pp.69~75 (2002)
- Akoun A, "Secretion of interleukin-6 by human endometriotic cells and regulation by proinflammatory cytokines and sex steroids", Human Reproduction, 11(10), pp.2269~2275 (1996)
- Yoshida S, "A combination of interleukin-6 and its soluble receptor impairs sperm mobility : implications in infertility associated with endometriosis", Human Reproduction, 19(8), pp.1821~1825 (2004)
- 胡國珍, "活血化瘀法治療子宮內膜異位症 48例", 上海中醫藥雜誌, 2, pp.38~40 (1995)
- 李文芹, "活血化瘀法治療子宮內膜異位症 64例", 吉林中醫藥, 1, pp.33~ (1998)
- 王大增, "子宮內膜異位症的中醫藥診治", 中西醫結合雜誌, 2, pp.112~114 (1994)
- 錢靜, "桂枝茯苓丸加味治療子宮內膜異位症的臨床硏究", 楊洲大學醫學院, pp.170~ (1999)
- 오규석, "血府逐瘀湯이 자궁내막증 白鼠에 미치는 영향", 대한한의학회지, 18(2), pp.273~282 (1995)
- 김동일, "자궁내막종 환자 2례에 대한 임상적 고찰", 대한한의학회지, 18(2), pp.58~71 (1997)
- 권은정, "內膜Ⅰ號丸의 자궁내막증 白鼠의 치료효과에 대한 연구", 東國論集, 15, pp.325~342 (1996)
- 이동규, "內膜Ⅱ號丸의 phospholipase A2 활성 억제능에 관한 연구", 대한한방부인과학회지, 9(1), pp.97~107 (1996)
- 김동일, "자궁내막증 및 만성 지속성 간염과 병발한 원발성 불임증 1례에 대한 치험 보고", 대한한의학회지, 19(1), pp.100~108 (1998)
- 허 윤, 이은, 임은미, "丹參이 子宮內膜症 白鼠에 미치는 영향", 대한한방부인과학회지, 19(3), pp.95~108 (2006), 대한한방부인과학회
- 송미선, "半夏가 자궁내막증 유발 흰쥐에 미치는 영향", 대한한방부인과학회지, 20(2), pp.107~123 (2007), 대한한방부인과학회
- 이용현, "蜂藥鍼이 자궁내막증 白鼠에 미치는 영향", 대한약침학회지, 9(1), pp.21~32 (2006)
- 엄은석, "속발성 월경통을 한약 내복및 약물보류관장법으로 치료한 치험 2례", 대한한방부인과학회지, 12(1), pp.1~12 (1999)
- 조성희, 진천식, "慢性骨盤炎을 韓方治療한 治驗一例 ( 韓藥內服藥과 保留灌腸治療 중심으로 )", 대한한방부인과학회지, 15(3), pp.181~189 (2002), 대한한방부인과학회
- 조성희, 진천식, "骨盤炎 치료를 위해 사용된 保留灌腸療法에 關한 文獻的 考察(중국 잡지를 중심으로)", 대한한방부인과학회지, 15(2), pp.79~93 (2002), 대한한방부인과학회
- 전국한의과대학 본초학교실, "本草學", pp.136~580 (1998), 永林社
- 노영범, "腹診과 正統方劑學", pp.437~440 (2006), 대성의학사
- 김영아, "자궁내막증 자궁내막의 증가된 증식 활성도", 대한산부인과학회잡지, 47(7), pp.1334~1341 (2004), 대한산부인과학회
- 강정배, "자궁내막증환자 복강액의 세포 매개성 면역반응의 변화양상에 관한 연구", 대한산부회지, 42(9), pp.1965~1971 (1999)
- 김동호, "자궁내막증에서 MCP-1의 면역조직화학적 특성", 대한산부회지, 43(12), pp.2140~2145 (2000)
- 김호성, "자궁내막증의 존재유무에 따른 혈장과 복강액내의 TNF-α 농도 : TNF-α가 자궁내막증의 병인인가?", Obstetrics & Gynecology Science, 44(9), pp.1617~1620 (2001), 대한산부인과학회
- Bedaiwy MA, "Prediction of endometriosis with serum and peritoneal markers: a prospective controlled trial", Human Reproduction, 17(2), pp.426~431 (2002)
- Lebovic DI, "Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1β", Molecular Human Reproduction, 6(3), pp.269~275 (2000)
- Akoum A, "Estradiol and Interleukin-1β exert a synergistic stimulatory effect on the expression of the chemokine regulated upon activation, normal T cell expressed, and secreted in endometriotic cells", The Journal of Clinical Endocrinology & Metabolism, 87(12), pp.5785~5792 (2002)
- Cao WG, "Stimulation of macrophage migration inhibitory factor expression in endometrial stromal cells by Interleukin-1, beta involving the nuclear transcription factor NFKB", Biology of Reproduction, 73, pp.565~570 (2005)
- Meresman GF, "Effect of GnRH analogues on apoptosis and release of interleukin-1β and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis", Human Reproduction, 18(9), pp.1767~1771 (2003)
- 최두석, "자궁내막증 여성에서의 GnRH Agonist 투여후 말초혈액내 Cytokine 농도의 변화양상에 관한 연구", 대한산부회지, 42(5), pp.1030~34 (1999)
- Rier SE, "Dysregulation of interleukin-6 responses in ectopic endometrial stromal cells : correlation with decreased soluble receptor levels in peritoneal fluid of women with endometriosis", Journal of Clinical Endocrinology & Metabolism, 80(4), pp.1431~1437 (1995)
- 전상식, "난소관련병변에서 복강체액내의 cytokine농도", 대한산부인과학회잡지, 44(2), pp.258~262 (2001), 대한산부인과학회
- 이경석, "자궁내막증 환자에서 복강내의 IL-6와 IL-10의 변화양상에 관한 연구", 대한산부회지, 42(11), pp.2558~2563 (1999)
- Wu MH, "Distinct regulation of cyclooxygenase-2 by interleukin-1β in normal and endometriotic stromal cells", The Journal of Clinical Endocrinology & Metabolism, 90(1), pp.286~295 (2005)
- Sales KJ, "Cyclooxygenase enzymes and prostaglandins in pathology of the endometrium", Reproduction, 126, pp.559~567 (2003)
- Ota H, "Distribution of cyclooxygenase-2 in eutopic and ectopic endometrium in endometriosis and adenomyosis", Human Reproduction, 16(3), pp.561~566 (2001)
- Wu MH, "Distinct mechanisms regulate cyclooxygenase-1 and -2 in peritoneal macrophages of women with and without endometriosis", Molecular Human Reproduction, 8(12), pp.1103~1110 (2002)
- Fagotti A, "Analysis of cyclooxygenase-2 expression in different sites of endometriosis and correlation with clinico-pathological parameters", Human Reproduction, 19(2), pp.393~397 (2004)
- 이준서, "착상관련 질환 환자의 자궁내막에 있어서 프로게스테론 수용체, integrin β3, COX-2의 발현 양상", 대한산부회지, 43(6), pp.961~967 (2000)
- Fan QU, "Effects of Yiweining Recipe on expressions of metalloproteinase -2 and cyclooxygenase-2 mRNAs in ectopic endometrium of rats with endometriosis", Journal of Chinese Integrative Medicine, 4(6), pp.634~638 (2006)
- Donnez J, "Vascular endothelial growth factor(VEGF) in endometriosis", Human Reproduction, 13(6), pp.1686~1690 (1998)
- 정창원, "자궁내막증 환자에서 자궁내막의 VEGF 발현 양상", 석사, pp.2006
- Lin YJ, "Neutrophils and Macrophages promote angiogenesis in the early stage of endometriosis in a mouse model", Endocrinology, 147(3), pp.1278~1286 (2006)
- McLaren J, "Vascular endothelial growth factor is produced by peritoneal fluid macrophages in endometriosis and is regulated by ovarian steroids", The American Society for Clinical Investigation, 98(2), pp.482~489 (1996)
- Na YJ, "Effects of peritoneal fluid from endometriosis patients on the release of vascular endothelial growth factor by neutrophils and monocytes", Human Reproduction, 21(7), pp.1846~1855 (2006)
- Sharkey AM, "Vascular endothelial growth factor expression in human endometrium is regulated by hypoxia", The Journal of Clinical Endocrinology & Metabolism, 85(1), pp.402~409 (2000)
- 박창수, "자궁내막증 병인에 있어서 혈관내피세포 성장인자의 역할", 대한산부회지, 41(5), pp.1410~1420 (1998)
- Nguyen M, "Age-induced reprogramming of mast cell degranulation", The Journal of Immunology, pp.5701~5707 (2005)
- Xu X, "Role of mast cells and myofibroblasts in human peritoneal adhesion formation", Annals of Surgery, 236(5), pp.593~601 (2002)
