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半枝蓮과 항암제 병용 투여에 의한 암세포 성장 저해에 관한 연구

Increasing Effects of Apoptosis When Co-treated Scutellaria barbata D. Don. with Anti-cancer Drugs

이정원|권수경|김동일|성정석|남주영|전현익
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2009.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제22권 제1호|pp. 125-139

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참고문헌

키워드

ApoptosisHeLa cellCisplatinScutellaria Batatae D. Don
주제분류한의학

초록

Purpose: This experiment was designed to find out increasing effects of S. barbata. co-treatment with anti-cancer drugs at cancer cell's growth inhibition effect. Methods: Divergent observational study of the S. barbata. co-treatment with Cisplatin treatment on HeLa cell. Cell viability using MTT assay, Cell Culture and Cytotoxicity Studies, Cell Cycle Analysis, Annexin V-FITC/PI assay, Cell morphological assessment, PARP cleavage using Western blotting analysis when HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts. Results: When HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts, we found out viability of HeLa cell, changing in the distribution of cell cycle, Annexin V-FITC staining, DAPI staining, PARP clavage protein assay by Western-blot. So Scutellaria Barbata extracts have increased apoptosis Conclusion: When co-treated Scutellaria Barbata extracts with anti-cancer drugs, the anti-cancer effects were increased. We still not sure which constituent apoptosis at cancer cells and activates anti-cancer effects suppressing, but we believe that it'll be revealed here after with following experiments.

Abstract

Purpose: This experiment was designed to find out increasing effects of S. barbata. co-treatment with anti-cancer drugs at cancer cell's growth inhibition effect. Methods: Divergent observational study of the S. barbata. co-treatment with Cisplatin treatment on HeLa cell. Cell viability using MTT assay, Cell Culture and Cytotoxicity Studies, Cell Cycle Analysis, Annexin V-FITC/PI assay, Cell morphological assessment, PARP cleavage using Western blotting analysis when HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts. Results: When HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts, we found out viability of HeLa cell, changing in the distribution of cell cycle, Annexin V-FITC staining, DAPI staining, PARP clavage protein assay by Western-blot. So Scutellaria Barbata extracts have increased apoptosis Conclusion: When co-treated Scutellaria Barbata extracts with anti-cancer drugs, the anti-cancer effects were increased. We still not sure which constituent apoptosis at cancer cells and activates anti-cancer effects suppressing, but we believe that it'll be revealed here after with following experiments.

참고문헌 (39)

  1. 대한산부인과학회, "부인과학 제4판", pp.693~736 (2007), 고려의학
  2. 한의부인과학 편찬위원회, "한의부인과학 上", pp.303~307 (2002), 정담
  3. 송병기, "한방부인과학", pp.249~253 (1998), 행림출판사
  4. Tanakaa-Kagawa T, "Reduced sensitivity of HeLa cells to cis- platinum by imultaneous overexpression of copper, zinc-superoxide dismutase and catalase", Biochemical pharmacology, 57(5), pp.545~548 (1999)
  5. Mensner PW, , Advances In Pharmacology, 41, pp.461~499 (1997)
  6. Williams G, "Programmed cell death: apoptosis and oncogenesis", Cell, 65, pp.1097~1098 (1991)
  7. 안태규, "자궁경부암 세포주에서 Cisplatin에 대한녹차 추출물의 효과", Obstetrics & Gynecology Science, 49(3), pp.592~598 (2006), 대한산부인과학회
  8. JS Eun, "Cytotoxicity of reaction-precipitate from coptidis Rhzoma and Glycyrhizae Radix aqueous mixture", Korean Journal of Pharmacognocy, 25(3), pp.356~ (1994)
  9. JS Eun, "The combined effect of n-BuOH fraction of Ulmi cortex and anticancer drugs on cancer cell lines", Korean Journal of Pharmacognocy, 25(3), pp.144~152 (1994)
  10. RK Oldham, "Biological Response Modifiers", Journal of the National Cancer Institute, 70, pp.789~796 (1993)
  11. 조수인, "Beneficial Effect of Nodus Nelumbinis Rhizomatis Extract on Cisplatin-Induced Kidney Toxicity in Rats", 대한본초학회지, 18(4), pp.127~134 (2003), 대한본초학회
  12. 김창민, "중약대사전", pp.1501~1504 (1997), 정담
  13. 서진우, "半枝蓮이 난소암세포(SUN -251)의 성장억제에 미치는 영향", 대한한방부인과학회지, 12(1), pp.151~160 (1999)
  14. 권은정, "半枝蓮이 유방암에 미치는 영향에 관한 연구", 대한한방부인과학회지, 12(2), pp.148~182 (1999)
  15. Yin X, "Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549", Life Sci, 75(18), pp.2233~2244 (2004)
  16. TK Lee, "Inhibitory effects of S. barbata on human uterine leiomyomal smooth muscle cell proliferation through cell cycle analysis", Int Immunopharmacol, 4(3), pp.447~454 (2004)
  17. Sato Y, "Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus", J Ethnopharmacol, 72(3), pp.483~488 (2000)
  18. 김동일, "Inhibitory effect of the Scutellaria barbata Don water-extracts on the growth and DNA incorporation of human cancer cells", 대한한의학회지, 27(4), pp.162~173 (2006), 대한한의학회
  19. 王西京, "半枝蓮提取物對 H22 肝癌荷瘤小鼠化療的增效減毒作用", 中西醫結合學報, 6(7), pp.720~724 (2008)
  20. 王西京, "半枝蓮含葯血淸對肝癌 H22 細胞淍亡及錢粒体膜電位的影向", 中西醫結合學報, 6(8), pp.821~826 (2008)
  21. 이윤정, "半枝蓮이 자궁암세포(HeLa22918)의 성장억제에 미치는 영향", 대한한방부인과학회지, 12(1), pp.185~196 (1999)
  22. EK KIM, "Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line", Int J Mol Med, 20(1), pp.123~128 (2007)
  23. 하지연, "半枝蓮이 HeLa Cell의 增殖抑制와 死滅에 미치는 影響", 대한한방부인과학회지, 19(4), pp.47~60 (2006)
  24. YY Cha, "Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway", Clinica Chimica Acta, 348, pp.41~48 (2004)
  25. 조해중, "자궁경부암 세포주에서 활성산소종의 영향에 의한 Apoptosis를 통하여 세포성장을 억제하는 Cisplatin과 Berberine의 상승효과", 동의생리병리학회지, 21(4), pp.992~997 (2007), 대한동의병리학회
  26. 양정민, "山藥의 항산화 작용에 대한 단백질체 분석 연구", 대한한방부인과학회지, 21(2), pp.108~124 (2008), 대한한방부인과학회
  27. Ravi R, "Mechanism of cisplain ototoxicity. Antioxidant system", Pharmacology & Toxicology, 76, pp.386~394 (1995)
  28. Schmidt F, "Lovastatin and phenylacetate induce apoptosis, but not differentiation, in human malignant glioma cells", Acta Neuropathologica, 101, pp.217~224 (2001)
  29. Crown JP, "The platinum agents: a role in breast cancer treatment", Semin Oncol, 28, pp.28~37 (2001)
  30. Jordan P, "Carmo-Fonseca M. Molecular mechanisms involved in cisplatin cytotoxicity", Cell Mol Life Sci, 57, pp.1229~1235 (2000)
  31. 김현경, "시스플라틴과 녹차 추출물의 병용에 의한 세포성장 저해 효과", Journal of Gynecologic Oncology, 17(4), pp.347~353 (2006), 대한부인종양콜포스코피학회
  32. Tomei LD, "Inhibition of radiation induced apoptosis in vitro by tumor promoters", Biochem. Biophys. Res. Commun, 155, pp.324~ (1988)
  33. 고경희, "Cisplatin에 의한 HeLa세포의 세포고사에 미치는 활성산소종의 영향", Korean Journal of Obstetrics and Gynecology, 46(12), pp.10~10 (2003), 대한산부인과학회
  34. Joaquin A.M, "Functional decline in aging and disease: a role for apoptosis", J Am Geriatr Soc, 49(9), pp.1234~1240 (2001)
  35. Howard A, "Synthesis of desoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage", Heredity, 6, pp.261~ (1953)
  36. Minshull J, "The role of cyclin synthesis, modification and destruction in the control of cell division", J Cell Sci, 12, pp.77~97 (1989)
  37. Liu MJ, "Methylprotodioscin induces G2", M arrest and apoptosis in K562 cells with the hyperpolarization of mitochondria, 2005, pp.224(2), 229~241
  38. Hou R, "Diosgenin induces apoptosis in HeLa cells via activation of caspase pathway", Acta Pharmacol Sin, 25(8), pp.1077~1082 (2004)
  39. Hara S, "Over expression of inhibitor of caspase-3 activated deoxyribonuclease in human renal cell carcinoma cells enhances their resistance to cytotoxic chemotherapy in vivo", J Urol, 166, pp.2491~2494 (2001)

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