울금이 MDA-MB-231 세포 및 DMBA로 유발된 흰쥐의 유방암에 미치는 영향
Effects of Curcuma longa L . on MDA-MB-231 Human Breast Cancer Cells and DMBA-induced Breast Cancer in Rats
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Objectives: Breast cancer is the most common cancer among women and hasrapidly increasing rate annually. At present, western cancer therapies by surgery,radiation, and anticancer drug have not been fully effective. So many interestsare given to herbal medicine on cancer treatment recently. This study was designed to investigate the effects of Curcuma longa L. (CL) onMDA-MB-231 human breast cancer cells and DMBA-induced breast cancer in rats. Methods: In this experiment, MDA-MB-231 cells were cultured in cell cultureplates. 0.0625, 0.125, 0.25, 0.5, 1.0 mg/ml of CL extract were tested for their antiproliferativeeffects on MDA-MB-231 cells by MMT assay. And we induced breastcancer in rats. The changes in tumor's weight, and the effects on proliferations ofsplenocyte and thymocyte were investigated. Results: CL showed anti-proliferative effects on MDA-MB-231 cells in proportionto concentration of the CL. DMBA-induced breast cancer in rats, tumor's weightof the rat was not statistically significant, but showed a tendency to be reducedin the groups treated with CL. Proliferation rate of the rat's splenocyte andthymocyte increased in proportion to CL. In breast cancer tissue, expression ofER-α was weakened proportionately to the concentration of the CL. Conclusions: These data suggest that CL can prevent the proliferation ofbreast cancer, then CL is useful to treat patient with breast cancer.
Abstract
Objectives: Breast cancer is the most common cancer among women and hasrapidly increasing rate annually. At present, western cancer therapies by surgery,radiation, and anticancer drug have not been fully effective. So many interestsare given to herbal medicine on cancer treatment recently. This study was designed to investigate the effects of Curcuma longa L. (CL) onMDA-MB-231 human breast cancer cells and DMBA-induced breast cancer in rats. Methods: In this experiment, MDA-MB-231 cells were cultured in cell cultureplates. 0.0625, 0.125, 0.25, 0.5, 1.0 mg/ml of CL extract were tested for their antiproliferativeeffects on MDA-MB-231 cells by MMT assay. And we induced breastcancer in rats. The changes in tumor's weight, and the effects on proliferations ofsplenocyte and thymocyte were investigated. Results: CL showed anti-proliferative effects on MDA-MB-231 cells in proportionto concentration of the CL. DMBA-induced breast cancer in rats, tumor's weightof the rat was not statistically significant, but showed a tendency to be reducedin the groups treated with CL. Proliferation rate of the rat's splenocyte andthymocyte increased in proportion to CL. In breast cancer tissue, expression ofER-α was weakened proportionately to the concentration of the CL. Conclusions: These data suggest that CL can prevent the proliferation ofbreast cancer, then CL is useful to treat patient with breast cancer.
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