靈芝 추출물이 Rat fetus 두개골로부터 분리한 조골세포에 미치는 영향
The Effects of Ganoderma lucidum Extract on Osteoblast in Rat Fetus Calvarial Cells
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Objectives: In this study, the author aimed to evaluate the effect of EtOH extractof Ganoderma lucidum (GLE) on osteoblast proliferation in rat fetus calvarial cells. Methods: The osteoblast separated from rat fetus calvariae was cultivated for6~21 days and evaluated the cell function. After the addition of GLE on the culturemedium, we determined the effect of GLE on the cell viability, cell proliferation,bone matrix protein synthesis, alkaline phosphatase (ALP) activity, collagen synthesisand calcified nodule formation of the cultivated osteoblast. Results: GLE did not change the survival rate of rat calvarial osteoblast. GLEincreased the proliferation of rat calvarial osteoblast. GLE increased ALP activityof rat calvarial osteoblast. GLE increased bone matrix protein synthesis of ratcalvarial osteoblast. GLE increased collagen synthesis of rat calvarial osteoblast. GLE slightly affected calcified nodule formation of rat calvarial osteoblast. Conclusions: This study suggests that Ganoderma lucidum might improve theosteoporosis resulted from augmentation of osteoblast proliferation.
Abstract
Objectives: In this study, the author aimed to evaluate the effect of EtOH extractof Ganoderma lucidum (GLE) on osteoblast proliferation in rat fetus calvarial cells. Methods: The osteoblast separated from rat fetus calvariae was cultivated for6~21 days and evaluated the cell function. After the addition of GLE on the culturemedium, we determined the effect of GLE on the cell viability, cell proliferation,bone matrix protein synthesis, alkaline phosphatase (ALP) activity, collagen synthesisand calcified nodule formation of the cultivated osteoblast. Results: GLE did not change the survival rate of rat calvarial osteoblast. GLEincreased the proliferation of rat calvarial osteoblast. GLE increased ALP activityof rat calvarial osteoblast. GLE increased bone matrix protein synthesis of ratcalvarial osteoblast. GLE increased collagen synthesis of rat calvarial osteoblast. GLE slightly affected calcified nodule formation of rat calvarial osteoblast. Conclusions: This study suggests that Ganoderma lucidum might improve theosteoporosis resulted from augmentation of osteoblast proliferation.
참고문헌 (40)
- 대한내과학회, "Harrison's Principles of Internal Medicine", pp.2845~2847 (2010), 도서출판 MIP
- Cooper C, "Hip fracture in the elderly: a worldwide projection", Osteoporos Int, 2(6), pp.285~289 (1992)
- 보건복지부 질병관리본부 건강영양조사과, "2010국민건강통계", 보건복지부 건강정책과, 517~ (2011)
- Gabriel SG, "Vertebral fracture risk factors in postmenopausal women over 50 in Valencia", Bone, 52(1), pp.393~399 (2013)
- Kang HY, "Incidence and mortality of hip fracture among the elderly population in South Korea: a population -based study using the national health insurance claims data", BMC Public Health, 4(10), pp.230~ (2010)
- 대한한방부인과학회, "한방여성의학 상", pp.173~ (2012), 의성당
- 김덕구, "肉桂 추출물이 Rat fetus 두개골로부터 분리한 조골세포에 미치는 영향", 대한한방부인과학회지, 25(3), pp.61~70 (2012), 대한한방부인과학회[DOI]
- 서울대학교의과대학 내과학 교실, "임상 내과학", pp.1793~1801 (2004), 고려의학
- 하승우, "골다공증(골다공증의 치료: 골흡수 억제제)", pp.51~55 (1995), 경북대학교병원
- Estell R, "Bisphosphonates for postmenopausal osteoporosis", Bone, 49(1), pp.82~88 (2011)
- 정윤석, "골다공증의 최신 치료", 대한류마티스학회지, 19(1), pp.4~10 (2012), 대한류마티스학회
- 程士德, "內經", pp.198~ (2006), 人民衛生出版社
- 정우열, "한방임상병리학", pp.507~ (1998), 영림사
- 정승필, "노인에서의 골다공증의 진단 및 치료", 가정의학회지, 19(11), pp.1117~1124 (1998)
- 전국한의과대학 공동교재편찬위원회, "본초학", pp.538~539 (2008), 영림사
- 馬繼興, "神農本草經輯注", pp.84~88 (1995), 人民衛生出版社
- 이준호, "영지(靈芝)가 알코올 섭취한 흰쥐의 간 기능 및 지질대사에 미치는 영향", 한국영양학회지, 32(5), pp.519~525 (1999)
- 유재권, "노화촉진마우스의 기억력 및 산화 스트레스에 미치는 영지(Ganoderma lucidum) 추출물의 영향", 생명과학회지, 9(5), pp.548~555 (1999)
- 이병훈, "영지 약침액이 인체 위암 세포 성장억제 및 세포사멸 유발에 미치는 영향", Korean Journal of Acupuncture, 29(2), pp.271~289 (2012), 경락경혈학회[DOI]
- 강소신의학원, "중약대사전", pp.1180~1182 (1978), 상해 과학기술 출판사
- Futoshi S., "Steps to internal medicine part 9", pp.151~152 (2008), 도서출판 정담
- 지제근, "의학용어 큰 사전", pp.1281~1282 (2004), 아카데미아
- The Writing Group for the PEPI, "Effects of hormone therapy on bone mineral density: results from the postmenopausal estrogen", progestin interventions (PEPI) trial, 1996, pp.276(17), 1389~1396
- Favus MJ, "Bisphosphonates for osteoporosis", N Engl. J Med, 363(21), pp.2027~2035 (2010)
- Shane E, "Atypical subtrochanteric and diaphyseal femoral fractures: report of a task force of the American society for bone and mineral research", J Bone Miner Res, 25(11), pp.2267~2294 (2010)
- Rossouw JE, "Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women's health initiative randomized controlled trial", JAMA, 288(3), pp.321~333 (2002)
- 정호연, "골형성 촉진제 : 부갑상선호르몬과 스트론티움", 임상내과, 7(11), pp.65~72 (2009)
- "골다공증 치료에서 골형성 촉진제의 역할"[URL]
- 장재석, "골다공증 골절의 약물 요법", 대한골절학회지, 23(3), pp.326~340 (2010), 대한골절학회
- 김선호, "善呼靈樞", pp.167~ (2003), 주민출판사
- 김희진, "폐경기골다공증에 관한 문헌적 고찰", 대한한방부인과학회지, 11(1), pp.131~148 (1998)
- 이응세, "골다공증의 동서의학적 임상문헌에 관한 고찰", 한방재활의학회지, 7(1), pp.437~456 (1997)
- 곽한복, "녹용 물 추출물의 파골세포 분화 억제효과", 동의생리병리학회지, 22(4), pp.891~895 (2008), 대한동의병리학회
- 김주호, "우슬의 파골세포 분화 억제와 골 흡수 억제효과", 대한본초학회지, 25(1), pp.65~74 (2010), 대한본초학회
- 정연태, "두충의 물 추출물이 파골세포의 분화에 미치는 영향", 동의생리병리학회지, 23(3), pp.613~618 (2009), 대한동의병리학회
- 박선민, "三氣飮加味方이 파골세포의 분화 및 조골세포의 활성에 미치는 영향", 대한한방부인과학회지, 25(2), pp.23~42 (2012), 대한한방부인과학회[DOI]
- 안덕균, "한국본초도감", pp.724~ (2003), 교학사
- Brendan FB, "Roles for NF-κ B and c-Fos in osteoclasts", Journal of Bone and Mineral Metabolism, 23(1), pp.11~15 (2005)
- René SA, "The direct role of vitamin D on bone homeostasis", Archives of Biochemistry and Biophysics, 473(2), pp.225~230 (2008)
- Ivan W, "Effects of osteogenic protein-1 (OP-1, BMP-7) on bone matrix protein expression by fetal rat calvarial cells are differentiation stage specific", Journal of Cellular Physiology, 169(1), pp.115~125 (1996)