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황기맥문동탕이 에스트로겐 결핍 Rat의 체중 변화와 비만 관련 유전자 발현에 미치는 영향

Effects of Hwanggimacmundong-tang on Body Weight and Gene Expression in Obese Rats with Estrogen Deficiency

유정은|유동열|이혜인
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2018.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제31권 제2호|pp. 49-67|DOI https://doi.org/10.15204/jkobgy.2018.31.2.049

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참고문헌

키워드

obesitygene expressionmenopauseHwanggimacmundong-tangestrogen-deficiency
주제분류한의학

초록

Objectives : This study was conducted to assess the effects of Hwanggimacmundong-tang extract(HM) on obese rats with estrogen deficiency. Method : The experiments were performed with ovariectomized rats as estrogen-deficient obesity model. Rats were grouped NC(Normal Control Group; sham operation group), OC(Obesity Control Group; estrogen-deficient group), HML(20 mg/kg/day), HMH(100 mg/kg/day). HM was administered orally for six weeks. Body weights and serum lipid level were measured, and real-time PCR was performed to estimate the effect of HM on gene expression in liver. Results : HM decreased the total cholesterol and triglyceride in serum. And HM increased leptin, CPT1 gene expression in liver tissue of obese rats with estrogen deficiency. However HM decreased PPARγ, PGC-1α, HMGCR, CYP8B1, LPL, ACAT1, ACAT2, ApoB, ACOX gene expression in liver tissue of obese rats with estrogen deficiency. Conclusions : It is concluded that HM reduced body weight, and regulate gene expression related to lipid metabolism. These results indicate Hwanggimacmundong-tang that might have potential for treatment of obesity and complications during the menopause caused by estrogen-deficiency.

Abstract

Objectives : This study was conducted to assess the effects of Hwanggimacmundong-tang extract(HM) on obese rats with estrogen deficiency. Method : The experiments were performed with ovariectomized rats as estrogen-deficient obesity model. Rats were grouped NC(Normal Control Group; sham operation group), OC(Obesity Control Group; estrogen-deficient group), HML(20 mg/kg/day), HMH(100 mg/kg/day). HM was administered orally for six weeks. Body weights and serum lipid level were measured, and real-time PCR was performed to estimate the effect of HM on gene expression in liver. Results : HM decreased the total cholesterol and triglyceride in serum. And HM increased leptin, CPT1 gene expression in liver tissue of obese rats with estrogen deficiency. However HM decreased PPARγ, PGC-1α, HMGCR, CYP8B1, LPL, ACAT1, ACAT2, ApoB, ACOX gene expression in liver tissue of obese rats with estrogen deficiency. Conclusions : It is concluded that HM reduced body weight, and regulate gene expression related to lipid metabolism. These results indicate Hwanggimacmundong-tang that might have potential for treatment of obesity and complications during the menopause caused by estrogen-deficiency.

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