반총산의 항산화, 항염증, 항소양증, 항균효능에 관한 실험 연구
The Experimental Study on Antioxidant, Anti-inflammatory, Antipruritic and Antibacterial Effects of the Banchongsan(BCS)
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Objectives: Banchong-san (BCS) is a herbal formula composed of 13 korean medicinal herbs and is traditionally used to treat inflammatory diseases and pain. The object of this study was to research the antioxidant, anti-inflammatory, antipruritic and antimicrobial effects of the BCS in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: In this experiment, effects of BCS on the following four were measured as follows: (1) Anti-oxidative effects were evaluated by 1,1–diphenyl-2-picryl-hydrazyl (DPPH) Radical scavenging activity, 2,2–azino–bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) Radical scavenging activity. (2) Anti-inflammatory effects were evaluated by the production amount of Reactive oxygen species (ROS), Nitric oxide (NO), Interleukin-1β (IL-1β), Interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), Prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)(the previous two are “mRNA"), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (p38), inhibitor of nuclear factor kappa B (IκBα), nuclear factor kappa B (NF-κB) (the previous five are "Protein") in LPS-Stimulated RAW 264.7 cells. (3)Antipruritic effects were evaluated by the production amount of histamine, Leukotriene B4 (LTB4), LeukotrieneC4 (LTC4) Levels in phorbol 12-myristate 13-acetate(PMA)/ ionomycin-stimulated MC/9 mast cell. (4) Anti-microbial effects were evaluated by the growth suppression of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus niger. Results: The following results were obtained through each measurement: (1) DPPH Radical Scavenging Activity, ABTS Radical Scavenging Activity evoked a significant concentration-dependent increase. (2) ROS, NO, IL-1β, IL-6, TNF-α, PGE2 production amount, iNOS, COX-2 mRNA expression were significantly reduced in the BCS extraction group compared with the control group and significantly decreased the amount of ERK, JNK, p38, NF-κB Protein expression. The amount of IκB-α Protein Expression have increased significantly. (3) The amounts of histamine, LTB4, LTC4 were significantly decreased. (4) The antibacterial efficacy, BCS inhibited the growth of Escherichia coli, Pseudomonas aeruginosa at concentrations of 5 μg/ml, but did not suppress the growth of staphylococcus aureus and aspergillus niger. Conclusions: The experimental results show that BCS has anti-oxidant, anti-inflammatory, antipruritic and antimicrobial properties.
Abstract
Objectives: Banchong-san (BCS) is a herbal formula composed of 13 korean medicinal herbs and is traditionally used to treat inflammatory diseases and pain. The object of this study was to research the antioxidant, anti-inflammatory, antipruritic and antimicrobial effects of the BCS in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: In this experiment, effects of BCS on the following four were measured as follows: (1) Anti-oxidative effects were evaluated by 1,1–diphenyl-2-picryl-hydrazyl (DPPH) Radical scavenging activity, 2,2–azino–bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) Radical scavenging activity. (2) Anti-inflammatory effects were evaluated by the production amount of Reactive oxygen species (ROS), Nitric oxide (NO), Interleukin-1β (IL-1β), Interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), Prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)(the previous two are “mRNA"), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (p38), inhibitor of nuclear factor kappa B (IκBα), nuclear factor kappa B (NF-κB) (the previous five are "Protein") in LPS-Stimulated RAW 264.7 cells. (3)Antipruritic effects were evaluated by the production amount of histamine, Leukotriene B4 (LTB4), LeukotrieneC4 (LTC4) Levels in phorbol 12-myristate 13-acetate(PMA)/ ionomycin-stimulated MC/9 mast cell. (4) Anti-microbial effects were evaluated by the growth suppression of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus niger. Results: The following results were obtained through each measurement: (1) DPPH Radical Scavenging Activity, ABTS Radical Scavenging Activity evoked a significant concentration-dependent increase. (2) ROS, NO, IL-1β, IL-6, TNF-α, PGE2 production amount, iNOS, COX-2 mRNA expression were significantly reduced in the BCS extraction group compared with the control group and significantly decreased the amount of ERK, JNK, p38, NF-κB Protein expression. The amount of IκB-α Protein Expression have increased significantly. (3) The amounts of histamine, LTB4, LTC4 were significantly decreased. (4) The antibacterial efficacy, BCS inhibited the growth of Escherichia coli, Pseudomonas aeruginosa at concentrations of 5 μg/ml, but did not suppress the growth of staphylococcus aureus and aspergillus niger. Conclusions: The experimental results show that BCS has anti-oxidant, anti-inflammatory, antipruritic and antimicrobial properties.
참고문헌 (36)
- Korean Society of Obsterics & Gynecology, "Gynecology", pp.154~156 (2015), Korea Medical Book Publishing Company
- 조문경, "골반염 치료의 최신 지견", Obstetrics & Gynecology Science, 53(11), pp.961~966 (2010), 대한산부인과학회
- The Society of Korean Medicine Obstetrics & Gynecology, "Korean Medicine Obstetrics and Gynecology(下)", pp.331~344 (2016), Uiseongdang
- Kim JH, "Literature review on pelvic inflammatory disease", J Korean Obstet Gynecol, 7(1), pp.31~46 (1994)
- Yoon KY, "Korean Medicine Clinical Herbal Formula Science", pp.81~ (1989), Myeongbo Pubilsh
- Kee SJ, "Study on Applicable Pathology and Its Application for Banchong-san", Journal of Physiology & Pathology in Korean Medicine, 12(2), pp.27~32 (1998)
- 문혜연, "만성 골반통 여성의 한의학적 치료에 관한 국내 임상 연구 고찰", 대한한방부인과학회지, 31(3), pp.50~60 (2018), 대한한방부인과학회[DOI]
- Jang YH, "Effect of Banchong-san and Gamibanchong-san on bile secretion and analgesic action", Herbal formula science, 1(1), pp.103~125 (1990)
- Kim KR, "Effects of Banchong-san and Banchong-sankameebangs on the Pancreatitis Evoked by Trypsin", The Journal of Kyung Hee University Medical Center, 3(2), pp.268~280 (1987)
- 김윤근, "蟠？散이 子宮筋腫細胞의 死滅과 Cell Apoptosis 에 미치는 影響", 대한한방부인과학회지, 16(2), pp.34~44 (2003), 대한한방부인과학회
- Heo J, "Dongui bogam (東醫寶鑑)", pp.821~ (2005), Dongui bogam Publish
- Liu JY, "Taepyeonghyeminhwajegukbang (太平惠民和劑局方)", pp.127~128 (1985), People’s Medical Publishing House company
- Whang DY, "PangYakHapPyon(方藥合編)", pp.311~ (2003), Youngrim Publish
- The Compilation Committee of the teaching materials for Korean college of oriental medicine, "Herbal Medicine", pp.387~ (2005), Youngrim Publish
- The Korean Society of Gynecologic Endocrinology, "Gynecologic Endocrinology", pp.781~784 (2012), Koonja Publish
- Ljubin-Sternak S, "Chlamydia trachomatis and Genital Mycoplasmas : Pathogens with an Impact on Human Reproductive Health", Journal of Pathogens, 2014, pp.183167~ (2014)
- 오용택, "본초 구성을 이용한 방제 효능 예측 연구 및 본초의 효능 강도 설정안", 대한한의학원전학회지, 21(2), pp.49~58 (2008), 대한한의학원전학회
- Aldred EM, "Pharmacology A Handbook for Complementary Healthcare Professionals", pp.41~52 (2009), Elsevier
- Ray PD, "Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling", Cell Signal, 24(5), pp.981~990 (2012)
- Anija Y, "Oxidative stress-induced activation of microsomal glutathione S-transferase in isolated rat liver", Biochem Pharmacol, 45(1), pp.37~42 (1993)
- Lim DG, "Oxidative Stress; Reactive Oxygen Species and Nitric Oxide", Korean Society of Critical Care Medicine, 19(2), pp.81~85 (2004)
- Feldmann M, "Role of cytokines in rheumatoid arthritis", Annual Review of Immunology, 14(1), pp.397~440 (1996)
- Lopez-Castejon GR, "Understanding the mechanism of IL-1β secretion", Cytokine & Growth Factor Reviews, 22(4), pp.189~195 (2011)
- Tanaka TS, "IL-6 in Inflammation, Immunity, and Disease", Cold Spring Harb Perspect Biol, 6(10), pp.a016295~ (2014)
- Barner PJ, "The cytokine network in asthma and chronic obstructive pulmonary disease", J Cli Invest, 18(11), pp.3546~3556 (2008)
- Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37(6), pp.355~361 (2003)
- Lee AK, "Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κBαphosphorylation, C", EBP and AP-1activation, 2003, pp.139(1), 11~20
- Chen F, "New insights into the role of nuclear factor-kappaB in cell growth regulation", Am J Pathol, 159(2), pp.387~397 (2001)
- Xiong HZ, "Effects of taraxasterol on iNOS and COX-2 expression in LPS-induced RAW 264.7 macrophages", J Ethnopharmacol, 155(1), pp.753~757 (2014)
- Korean Society for Biochemistry and Molecular Biology, "Encyclopedia of Biochemistry", pp.123~ (2019), Korean Society for Biochemistry and Molecular Biology
- Church MK, "The human mast cell", J Allergy Clin Immunol, 99(2), pp.155~160 (1997)
- Li PP, "LTB4 causes macrophage -mediated inflammation and directly induces insulin resistance in obesity", Nat Med, 21(3), pp.239~247 (2015)
- Crooks SW, "Leukotriene B4", The International Journal of Biochemistry & Cell Biology, 30(2), pp.173~178 (1998)
- Kang YH, "Encyclopedia of Life Science", pp.504~ (2008), Academy books
- 이근하, "양마에서 분리한 Kaempferol과 그 배당체의 항균 및 주름억제 효과", 한국약용작물학회지, 20(6), pp.454~460 (2012), 한국약용작물학회[DOI]
- The Korean society of pathologists, "Textbook of pathology", pp.176~ (2017), Komoonsa