본문 바로가기
KERD
논문KCI 등재

The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Purpose: N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder. Materials and Methods: I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation. Results: In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group. Conclusion: Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.

Abstract

Purpose: N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder. Materials and Methods: I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation. Results: In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group. Conclusion: Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.

참고문헌 (34)

  1. Erdem E, "Effect of bladder ischaemia", reperfusion on superoxide dismutase activity and contraction, 2005, pp.96, 169~174
  2. Aikawa K, "Effect of age on hydrogen peroxide mediated contraction damage in the male rat bladder", J Urol, 170, pp.2082~2085 (2003)
  3. Bratslavsky G, "Effects of in vivo ischemia on contractile responses of rabbit bladder to field stimulation, carbachol, ATP and KCl", Pharmacol, 59, pp.221~226 (1999)
  4. Ohnishi N, "Effect of repetitive stimulation on the contractile response of rabbit urinary bladder subjected to in vitro hypoxia or in vitro ischemia followed by reoxygenation", Pharmacol, 57, pp.139~147 (1998)
  5. Bratslavsky G, "Reperfusion injury of the rat bladder is worse than ischemia", J Urol, 170, pp.2086~2090 (2003)
  6. Levin RM, "Effect of calcium and calcium chelator on the response of the bladder to in vitro ischaemia", Br J Urol, 82, pp.882~887 (1998)
  7. Kaneko H, "Proof of the mysterious efficacy of ginseng: basic and clinical trials: clinical effects of medical ginseng, korean red ginseng: specifically, its anti-stress action for prevention of disease", J Pharmacol Sci, 95, pp.158~162 (2004)
  8. Sugaya A, "Proliferative effect of ginseng saponin on neurite extension of primary cultured neurons of the rat cerebral cortex", J Ethnopharmacol, 22, pp.173~181 (1988)
  9. Hasegawa H, "Prevention of growth and metastasis of murine melanoma through enhanced natural-killer cytotoxicity by fatty acid-conjugate of protopanaxatriol", Biol Pharm Bull, 25, pp.861~866 (2002)
  10. Maffei Facino R, "Panax ginseng administration in the rat prevents myocardial ischemia-reperfusion damage induced by hyperbaric oxygen: evidence for an antioxidant intervention", Planta Med, 65, pp.614~619 (1999)
  11. Lee HJ, "Antioxidant effects of Korean red ginseng components on the antioxidant enzymes activity and liver peroxidation in the liver of mouse treated with paraquat", J Ginseng Research, 23, pp.8~ (1999)
  12. Jung NP, "Studies on the physiological and biochemical effects of Korean ginseng", Korean J Ginseng Sci, 20, pp.41~ (1996)
  13. Lee BM, "Inhibition of oxidative DNA damage, 8-OHdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, beta-carotene and red ginseng)", Cancer Lett, 132, pp.219~227 (1998)
  14. Kim YK, "Free radical scavenging activity of red ginseng aqueous extracts", Toxicology, 172, pp.149~156 (2002)
  15. Saito M, "Bladder dysfunction after acute urinary retention in rats", J Urol, 165, pp.1745~1747 (2001)
  16. Carpenter FG, "Impairment and restoration of rat urinary bladder responsiveness following distension", Am J Physiol, 244, pp.106~113 (1983)
  17. Lee TM, "Acute effects of urinary bladder distention on the coronary circulation in patients with early atherosclerosis", J Am Coll Cardiol, 36, pp.453~460 (2000)
  18. Tammela T, "Effect of distension on adrenergic innervation of the rat urinary bladder", Urol Res, 18, pp.345~348 (1990)
  19. Cassarino DS, "An evaluation of the role of mitochondria inneurodegenerative diseases: mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration", Brain Res Brain Res Rev, 29, pp.1~25 (1999)
  20. Lin AT, "Detrusor mitochondrial lipid peroxidation and superoxide dismutase activity in partial bladder outlet obstruction of rabbits", Neurourol Urodyn, 24, pp.282~287 (2005)
  21. Lin AT, "Correlation of lipid peroxidation with energetic metabolism and contractile function in overdistention of the rabbit urinary bladder", Neurourol Urodyn, 15, pp.6~ (1996)
  22. Gosling JA, "Correlation between the structure and function of the rabbit urinary bladder following partial outlet obstruction", J Urol, 163, pp.1349~1356 (2000)
  23. Lu SH, "Morphological and morphometric analysis of human detrusor mitochondria with urodynamic correlation after partial bladder outlet obstruction", J Urol, 163, pp.225~229 (2000)
  24. de Jongh R, "Oxidative stress reduces the muscarinic receptor function in the urinary bladder", Neurourol Urodyn, 26, pp.302~308 (2007)
  25. Bratslavsky G, "Urethra is more sensitive to ischemia than bladder: evidence from an in vitro rat study", J Urol, 165, pp.2086~2090 (2001)
  26. George J, "The role of urate and xanthine oxidase in vascular oxidative stress: future directions", Ther Clin Risk Manag, 5, pp.799~803 (2009)
  27. Didion SP, "Effects of NADH and NADPH on superoxide levels and cerebral vascular tone", Am J Physiol Heart Circ Physiol, 282, pp.688~695 (2002)
  28. Parks DA, "Xanthine oxidase: biochemistry, distribution and physiology", Acta Physiol Scand Suppl, 548, pp.87~99 (1986)
  29. McCord JM, "Oxygen-derived free radicals in postischemic tissue injury", N Engl J Med, 312, pp.159~163 (1985)
  30. Yokoyama Y, "Circulating xanthine oxidase: potential mediator of ischemic injury", Am J Physiol, 258, pp.564~570 (1990)
  31. Pacher P, "Therapeutic ef fects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol", Pharmacol Rev, 58, pp.87~114 (2006)
  32. Parekh MH, "Protective effect of vitamin E on the response of the rabbit bladder to partial outlet obstruction", J Urol, 166, pp.341~346 (2001)
  33. Conners W, "L-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction", Am J Physiol Renal Physiol, 290, pp.7~ (2006)
  34. Kim CS, "Effect of Korea red ginseng on cerebral blood flow and superoxide production", Acta Pharmacol Sin, 23, pp.1152~1156 (2002)

관련 논문AI추천

Mirodenafil Prevents Bladder Dysfunction Induced by Chronic Bladder Ischemia in Rats최훈, 문두건, 박재영, 배재현, 심지성, 이정구대한배뇨장애요실금학회 · International Neurourology Journal2015 · 제19권 제1호Metformin and Sildenafil Attenuate Inflammation and Suppress Apoptosis After Ischemia/Reperfusion Injuries in Rat Urinary Bladder김건화, 나용길, 박종목, 송기학, 신주현, 양승우, 이지용, 이충렬, 임재성대한배뇨장애요실금학회 · International Neurourology Journal2021 · 제25권 제4호인삼 사포닌이 하부요로와 음경해면체 평활근의 이완작용에 미치는 효과오태희, 정희창영남대학교 의과대학 · Journal of Yeungnam Medical Science2006 · 제23권 제1호Expression of Nitric Oxide Synthase and Aquaporin-3 in Cyclophosphamide Treated rat Bladder나용길, 송기학, 신주현, 장영섭, 조군현, 현재호대한배뇨장애요실금학회 · International Neurourology Journal2010 · 제14권 제3호Intravesical Instillation of Hyaluronic Acid With Epidermal Growth Factor for Restoring Urothelial Denudation and Alleviating Oxidative Stress in Lipopolysaccharide-Induced Interstitial Cystitis of RatsChih-Chieh Lin, Hua-Lin Lee, Jenn-Ming Yang, Tzu-Hsiang Hsu대한배뇨장애요실금학회 · International Neurourology Journal2024 · 제28권 제2호Overexpression of Aquaporin-1 and Caveolin-1 in the Rat Urinary Bladder Urothelium Following Bladder Outlet Obstruction권동득, 김선옥, 박광성, 송승희대한배뇨장애요실금학회 · International Neurourology Journal2013 · 제17권 제4호

이 간행물의 다른 논문 (14건)