Effects of various Herbal Acupuncture on Adjuvant Arthritis in Rats
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This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BVI, Bee Venom by Microwave stimulation in Korea; K-BVII, 0.5mg/㎖, Fu Yu Pharmaceutical Factory, China; C-BV, 1mg/㎖, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using Electrophoresis. The results were summarized as follows:1. In 1:4000 Bee Venom solution rate, the band was not displayed distinctly using Electrophoresis. But in 1:1000, the band showed clearly. 2. The results of Electrophoresis at solution rate 1:1000, K-BVIand K-BVII showed similar band. Phospholipase A2 of A-BV showed obscure band. 3. The molecular weight of Phospholipase A2 was known as 19,000 but its band was seen at 17,000 in Electrophoresis. 4. Protein concentration of Bee Venom by Lowry method was different at solution rate 1:4000 ; C-BV was 250㎍/㎖, K-BVI was 190㎍/㎖, K-BVII was 160㎍/㎖ and C-BV was 45㎍/㎖. 5. Electrophoresis method was unuseful for analysis of Bee Venom when solution rate is above 1:4000 but Protein concentration of Bee Venom by Lowry method was possible. These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.
Abstract
This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BVI, Bee Venom by Microwave stimulation in Korea; K-BVII, 0.5mg/㎖, Fu Yu Pharmaceutical Factory, China; C-BV, 1mg/㎖, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using Electrophoresis. The results were summarized as follows:1. In 1:4000 Bee Venom solution rate, the band was not displayed distinctly using Electrophoresis. But in 1:1000, the band showed clearly. 2. The results of Electrophoresis at solution rate 1:1000, K-BVIand K-BVII showed similar band. Phospholipase A2 of A-BV showed obscure band. 3. The molecular weight of Phospholipase A2 was known as 19,000 but its band was seen at 17,000 in Electrophoresis. 4. Protein concentration of Bee Venom by Lowry method was different at solution rate 1:4000 ; C-BV was 250㎍/㎖, K-BVI was 190㎍/㎖, K-BVII was 160㎍/㎖ and C-BV was 45㎍/㎖. 5. Electrophoresis method was unuseful for analysis of Bee Venom when solution rate is above 1:4000 but Protein concentration of Bee Venom by Lowry method was possible. These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.