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陰谷에 시술한 동과인 약침이 LPS로 유도된 흰쥐의 신장염에 미치는 영향

Anti-inflammatory Effect of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats

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초록

Objective: This study aimed to evaluate the effects of Benincasae Semen Herbal-acupuncture (BS-HA) at KI10 (Umgok) on nephritis induced by LPS in rat. Methods: Rats with nephritis induced by LPS, were treated with Benincasae Semen Herbal-acupuncture(BS-HA) injection at KI10. Two control groups, N.P. group and saline group, were treated with 26 gauge needle at KI10, 3 times a week. In Saline group normal saline was injected at KI10. To evaluate the effects of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats, WBC, Neutrophils in blood, BUN, Creatinine TNF-α, CINC-1 in serum, urinal volume and creatinine and Total protein in urine, reanl TNF-α, renal MPO were measured and reanl tissue was also analyzed. Results: BS-HA injected at KI10 significantly inhibited WBC and neutrophil in blood, creatinine in serum, and MPO in kidney of LPS-stimulated rats. BS-HA injected at KI10 reduced concentration of neutrophil in renal tissue of LPS-stimulated rats. Conclusion: BS-HA at KI10 has a therapeutic effect for nephritis in LPS-stimulated rat. Therefore, it is suggested that BS-HA at KI10 may be an useful therapeutics in clinical field after further researches.

Abstract

Objective: This study aimed to evaluate the effects of Benincasae Semen Herbal-acupuncture (BS-HA) at KI10 (Umgok) on nephritis induced by LPS in rat. Methods: Rats with nephritis induced by LPS, were treated with Benincasae Semen Herbal-acupuncture(BS-HA) injection at KI10. Two control groups, N.P. group and saline group, were treated with 26 gauge needle at KI10, 3 times a week. In Saline group normal saline was injected at KI10. To evaluate the effects of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats, WBC, Neutrophils in blood, BUN, Creatinine TNF-α, CINC-1 in serum, urinal volume and creatinine and Total protein in urine, reanl TNF-α, renal MPO were measured and reanl tissue was also analyzed. Results: BS-HA injected at KI10 significantly inhibited WBC and neutrophil in blood, creatinine in serum, and MPO in kidney of LPS-stimulated rats. BS-HA injected at KI10 reduced concentration of neutrophil in renal tissue of LPS-stimulated rats. Conclusion: BS-HA at KI10 has a therapeutic effect for nephritis in LPS-stimulated rat. Therefore, it is suggested that BS-HA at KI10 may be an useful therapeutics in clinical field after further researches.

참고문헌 (29)

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